Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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The Effect of Supplementary Feeding of Tannic acid on Growth and Hematological Changes in Rats Fed High Fat Diet

탄닌산(tannic acid) 첨가가 고지방 식이 흰쥐의 성장과 혈액학치 및 혈액화학치의 변화에 미치는 영향

  • Hwang Eui-kyung (Department of Animal Science, College of Life Science and Natural Resources, Sangji University)
  • 황의경 (상지대학교 생명자원과학대학 동물자원학과)
  • Published : 2005.06.01
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Abstract

This study was performed to investigate the growth rate, hematological and serological changes of the rats when they were fed with the high fat diets supplemented with or without the tannic acid for five weeks. Thirty-two Sprague-Dawley male rats(235.7\pm10.7g\;of\;body\;weight)$ were randomly divided into four groups, control group and three treatment groups(T1, T2 and T3). Rats in control group were fed with the high fat diet containing $15\%\;lard,\;1\%$ cholesterol and $0.5\%$ sodium cholate(wt/wt) which was modified from the formula of American Institute of Nutrition (AIN)-76 diet and rats in treatment groups were fed with above diet supplemented with $0.25\%(T1),\;0.5\%(T2)$ or $0.75\%(T3)$ of tannic acid(wt/wt), respectively. The supplementation of tannic acid(TA) did not affect the final body weight, gain of body weight and feed intake of rats in both control and treatment groups. The numbers of red blood cells, hemoglobin concentrations and hematocrit values in blood of rats showed no significant differences between control group and treatment groups. The glucose concentration and albumin/globulin(A/G) ratio of rats in treatment groups were slightly lower than that of control group without significance. The values of total protein, albumin and globulin showed no significant differences between control group and treatment groups. The values of total cholesterol, low density lipoprotein­cholesterol and atherogenic index in sera of rats in treatment groups were much lower than that of control group without significance. The values of triglycerides in sera of rats in T3 group were significantly lower than that of control group (p<0.05). The values of AST and ALT in sera of rats in T3 group were significantly lower than that of control group (p<0.05). Thus supplementation of tannic acid to high fat diet could be effective to reduce the serum lipid levels such as total cholesterol, high density lipoprotein-cholesterol and triglycerides which were regarded as to cause the cardiovascular diseases.

Keywords

References

  1. Chang MCJ, Bailey JW, Collins JL. Dietary tannins from cowpeas and tea transiently alter apparent calcium absorption but not absorption and utilization of proteins in rats. J Nutr 1994; 124: 283-288
  2. Chung KT, Wei CI, Johnson MG. Are tannins a double-edged sword in biology and health? Trends Food Sci Technol 1998; 9: 168-175 https://doi.org/10.1016/S0924-2244(98)00028-4
  3. Duncan DB. Multiple range and multiple F tests. Biometrics 1955; 11: 1-42 https://doi.org/10.2307/3001478
  4. Fickel J, Pitra Ch, Joest BA, Hofmann. A novel method to evaluate the relative tannin-binding capacities of salivary proteins. Comp Biochem Physiol Part C 1999; 122: 225-229
  5. Friewald, W. T., Levy, R. I. and Fredrickson, D. S. Estimation of concentration of low density lipoprptein cholesterol without use of the preparation of ultracentrifuge. Clin Chem. 1972; 28: 499-502
  6. Fukuyo M, Hara Y, Muramatsu K. Effect of tea leaf catechin, (-) . epigallocatechin gallate, on plasma cholesterol level in rats. J Jpn Soc Nutr Sci 1986; 39: 495-500 https://doi.org/10.4327/jsnfs.39.495
  7. Hagerman AE, Robbins CT, Weerasuriya Y, Wilson TC, McArthur C. J Range Manage 1992; 45: 57-62 https://doi.org/10.2307/4002526
  8. Hglund O, Luostarinen R, Wallin R, Wibell L, Saldeen T. The effects of fish oil on triglyceride cholesterol, fibrinogen and malondialdehyde in humans supplemented with vitamin E. J Nutr 1991; 121: 165-169
  9. Hikino H, Kiso Y, Hatano T, Yoshida T, Okuda T. Antihepatotoxic actions of tannins. J Ethnopharmacol 1985; 14: 19-29 https://doi.org/10.1016/0378-8741(85)90024-8
  10. Holmes DL. Clinical laboratory animal medicine. mes: lowa State University Press. 1984: 110-111
  11. Joslyn MA, Glick Z. Comparative effects of gallotanic acid and related phenolics on the growth of rats. J Nutr 1969; 98: 119-126
  12. Kaosar A, Kazuki S, satoshi I, Hiroshi H. Reducing effect of ingesting tannic acid on the bsorption of iron, but not of zinc, copper and manganese by rats. Biosci Biotechnol Biochem 2004; 68: 584-592 https://doi.org/10.1271/bbb.68.584
  13. Khan NS, Ahmad A, Hadi SM. Anti-oxidant, pro-oxidant properties of tannic acid and its binding to DNA. Chemico-Biological Interactions 2000; 125: 177-189 https://doi.org/10.1016/S0009-2797(00)00143-5
  14. Liu JC, Hsu FL, Tsai JC, Chan P, Liu JYH, Thomas GN, Tomlinson B, Lo MY, Lin JW. Antihypertensive effects of tannins isolated from traditional Chinese herbs as non-specific inhibitors of angiotensin converting enzyme. Life Sciences 2003; 73: 1543-1555 https://doi.org/10.1016/S0024-3205(03)00481-8
  15. Mitruka BM, Rawnsley HM. Clinical biochemical and hematological reference values in normal experimental animals and normal humans. 2nd ed. New York: Masson Publising USA, Inc. 1981:57-166
  16. Muramatsu K, Fukuyo M, Hara Y. Effect of green tea catechins on plasma cholesterol leel in cholesterol-fed rats. J Nutr Sci Vitaminol 1986; 32: 613-622 https://doi.org/10.3177/jnsv.32.613
  17. Niho N, Shibutani M, Tamura T, Toyoda K, Uneyaa C, Takahashi N, Hirose M. Subchronic toxicity study of gallic acid by oral administration in F344 rats. Food Chem Toxicol 2001; 39: 1063-1070 https://doi.org/10.1016/S0278-6915(01)00054-0
  18. Osweiler GD, Carson TL, Buck WB, Van Gelder GA. Oak poisoning. In: Clinical and diagnostic veterinary toxicology, 3rd ed. Dubuque: Kendall/Hunt Publising Company. 1985: 468-470
  19. Packard CJ, McKinney L, Carr K, Shepherd J. Cholesterol feeding increases low density lipoprotein synthesis. J Clin Invest 1983; 72: 45-51 https://doi.org/10.1172/JCI110983
  20. Schofield P, Mbugua DM, Pell AN. Analysis of condensed tannins: a review. Animal Feed Sci Technol 2001; 91: 21-40 https://doi.org/10.1016/S0377-8401(01)00228-0
  21. Spady DK, Woollett LA, Dietschy JM. Regulation of plasma LDL-cholesterol levels by dietary cholesterol and fatty acids. Annu Rev Nutr 1993; 13: 355-381 https://doi.org/10.1146/annurev.nu.13.070193.002035
  22. Tamir M, Alumot E. Carob tannins-Growth depression and levels of insoluble nitrogen in the digestive trct of rats. J Nutr 1970; 100: 573-580
  23. The American Institute of Nutrition : Report of American Institue of Nutrition Ad Hoc Committee on Standards for Nutritional Studies. J Nutr 1977; 107:1340-1348
  24. Wu?rsch P. Influence of tannin-rich Carob Pod fiber on the cholesterol metabolism in the rat. J Nutr 1979; 109: 685-692
  25. Yugarani T, Tan BKH, Teh M, Das NP. Effects of polyphenolic natural products on the lipid profiles of rats fed high fat diets. Lipids 1992; 27: 181-186 https://doi.org/10.1007/BF02536175
  26. Yugarani T, Tan BKH, Teh M, Das NP. The effects of tannic acid on serum lipid parameters and tissue lipid peroxidases in the spontaneously hypertensive and Wistar Kyoto rats. Planta Med 1993; 59: 28-31 https://doi.org/10.1055/s-2006-959598
  27. Zhu M, Phillipson JD, Greengrass PM, Bowery NE, Cai Y. Plant polyphenols: Biologically active compounds or non-selective binders to protein. Phytochemistry 1997; 44: 441-447 https://doi.org/10.1016/S0031-9422(96)00598-5
  28. 김형진, 송시환, 하창수, 한상섭. 시육밀도가 Sprague-Dwley 랫드의 성장 및 각종 생리치에 미치는 영향, 한국실험동물학회지 1993; 9: 71-82
  29. 서화중, 흰쥐에 마늘 투여로 혈액의 지질량과 그 외 혈액성분 변화 고찰. 한국식품영양과학회지 1999; 28: 1339-1348
  30. 서지형, 정용진, 김광수. 떫은 감에서 분리한 탄닝성분의 기능적 특성. 한국식품과학회지 2000; 32: 212-217
  31. 성인숙, 김명주, 조수열 : 도토리추출물이 흰쥐의 체내 지질대사에 미치는 영향, 한국식품영양과학회지 1997; 26: 327-333
  32. 송창우, 황화선, 한상섭. Ktc: SD 랫드의 주령에 따른 기초 연구 I. 체중변화, 혈액.혈액생화학적 변화 및 뇨분석. 한국실험동물학회지 1990; 6: 33-43
  33. 신미경, 한성희, 한경조. 녹차의 음용이 지방과 콜레스테롤을 투여한 흰쥐의 혈청지질 및 간의 조직에 미치는 영향. 한국영양식품과학회지 1997; 29: 1255-1263
  34. 육근정, 이혜진, 김미경. 밤과 도토리의 과육 및 내피가 흰쥐의 지방대사, 항산화능 및 항혈전능에 미치는 영향. 한국영양학회지 2002; 35: 171-182
  35. 이민경, 이세윤, 안형수, 안령미. 자외선B파로 유도된 hairless mouse의 과산화지질 및 항산화효소활성도와 탄닌의 효과. 한국환경위생학회지 1996; 22: 102-108
  36. 이삼열, 임상병리검사법. 서울: 연세대학교 출판부. 1979: 72-73
  37. 이삼열, 정윤섭, 권오헌, 송경순. 임상병리검사법. 7판. 서울: 연세대학교 출판부. 2000: 224-315
  38. 이승호, 박지수, 김소영, 정시련, 최상운. 유포르비아속 식물로부터 단리한 가수분해형 탄닌의 인체고형암 세포에 대한 세포독성효과. 약학회지 1997; 41: 524-529
  39. 한정순, 한용봉. 고지방식이와 식이섬유가 흰쥐의 체내 지질대사에 미치는 영향. 한국영양식량학회지 1994; 23: 541-547