한국임상수의학회지 (Journal of Veterinary Clinics)

  • 제22권2호
  • /
  • Pages.84-89
  • /
  • 2005
  • /
  • 1598-298X(pISSN)
  • /
  • 2384-0749(eISSN)
한국임상수의학회 (The Korean Society of Veterinary Clinics)
권호 표지

1,2-benzopyrone이 개 말초혈액 백혈구의 유주활성에 미치는 영향

Effect of 1,3-Benzopyrone on Chemotactic Activity of Peripheral Blood Leukocytes in the Dog

  • 김지현 (충북대학교 수의과대학 수의학과 및 동물의학연구소) ;
  • 강지훈 (충북대학교 수의과대학 수의학과 및 동물의학연구소) ;
  • 양만표 (충북대학교 수의과대학 수의학과 및 동물의학연구소)
  • Kim Jee-hyun (Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, and Research Institute of Veterinary Medicine, Chungbuk National University) ;
  • Kang Ji-houn (Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, and Research Institute of Veterinary Medicine, Chungbuk National University) ;
  • Yang Mhan-pyo (Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, and Research Institute of Veterinary Medicine, Chungbuk National University)
  • 발행 : 2005.06.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

개 말초혈액 백혈구의 유주성에 있어서 1,2-benzopyrone의 효과를 검토하였다. 백혈구의 유주성은 Boyden chamber 변법으로 측정하였다. 1.2-benzopyrone 그 자체는 PMN과 PBMC의 유주성에 직접적인 효과를 보이지 않았다. PMN과 PBMC는 1,2-benzopyrone으로 배양한 PMN의 배양상층액에 대해서도 유주활성을 보이지 않았다. 그러나 1,2-benzopyrone으로 배앙한 PBMC의 배양상층액은 PBMC에 대해서는 유주활성을 보이지 않았으나 PMN에 대해서는 현저한 유주활성을 나타내었다. 또한 유주인자인 IL-8에 의한 PMN의 유주활성 측정 결과도 1,2-benzopyrone으로 배양한 PBMC의 배양상충액의 그것과 유사하였다. 이러한 유주활성은 anti-IL-8 pAb를 처리했을 때 PMN의 유주활성이 억제되어, 본 유주활성은 PBMC에서 분비되는 IL-8일 것으로 강하게 시사되었다. 이상의 결과로부터, 1,2-benzopyrone은 개 말초혈액 PMN의 유주성에 대하여 면역자극 작용을 가지고 있으며, 이것은 1,2-benzopyrone의 자극에 의해 PBMC에서 분비되는 IL-8양 가용성 물질에 의해 나타나는 것으로 사료되었다

This study was undertaken to examine whether 1,2-benzopyrone affects on chemotactic activity of canine peripheral blood leukocytes. A modified Boyden chamber method was sed on chemotaxis evaluation. The direct treatments of 1,2-benzopyrone showed no ffects on the chemotaxis of peripheral blood mononuclear cells (PBMCs) and olymorphonuclear cells (PMNs). But chemotaxis of PMN was remarkably enhanced by ulture supernatant from PBMC but not PMN treated with 1,2-benzopyrone. Similarly, it as also increased by recombinant (r) interleukin (lL)­8. This chemotactic activity of MN was inhibited by addition of anti-rIL-8 polyclonal antibody. The chemotaxis of PBMC was not enhanced by culture supernatant from either PBMC or PMN treated with 1,2-benzopyrone. Therefore, these results suggested that the chemotactic activity of PMN ay be mainly mediated by IL-8-like factor(s) produced from PBMC treated with ,2-benzopyrone.

키워드

참고문헌

  1. Alam R, York J, Boyars M, Stafford S, Grant JA, Lee J, Forsythe P, Sim T, Ida N. Increased MCP-1, RANTES, and MIP-lalpha in bronchoalveolar lavage fluid of allergic asthmatic patients. Am J Respir Crit Care Med 1996; 153: 1398-1404 https://doi.org/10.1164/ajrccm.153.4.8616572
  2. Baggiolini M, Clark-Lewis I. Interleukin-8, a chemotactic and inflammatory cytokines. FEBS Lett 1992; 307: 97-101 https://doi.org/10.1016/0014-5793(92)80909-Z
  3. Bashir S, Cardigan R. Granulocyte concentrates: how can we assess their quality? Transfus Med 2003; 13: 245-257 https://doi.org/10.1046/j.1365-3148.2003.00451.x
  4. Bazzoni F, Cassatella MA, Rossi F, Ceska M, Dewald B, Baggiolini M. Phagocytosing neutrophils produce and relese high amounts of the neutrohil-activating peptide 1/interleukin-8. J Exp Med 1991; 173: 771-774 https://doi.org/10.1084/jem.173.3.771
  5. Bizzarri C, Pagliei S, Brandolini L, Mascagni P, Caselli G, Transidico P, Sozzani S, Bertini R. Selective inhibition of interleukin-8-induced neutrophil chemotaxis by ketoprofen isomers. Biochem pharmacol 2001; 61: 1429-1437 https://doi.org/10.1016/S0006-2952(01)00610-4
  6. Casley-Smith JR, Gaffney RM. Excess plasma proteins as a cause of chronic inflammation and lymphoedema: quatitative electron microscopy. J Pathol 1981; 133: 243-272 https://doi.org/10.1002/path.1711330307
  7. Casley-Smith JR, Wang CT, Zi-Hai C. Treatment of filarial lymphoedema and elephantiasis with 5,6-benzo-alpha-pyrone(coumarin). BMJ 1993; 307: 1037-1041 https://doi.org/10.1136/bmj.307.6911.1037
  8. Casley-Smith JR, Window J. Quantitative morphological correlations of alterations in capillary permeability, following histamine and moderate burning, in the mouse diaphragm; and the effects of benzopyrones. Microvasc Res 1976; 11: 279-305 https://doi.org/10.1016/0026-2862(76)90059-5
  9. Cohen AJ Critical review of the toxicology of coumarin with special reference to interspecies differences in metabolism and hepatotoxic response and their significance to man. Food Cosmet Toxicol 1979; 17: 277-289 https://doi.org/10.1016/0015-6264(79)90289-X
  10. Darbonne WC, Rice GC, Baker JB. Red blood cells are a sink for interleukin 8, a leukocyte Chemotxin. J Clin Invest 1991; 88: 1362-1369 https://doi.org/10.1172/JCI115442
  11. Detmers PA, Lo SK, Olsen-Egbert E, Walz A, Baggiolini M, Cohn ZA. Neutrophil-activating protein 1/interleukin 8 stimulates the binding activity of the leukocye adhesion receptor CD11b/CD18 on human neutrophils. J Exp Med 1990; 171: 1155-1162 https://doi.org/10.1084/jem.171.4.1155
  12. Egan D, O'Kennedy R, Moran E, Cox D, Prosser E. Thornes RD. The pharmacology, metabolism, analysis, and applications of coumarin and coumarin-related compounds. Drug Metab Rev 1990; 22: 503-529 https://doi.org/10.3109/03602539008991449
  13. Feldmann M, Brennan FM, Maini RN. Rheumatoid arthritis. Cell 1996; 85: 307-310 https://doi.org/10.1016/S0092-8674(00)81109-5
  14. Goldsby RA, Kindt TJ, Osborne BA. Leukocyte migration and inflammation, In: Kuby immunology, 4th ed. New York: W.H. Freeman and Company. 2000: 371-393
  15. Hirota Y, Yang MP, Araki S, Mohamed A, Matsumoto Y, Onodera T, Sugii S, Akiyama K. Immunostimulating activities of chicken egg white derivatives (EWD) and recombinant bovine interferon alpha 1 (rBoIFNal) in dogs and cattle. Bull Soc Fr-Jpn Sci Vet 1995; 6: 58-67
  16. Hirota Y, Yang MP, Araki S, Yoshihara K, Furusawa S, Yasuda M, Mohamed A, Matsumoto Y, Onodera T. Enhancing effects of chicken egg white derivtives on the phagocytic response in the dog. J Vet Med Sci 1995; 57: 825-829 https://doi.org/10.1292/jvms.57.825
  17. Hoult JR, Paya M. Pharmacological and biochemical actions of simple coumarins: natural products with therapeutic potential. Gen Pharmacol 1996; 27: 713-722 https://doi.org/10.1016/0306-3623(95)02112-4
  18. Ishikawa J, Suzuki S, Hotta K, Hirota Y, Mizuno S, Suzuki K. Cloning of a canine gene homologous to the human interleukin-8-encoding gene. Gene 1993; 131: 305-306 https://doi.org/10.1016/0378-1119(93)90313-R
  19. Kharazmi A, Nielsen H, Bendtzen K. Recombinant interleukin l Alpha and Beta prime human monocyte superoxide production but have no effect on chemotaxis and oxidative burst response of neutrophils. Immunobiology 1988; 177: 32-39 https://doi.org/10.1016/S0171-2985(88)80089-5
  20. Kim Ha, Kang JH, Yang MP. Immunostimulating effect of 1,2-benzopyrone on the oxidative burst activity to phagocytic response of canine peripheral blood phagocytes, J Vet Clin 2004; 21: 236-242
  21. Lee JK, Yang MP. Interleukin-8-like chemotactic factor from feline peripheral blood mononuclear cells cultured with egg white derivatives. Korean J Vet Res 2000; 40: 393-401
  22. Peveri P, Walz A, Dewald B, Baggiolini M. A novel neutrophil-activating factor produced by human mononuclear phagocytes. J Exp Med 1988; 167: 1547-1559 https://doi.org/10.1084/jem.167.5.1547
  23. Schmidt JA. Purification and partial biochemical chrcterization of normal human interleukin-1. J Exp Med 1984; 160: 772-787 https://doi.org/10.1084/jem.160.3.772
  24. Schratzberger P, Dunzendorfer S, Reinisch N, Kahler CM, Wiedermann CJ. Interleukin-8-induced human peripheral blood B-lymphocyte chemotaxis in vitro. Immunol Lett 1997; 58: 167-170 https://doi.org/10.1016/S0165-2478(97)00085-0
  25. Schratzberger P, Dunzendorfer S, Reinisch N, Kahler CM, Herold M, Wiedermann CJ. Release of chemoattractants for human monocytes from endothelial cells by interaction with neutrophils. Cardiovasc Res 1998; 38: 516-521 https://doi.org/10.1016/S0008-6363(98)00014-5
  26. Sin JH, Na KJ, Yang MP. Immunostimulating effect of 1,2-benzopyrone on phagocytic response of canine peripheral blood phagocytes. J Vet Clin 2004; 21: 23-28
  27. Strieter RM, Koch AE, Antony VB, Fick RB Jr, Standiford TJ, Kunkel SL. The immunopathology of chemotactic cytokines: the role of interleukin-8 and monocyte chemoattrctant protein-1. J Lab Clin Med 1994; 123: 183-197
  28. Suzuki.T, Tsuzuki A, Ohno N, Ohsima Y, Yadomae T. Enhancement of IL-8 production from human monocytic and granulocytic cell lines, THP-l and HL-60, stimulated with Malassezia furfur. FEMS Immunol Med Microbiol 2000; 28: 157-162 https://doi.org/10.1111/j.1574-695X.2000.tb01471.x
  29. Walz A, Peveri P, Aschauer H, Baggiolini M. Purification and amino acid sequencing of NAF, a novel neutrophil-activating factor produced by monocytes. Biochem Biophys Res Commun 1987; 149: 755-761 https://doi.org/10.1016/0006-291X(87)90432-3
  30. Watanabe K, Nakagaw H, Tsurufuji S. A new simple plastic chemotxis device of the Boyden chamber type utilizing an immunoassay plate. Jpn J Pharmacol 1985; 39: 102-104 https://doi.org/10.1254/jjp.39.102
  31. Yang MP, Lee JK. Immunostimulating effect of chicken egg white derivatives on chemotactic activity of feline peripherl blood polymorphonuclear cells. Korean J Vet Clin Med 2000; 17: 21-27
  32. Yang MP, Lee KJ, Yun SM, Kim JH, Ko IK, Jeung EB. Feline interleukin-8 expression in peripheral blood mono-nuclear cells induced by egg white derivatives. Vet Immunol Immunopathol 2002; 86: 43-53 https://doi.org/10.1016/S0165-2427(02)00010-7