Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Effect of 1,3-Benzopyrone on Chemotactic Activity of Peripheral Blood Leukocytes in the Dog

1,2-benzopyrone이 개 말초혈액 백혈구의 유주활성에 미치는 영향

  • Kim Jee-hyun (Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, and Research Institute of Veterinary Medicine, Chungbuk National University) ;
  • Kang Ji-houn (Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, and Research Institute of Veterinary Medicine, Chungbuk National University) ;
  • Yang Mhan-pyo (Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, and Research Institute of Veterinary Medicine, Chungbuk National University)
  • 김지현 (충북대학교 수의과대학 수의학과 및 동물의학연구소) ;
  • 강지훈 (충북대학교 수의과대학 수의학과 및 동물의학연구소) ;
  • 양만표 (충북대학교 수의과대학 수의학과 및 동물의학연구소)
  • Published : 2005.06.01
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Abstract

This study was undertaken to examine whether 1,2-benzopyrone affects on chemotactic activity of canine peripheral blood leukocytes. A modified Boyden chamber method was sed on chemotaxis evaluation. The direct treatments of 1,2-benzopyrone showed no ffects on the chemotaxis of peripheral blood mononuclear cells (PBMCs) and olymorphonuclear cells (PMNs). But chemotaxis of PMN was remarkably enhanced by ulture supernatant from PBMC but not PMN treated with 1,2-benzopyrone. Similarly, it as also increased by recombinant (r) interleukin (lL)­8. This chemotactic activity of MN was inhibited by addition of anti-rIL-8 polyclonal antibody. The chemotaxis of PBMC was not enhanced by culture supernatant from either PBMC or PMN treated with 1,2-benzopyrone. Therefore, these results suggested that the chemotactic activity of PMN ay be mainly mediated by IL-8-like factor(s) produced from PBMC treated with ,2-benzopyrone.

개 말초혈액 백혈구의 유주성에 있어서 1,2-benzopyrone의 효과를 검토하였다. 백혈구의 유주성은 Boyden chamber 변법으로 측정하였다. 1.2-benzopyrone 그 자체는 PMN과 PBMC의 유주성에 직접적인 효과를 보이지 않았다. PMN과 PBMC는 1,2-benzopyrone으로 배양한 PMN의 배양상층액에 대해서도 유주활성을 보이지 않았다. 그러나 1,2-benzopyrone으로 배앙한 PBMC의 배양상층액은 PBMC에 대해서는 유주활성을 보이지 않았으나 PMN에 대해서는 현저한 유주활성을 나타내었다. 또한 유주인자인 IL-8에 의한 PMN의 유주활성 측정 결과도 1,2-benzopyrone으로 배양한 PBMC의 배양상충액의 그것과 유사하였다. 이러한 유주활성은 anti-IL-8 pAb를 처리했을 때 PMN의 유주활성이 억제되어, 본 유주활성은 PBMC에서 분비되는 IL-8일 것으로 강하게 시사되었다. 이상의 결과로부터, 1,2-benzopyrone은 개 말초혈액 PMN의 유주성에 대하여 면역자극 작용을 가지고 있으며, 이것은 1,2-benzopyrone의 자극에 의해 PBMC에서 분비되는 IL-8양 가용성 물질에 의해 나타나는 것으로 사료되었다

Keywords

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