Journal of the Korean Institute of Electrical and Electronic Material Engineers (한국전기전자재료학회논문지)

The Korean Institute of Electrical and Electronic Material Engineers (한국전기전자재료학회)
권호 표지
DOI QR코드

DOI QR Code

Genomic Detection using Electrochemical Method

전기화학적 방법에 의한 유전자의 검출

  • 최용성 (원광대학교 전기전자및정보공학부) ;
  • 이경섭 (동신대학교 전기공학과) ;
  • 박대희 (원광대학교 전기전자및정보공학부)
  • Published : 2005.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

In this paper, a microelectrode away DNA chip was fabricated on glass slide using photolithography technology. Several probe DNAs consisting of mercaptohexyl moiety at their 5' end were immobilized on the gold electrodes by DNA arrayer utilizing the affinity between gold and sulfu. Then target DNAs were hybridized and reacted with Hoechst 33258, which is a DNA minor groove binder and electrochemically active dye. Cyclic voltammetry in 5mA ferricyanide/ferrocyanide solution at 100 mV/s confirmed the immobilization of probe DNA on the gold electrodes. Linear sweep voltammetry or cyclic voltammetry showed a difference between target DNA and control DNA in the anodic peak current values. It was derived from Hoechst 33258 concentrated at the electrode surface through association with formed hybrid. It suggested that this DNA chip could recognize the sequence specific genes. It suggested that multichannel electrochemical DNA microarray is useful to develop a portable device for clinical gene diagnostic system.

Keywords

References

  1. T. Livache, B. Fouque, A. Roget, J. Marchand, G. Bidan, R. Teoule, and G. Mathis, 'DNA chip on a silicon device : example of hepatitis C virus genotyping', Analytical Biochemistry, Vol. 255, p. 188, 1998
  2. D. Y. Guschin, B. K. Mobarry, D. Proudnikov, D. A. Stahl, B. E. Rittmann, and A. D. Mirzabekov, 'Oligonucleotide microchips as genosensors for determinative and environmental studies in microbiology', Appl Environ Microbiol., Vol. 63, No.6, p. 2397, 1997
  3. S. P. A. Fodor, J. L. Read, M. C. Pirrung, L. Stryer, A. T. Lu, and D. Solas, 'Light-directed, spatially addressable parallel chemical synthesis', Science, Vol. 251, p. 767, 1991
  4. S. P. A. Fodor, R. P. Rava, X. C. Juang, A. C. Pease, C. P. Holmes, and C. L. Adams, 'Multiplexed biochemical assays with biological chips', Nature, Vol. 364, p. 555, 1993
  5. M. Schena, D. Shalon, R. W. Davis, and P. O. Brown, 'Quantitative monitoring of gene expression patterns with a complementary DNA microarray' , Science, Vol. 270, p. 467, 1995 https://doi.org/10.1126/science.270.5244.1995
  6. G. Marrazza, I. Chianella, and M. Mascini, 'Disposable DNA electrochemical sensor for hybridization detection', Biosensors & Bioelectronics, Vol. 14, p, 43, 1999
  7. S. O. Kelly, E. M. Boon, J. K. Barton, N. M. Kackson, and M. G. Hill, 'Single-base mismatch detection based on change transduction through DNA', Nucleic Acids Research, Vol. 27, p. 4830, 1999
  8. E. Palecek, M. Fojta, M. Tomschik, and J. Wang, 'Electrochemical biosensors for DNA hybridization and DNA damage', Biosens & Bioelectron, Vol. 13, p. 621, 1998
  9. J. Wang, X. Cai, G. Rivas, H. Shiraishi, P. A. M. Farias, and N. Dontha, 'DNA electrochemical biosensor for the detection of short DNA sequences related to the human immunodeficiency virus', Anal. Chem., Vol. 68, p. 2629, 1996
  10. D. H. Johnson, K. C. Glasgow, and H. H. Thorp, 'Electrochemical measurement of the solvent accessibility of nucleobases using electron transfer between DNA and metal complexes', J. Am. Chem. Soc., Vol. 117, p. 8933, 1995
  11. P. A. Ropp and H. H. Thorp, 'Site-selective electron transfer from purines to electrocatalysts : voltammetric detection of a biologically relevant deletion in hybridized DNA duplexes', Chem Biol., Vol. 6, p. 599, 1999
  12. K. Hashimoto, K. Ito, and Y. Ishimori, 'Sequence-specific gene detection with a gold electrode modified with DNA probes and an electrochemically active dye', Anal. Chem., Vol. 66, p. 3830, 1994
  13. Y. S. Choi, H. K. Shin, Y. S. Kwon, and B. J. Lee, 'Fabrication and organic response characteristics of the copolymer LB films', J. of KIEEME(in Korean), Vol. 9, No.2, p. 180, 1996
  14. 최용성, 권영수, 박대희, '전기화학적 방법에 의한 신규 바이오칩의 SNP 검출', 전기전자재료학회논문지, 17권,4호, p. 410, 2004