Comparison of Bacterial Cellulose Production in a Jar Fermentor Between Acetobacter xylinum BPR2001 and its Mutant, Acetan-Nonproducing Strain EP1

  • BAE SANG OK (Chemical Resources Laboratory, Tokyo Institute of Technology) ;
  • SUGANO YASUSHI (Chemical Resources Laboratory, Tokyo Institute of Technology) ;
  • SHODA MAKOTO (Chemical Resources Laboratory, Tokyo Institute of Technology)
  • Published : 2005.04.01

Abstract

The bacterial cellulose (BC) production by a wild­strain Acetobacter xylinum BPR2001 and that by its acetan­nonproducing mutant, EPI, were compared in a jar fermentor. EPI produced about $28\%$ less BC than the wild-strain. The apparent difference in the cultivation of the two strains was the viscosity increase in the culture broth that was closely associated with acetan production. Increasing the viscosity of the culture broth of EPI by adding agar led to the formation of relatively small and uniform BC pellets, and BC production consequently became two-fold higher than that in the absence of agar and was almost equal to that by BPR2001. Therefore, acetan has an important role in BC production by inducing physical changes in the culture broth of the wild-type strain.

Keywords

References

  1. Tonouchi, N., T. Tsuchida, F. Yoshinaga, T. Beppu, and S. Horinouchi. 1996. Characterization of the biosynthetic pathway of cellulose from glucose and fructose in Acetobacter xylinum. Biosci. Biotechnol. Biochem. 60: 1377-1379 https://doi.org/10.1271/bbb.60.1377
  2. Yoshinaga, F., N. Tonouchi, and K. Watanabe. 1997. Research progress in production of bacterial cellulose by aeration and agitation culture and its application as a new industrial material. Biosci. Biotechnol. Biochem. 61: 219-224 https://doi.org/10.1271/bbb.61.219
  3. Jonas, R. and L. F. Farah. 1998. Production and application of microbial cellulose. Polym. Degrad. Stab. 59: 101- 106 https://doi.org/10.1016/S0141-3910(97)00197-3
  4. Tsuchida, T. and F. Yoshinaga. 1997. Production of bacterial cellulose by agitation culture systems. Pure Appl. Chem. 69: 2453- 2458 https://doi.org/10.1351/pac199769112453
  5. Toyosaki, H., T. Naritomi, A. Seto, M. Matsuoka, T. Tsuchida, and F. Yoshinaga. 1995. Screening of bacterial cellulose-producing Acetohacter strains suitable for agitated culture. Biosci. Biotech. Biochem. 59: 1498-1502 https://doi.org/10.1271/bbb.59.1498
  6. Chao, Y., T. Ishida, Y. Sugano, and M. Shoda. 2000. Bacterial cellulose production by Acetobacter .xylinum in a 50-L internal-loop airlift reactor. Biotech. Bioeng. 68: 345-352 https://doi.org/10.1002/(SICI)1097-0290(20000505)68:3<345::AID-BIT13>3.0.CO;2-M
  7. Son, C. J., S. Y. Chung, J. E. Lee, and S. J. Kim. 2002. Isolation and cultivation characteristics of Acetobacter xylinum KJ-1 producing bacterial cellulose in shaking cultures. J. Microbiol. Biotechnol. 12: 722- 728
  8. Edwards, K. J., A. J. Jay, I. J. Colquhoun, V. J. Morris, M. J. Gasson, and A. M. Griffin. 1999. Generation of a novel polysaccharide by inactivation of the aceP gene from the acetan biosynthetic pathway in Acetobacter xylinum. Microbiology 145: 1499- 1506 https://doi.org/10.1099/13500872-145-6-1499
  9. Ojinnaka, C., A. J. Jay, I. J. Colquhoun, G. J. Brownsey, E. R. Morris, and V. J. Morris. 1996. Structure and conformation of acetan polysaccharide. Int. J. Biol. Macromol. 9: 149-156
  10. Colquhoun, I. J., M. Defernez, and V. J. Morris. 1995. NMR studies of acetan and the related bacterial polysaccharide, CR1/4, produced by a mutant strain of Acetobacter xylinum. Carbohydr. Res. 269: 319- 331 https://doi.org/10.1016/0008-6215(94)00367-O
  11. Wong, H. C., A. L. Fear, R. D. Calhoon, G. H. Eichinger, R. Mayer, D. Amikam, M. Benzirnan, D. H. Gelfand, J. H. Meade, A. W. Emerick, R. Bruner, A. B. Bassat, and R. Tal. 1990. Genetic organization of the cellulose synthase operon in Acetobacter xylinum. Proc. Natl. Acad. Sci. USA 87: 8130- 8134
  12. Couso, R. O., L. Ielpi, and M. A. Dankert. 1987. A xanthangum-like polysaccharide from Acetobacter xylinum. J. Gen. Microbiol. 133: 2123- 2135
  13. Ishida, T., Y. Sugano, and M. Shoda. 2002. Novel glycosyltransferase genes involved in the acetan biosynthesis of Acetobacter xylinum. Biochem. Biophys. Res. Commun. 295: 230- 235 https://doi.org/10.1016/S0006-291X(02)00663-0
  14. Ishida, T., Y. Sugano, T. Nakai, and M. Shoda. 2002. Effect of acetan on production of bacterial cellulose by Acetobacter xylinum. Biosci. Biotech. Biochem. 66: 1677- 1681 https://doi.org/10.1271/bbb.66.1677
  15. Cho, Y. S., R. D. Park, Y. W. Kim, H. Hwangbo, W. J. Jung, J. S. Shu, B. S. Koo, H. B. Krishnan, and K.Y. Kim. 2003. PQQ-dependent organic acid production and effect on common bean growth by Rhizobium tropici CIAT 899. J. Microbiol. Biotechnol. 13: 955- 959
  16. Siegmund, D. and H. Diekmann. 1989. Estimation of fermentation biomass concentration by measuring oxygen uptake off-line with an oxygen electrode. Appl. Microbiol. Biotechnol. 32: 32- 36
  17. Matsuoka, M., T. Tsuchida, K. Matsushita, O. Adachi, and F. Yoshinaga. 1996. A synthetic medium for bacterial cellulose production by Acetobacter xylinum subsp. sucrofermentans. Biosci. Biotech. Biochem. 60: 575- 579 https://doi.org/10.1271/bbb.60.575
  18. Ross, P., R. Mayer, and M. Benziman. 1991. Cellulose biosynthesis and function in bacteria. Microbiol. Rev. 55: 35-58
  19. Brede, G., E. Fjaervik, and S. Valla. 1991. Nucleotide sequence and expression analysis of the Acetobacter xylinum uridine diphosphoglucose pyrophosphorylase gene. J. Bacteriol. 173: 7042- 7045 https://doi.org/10.1128/jb.173.21.7042-7045.1991
  20. Wulf, P. D., K. Joris, and E. J. Vandamme. 1996. Improved cellulose formation by an Acetobacter xylinum mutant limited in (keto)gluconate synthesis. J. Chem. Tech. Biotechnol. 67: 376- 380 https://doi.org/10.1002/(SICI)1097-4660(199612)67:4<376::AID-JCTB569>3.0.CO;2-J
  21. Lee, J. H., J. H. Kim, M. R. Kim, S. M. Lim, S. W. Nam, J. W. Lee, and S. K. Kim. 2002. Effect of dissolved oxygen concentration and pH on the mass production of high molecular weight pullulan by Aureobasidium pullulans. J. Microbiol. Biotechnol. 12: 1-7
  22. Ishikawa, A., T. Tsuchida, and F. Yoshinaga. 1998. Relationship between sulfaguanidine resistance and increased cellulose production in Acetobaeter xylinum BPR 3001E. Biosci. Biotech. Biochem. 62: 1234- 1236 https://doi.org/10.1271/bbb.62.1234
  23. Hong, S. H., S. Y. Moon, and S. Y. Lee. 2003. Prediction of maximum yields of metabolites and optimal pathways for their production by metabolic flux analysis. J. Microbiol. Biotechnol. 13: 571- 577