Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Molecular Characterization of TEM-type $\beta$-Lactamases Identified in Cold-Seep Sediments of Edison Seamount (South of Lihir Island, Papua New Guinea)

  • Song Jae Seok (Department of Biological Sciences, Myongji University) ;
  • Jeon Jeong Ho (Marine Biotechnology Center, Korea Ocean Research & Development Institute) ;
  • Lee Jung Hun (Department of Biological Sciences, Myongji University, Marine Biotechnology Center, Korea Ocean Research & Development Institute) ;
  • Jeong Seok Hoon (Department of Laboratory Medicine, Kosin University College of Medicine) ;
  • Jeong Byeong Chul (Department of Biological Sciences, Myongji University) ;
  • Kim Sang Jin (Marine Biotechnology Center, Korea Ocean Research & Development Institute) ;
  • Lee Jung Hyun (Marine Biotechnology Center, Korea Ocean Research & Development Institute) ;
  • Lee Sang Hee (Department of Biological Sciences, Myongji University)
  • Published : 2005.04.01
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Abstract

To determine the prevalence and genotypes of $\beta$-lactamases among clones of a metagenomic library from the cold-seep sediments of Edison seamount (10,000 years old), we performed pulse-field gel electrophoresis, antibiotic susceptibility testing, pI determination, and DNA sequencing analysis. Among the 8,823 clones of the library, thirty clones produced $\beta$-lactamases and had high levels of genetic diversity. Consistent with minimum inhibitory concentration patterns, we found that five ($167\%$) of thirty clones produced an extended-spectrum $\beta$-lactamase. 837- and 259-bp fragments specific to bla$_{TEM}$ genes were amplified, as determined by banding patterns of PCR amplification with designed primers. TEM­1 was the most prevalent $\beta$-lactamase and conferred resistance to ampicillin, piperacillin, and cephalothin. TEM-116 had a spectrum that was extended to ceftazidime, cefotaxime, and aztreonam. The resistance levels conferred by the pre-antibiotic era alleles of TEM-type $\beta$-lactamases were essentially the same as the resistance levels conferred by the TEM-type alleles which had been isolated from clinically resistant strains of bacteria of the antibiotic era. Our first report on TEM-type $\beta$-lactamases of the pre-antibiotic era indicates that TEM-type $\beta$-lactamases paint a picture in which most of the diversity of the enzymes may not be the result of recent evolution, but that of ancient evolution.

Keywords

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