Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Growth and DNA Alteration of Heavy-ion Beam Irradiated Tobacco(Nicotiana plumbaginifolia) Plant

중이온 빔조사 담배(Nicotiana plumbaginifolia) 식물체의 생장과 DNA 변이

  • Lyu Jae-Il (Department of Plant Production Sciences, College of Agriculture and Life Sciences, Sunchon National University) ;
  • Kim Min-Su (Department of Plant Production Sciences, College of Agriculture and Life Sciences, Sunchon National University) ;
  • Tomoko Abe (Discovery Research Institute, The Institute of Physical and Chemical Research) ;
  • Lee Hyo-Yeon (Faculty of Biotechnology, College of Applied Life Sciences, Cheju National University) ;
  • Yang Deok-Chun (College of Life Science & Center for Oriental Medicinal Materials and Processing, Kyung Hee University) ;
  • Bae Chang-Hyu (Department of Plant Production Sciences, College of Agriculture and Life Sciences, Sunchon National University)
  • 류재일 (순천대학교 농업생명과학대학 식물생산과학부) ;
  • 김민수 (순천대학교 농업생명과학대학 식물생산과학부) ;
  • ;
  • 이효연 (제주대학교 생명자원과학대학 생명공학부) ;
  • 양덕춘 (경희대학교 한방재료가공센터) ;
  • 배창휴 (순천대학교 농업생명과학대학 식물생산과학부)
  • Published : 2005.02.01
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Abstract

Effects of heavy-ion beam$(^{20}Ne)$ irradiation on growth and DNA alteration of tobacco plants were investigated. Seed germination and plant height were decresed as the ion-beam intensity was increased. However, the bolting and flowering were promoted by the low intensities of 5 Gy to 10 Gy treatment. Out of the 100 primers screened, 59 primers generated 336 DNA fragments by RAPD analysis, and one specific DNA fragment that amplified in control but not in the ion-beam irradiated plants was observed. By AFLP analysis, DNA fragment difference related to the ion-beam treatment was not detected but observed among the plant bodys.

중이온빔$(^{20}Ne)$을 담배 식물체에 조사(irradiation)하여 식물체의 생장과 DNA의 변이에 미치는 영향을 검토하였다. 발아율과 초장은 조사선 량이 증가함에 따라 감소하였다. 반면 $50Gy\~10Gy$의 저선량에서 추대와 개화가 촉진되어 발육은 오히려 촉진되었다. 총 100개의 primer를 이용하여 RAPD분석한 결과, 59개의 primer에서 총 336개의 DNA단편이 증폭되었고, 1개의 primer에서 중이온빔 조사처리구에서만 출현하는 DNA단편이 나타났다. AFLP분석 결과, 개체 특이적인 DNA단편은 나타난 반면 중이온빔 처리구 특이적인 DNA 단편은 관찰되지 않았다.

Keywords

References

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