Production of Intracelluar Tyrosinase Inhibitor from Malassezia pachydermatis

Malassezia pachydermatis에 의한 세포 내 Tyrosinase 저해제의 생산

  • Lee, Sung-Hyun (Dept. of Genetic Engineering and Bio-medical Regional Research Center, Paichai University) ;
  • Yu, Hyung-Eun (Dept. of Genetic Engineering and Bio-medical Regional Research Center, Paichai University) ;
  • Kwak, Yoon-Jin (Dept. of Genetic Engineering and Bio-medical Regional Research Center, Paichai University) ;
  • Kim, Hyo-Jin (Dept. of Genetic Engineering and Bio-medical Regional Research Center, Paichai University) ;
  • Lee, Dae-Hyoung (Dept. of Genetic Engineering and Bio-medical Regional Research Center, Paichai University) ;
  • Lee, Jong-Soo (Dept. of Genetic Engineering and Bio-medical Regional Research Center, Paichai University)
  • 이성현 (배재대학교 유전공학과.바이오의약 연구센터) ;
  • 유형은 (배재대학교 유전공학과.바이오의약 연구센터) ;
  • 곽윤진 (배재대학교 유전공학과.바이오의약 연구센터) ;
  • 김효진 (배재대학교 유전공학과.바이오의약 연구센터) ;
  • 이대형 (배재대학교 유전공학과.바이오의약 연구센터) ;
  • 이종수 (배재대학교 유전공학과.바이오의약 연구센터)
  • Published : 2004.08.30

Abstract

A yeast strain SL-27 found to produce active intracellular tyrosinase inhibitor was screened from 972 kinds of yeasts. It was identified as Malassezia pachydermatis based on microbiological characteristics. The optimum pH and temperature for the growth of Malassezia pachydermatis SL-27 were pH 7.0 and $37^{\circ}C$, respectively. The optimal culture conditions for the production of tyrosinase inhibitor by Malassezia pachydermatis SL-27 were investigated. The optimal medium cimposition for tyrosinase inhibitor production was determined to be 1.0% casamino acid, 2.0% glucose, 0.1% $KH_2PO_4$, 0.05% $MgSo_{4-}7H20$ and each 0.01 of $CaCl_2$ and NaCl. Optimal initial pH and temperature for the production of tyrosinase inhibitor were pH 5.0 and $30^{\circ}C$, respectively. The maximum tyrosinase inhibitory activity of 84%/mL of cell-free extract was showed after 12 h of cultivation under the optimal culturing conditions.

Keywords