흰쥐 해마의 치상회에서 압박자극 적용이 뇌 신경세포 증식에 미치는 영향

Effects of Compression Stimulation Application on Cell Proliferation in the Hippocampal Dentate Gyrus of the Sprague-Dawley Rats

  • 유병규 (신구대학 보건의료학부 물리치료과) ;
  • 김경미 (인제대학교 의생명공학대학 작업치료학과) ;
  • 김창주 (경희대학교 의과대학 생리학교실)
  • Yu, Byong-Kyu (Department of Physical Therapy, Shingu College) ;
  • Kim, Kyeong-Mi (Department of Occupational Therapy, College of Biomedical Science and Engineering, Inje University) ;
  • Kim, Chang-Ju (Department of Physiology, College of Medicine, Kyung Hee University)
  • 발행 : 2004.09.10

초록

Objective : Effect of treadmill exercise on hippocampal neural cell proliferation under normal conditions and alcohol intoxication conditions has been recently studied; however, this effect under sensory stimulation application has not clarified yet. In the present study, the effect of compression stimulation application on hippocampal neural cell proliferation in the dentate gyrus in normal and alcohol intoxicated rats was investigated. Methods : Experimental design: comparative investigation on number of 5-Bromo-2'-deoxyuridine(BrdU)B-positive cells in dentate gyrus 5 days after commencement. Setting: animal laboratory. Participants: male Sprague-Dawley rats of 3weeks old in age weighing $80{\pm}10gm$. Intervention: animals were randomly assigned into 4 groups; control-rest group(n=8), control-compression group(n=8), alcohol intoxication-rest group(n=8) and alcohol intoxication-compression group(n=8). Animals of the alcohol intoxicated groups were injected intraperitoneally with alcohol(2g/kg) twice per day for 3 days. All animals were injected BrdU(50mg/kg) intraperitoneally, and rats compression stimulation application groups were compressed using sphygmomanometer cuff times per day, for 5 days following alcohol administration. Measures: mean number of BrdU-positive cells in dentate gyrus was observed via immunohistochemistry. Results : Compression stimulation application significantly increased the number of BrdU-positive cells in the dentate gyrus. Also, treatment with alcohol for 3 days inhibited cell proliferation, and compression stimulation application alleviated alcohol-induced inhibition of new cell formation. Conclusion : These results suggest the possibility that compression stimulation application may help in improvement following alcohol-induced brain damaged.

키워드