Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
권호 표지

Evaluation of Dry Rehydratable Film Method for Enumeration of Microorganisms in Korean Traditional Foods

한국 전통식품 중 미생물 분석을 위한 건조필름법 평가

  • 김관식 (연세대학교 생명공학과) ;
  • 배은경 (연세대학교 생명공학과) ;
  • 하상도 (중앙대학교 식품공학과) ;
  • 박영서 (경원대학교 식품생물공학과) ;
  • 목철균 (경원대학교 식품생물공학과) ;
  • 홍관표 (한국쓰리엠주식회사) ;
  • 김상필 (한국쓰리엠주식회사) ;
  • 박지용 (연세대학교 생명공학과)
  • Published : 2004.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

Dry rehydratable film methods were compared to conventional methods for the enumeration of microorganisms in Korean traditional foods. Kimchi, doenjang, kochujang, kanjang, takju, sujeongkwa and sikhe were used as Korean traditional foods. $Petrifilm^{TM}$ aerobic count plate, $Petrifilm^{TM}$ coliform count plate, $Petrifilm^{TM}$ E. coli/coliform count plate, $Petrifilm^{TM}$ yeast and mold count plate and $Petrifilm^{TM}$ staph express count plate were compared to plate count agar, most probable number (MPN) for coliform, MPN for E. coli, potato dextrose agar and coagulase test, respectively. Regression analysis indicated that correlation coefficient values were 0.974-0.998, 0.913-0.995, 0.955-0.978, 0.968-0.986 and 0.998-0.999 for total aerobic bacteria, yeast and mold, coliform, E. coli and S. aureus, respectively. There were no significant differences between two methods, suggesting that $Petrifilm^{TM}$ plates can be used as an alternative to conventional method for the determination of microorganisms in Korean traditional foods.

우리나라 전통식품인 김치, 된장, 고추장, 간장. 탁주, 식혜, 수정과에 있는 미생물 분석을 위하여 건조필름법과 전통적인 미생물 분석법을 비교하였다. 일반세균 분석에는 plate count agar 법과 $Petrifilm^{TM}$ aerobic count plate 법을 비교하였고, 대장균군과 대장균의 분석에는 most probable number (MPN) 법과 $Petrifilm^{TM}$ coliform count plate 및 $Petrifilm^{TM}$ E. coli/colmfom count plate 법을 각각 비교하였으며, 효모와 곰팡이의 분석에는 potato dextrose agar 법과 $Petrifilm^{TM}$ yeast and mold count plate 법을 비교하였다. 황색포도상구균의 분석에는 coagulase 시험법과 $Petrifilm^{TM}$ Staph express fount plate 법을 비교하였다. 두 방법간의 상관계수는 일반세균이 0.974-0.998, 대장균군이 0.955-0.978, 대장균이 0.968-0.986, 효모와 곰팡이가 0.913-0.995, 황색포도상구균이 0.998-0.999로 두 방법간의 상관성이 매우 높았으며, 평균 미생물수에 있어서도 유의적인 차이가 없었다(p>0.05). 이러한 결과로부터 건조필름법이 기존의 전통적인 방법을 대체할 수 있는 미생물 분석법임이 확인되었다.

Keywords

References

  1. International Commission on Microbiological Specifications for Foods (ICMSF) Microorganisms in Foods. 1. Their Significance and Methods of Enumeration, 2nd ed., University of Toronto Press, Toronto, Ontario (1986)
  2. Hitchens A.D., Feng P., Watkins W.D., Rippey S.R. and Chandler L.A: Escherichia coli and the coliform bacteria. Bacteriological Analytical Manual, 8th ed. AOAC International, Craithersburg, M.D. 64, 669-673 (1995)
  3. Anderson J.M. and Baird-Parker A.C.: A rapid and direct plate method for enumerating Escherichia coli biotype I in food. J. Appl. Bacteriol. 39, 111-117 (1975). https://doi.org/10.1111/j.1365-2672.1975.tb00551.x
  4. Oblinger J.L. and Koburger J.A.: Understanding and teaching the most probable number technique. J. Milk Food Technol. 38, 540-545 (1975)
  5. Oblinger J.L. and Koburger J.A.: The most probable number technique, pp. 99-111. Compendium of Methods for the Microbiological Examination of Food, 2nd ed., American Public Health Association, Washington, DC (1984)
  6. Feng P.C.S. and Hartman P.A.: Fluorogenic assays for mmediate confmnation of Escherichia coli. Appl. Environ. Microbiol. 43, 1320-1329 (1982)
  7. Firstenberg-Eden R. Electrical impedance method for determining microbial quality of foods, pp. 679-687. Rapid Methods and Automation in Microbiology and Immunology. Hobermehl, K.O. (ed.), Springer-Verlag, Berlin (1985)
  8. Anonymous, Australian Standard 1095. Microbiological Methods for the Dairy Industry. Standard Association of Australia, Sydney, Australia (1987).
  9. King A.D., Hocking A.D., and Pitt J.I.: Dichloran-rose bengal medium for enumeration and isolation of molds from foods. Appl. Environ. Microbiol. 37, 959-964 (1979)
  10. Mossel D.A.A., Kleynen-Semme1ing A.M.C., Vincentie H.M., Beerens H., and Catsaras M.: Oxytetracyclineglucoseyeast extract agar for selective enumeration of moulds and yeasts in foods or clinical materials. J. Appl. Bacteriol. 33, 454-457 (1970) https://doi.org/10.1111/j.1365-2672.1970.tb02220.x
  11. MacLaren J.A. and Armen D.: Pigmentation of Candida albicans by molybdenum. Arner. J. Clin. Pathol. 30, 411-422 (1958)
  12. Bennett R.W. and Lancette G.A: Staphylococcus aureus. pp. 12.01-12.05. In FDA Bacteriological Analytical Manual 8th ed. (revision A). Jackson G.J. (ed.), Gaithersburg, Maryland, USA, AOAC International (1998)
  13. De-Buyser M.L., Audinet N., Delbart M.O., Maire M., and Francoise F.: Comparison of selective culture media to enumerate coagulase-positive Staphylococci in cheeses made from raw milk. Food Microbiol. 15, 339-346 (1998) https://doi.org/10.1006/fmic.1997.0176
  14. Wilson I.G, Gilmour A, Cooper J.E., Bjourson A.J., and Harvey J.: A non-isotopic DNA hybridization assay for the identification of Staphylococcus aureus isolated from foods. Int. J. Food Microbiol. 22, 43-54 (1994) https://doi.org/10.1016/0168-1605(94)90006-X
  15. MaAllister J.S., Ramos M.S., and Fox T.L.: Evaluation of the 3M dry medium culture plate (Petrifilm™ 3M) method for enumerating bacteria in processed fluid milk samples. Dairy and Food Sanitation 7, 632-635 (1987)
  16. Ginn R.G, Packard V.S., and Fox T.L.: Enumeration of total bacteria and coliforms in milk by dry rehydratable film methods: Collaborative study. J. Assoc. Off. Anal. Chem. 69, 527-531 (1986)
  17. Ha S.D.: Evaluation of dry film method for isolation of microorganisms from foods. Kor. J. Appl. Microbiol. Biotechnol. 24(2), 178-184 (1996)
  18. KFDA Code of Food Law, Korea Food and Drug Administration (2000)
  19. Beloti V., Barros M.A.E, Nero L.A., Pachemshy J.A.S., Santana E.H.W., and Franco B.D.G.M.: Quality of pasteurized milk influences the performance of ready-to-use systems for enumeration of aerobic microorganisms. Intl. Dairy J. 12, 413-418 (2002) https://doi.org/10.1016/S0958-6946(02)00021-3
  20. Nelson C.L., Fox T.L., and Busta F.F: Evaluatin of dry medium film (petrifilm™ VBR) for coliform enumeration. J. Food Prot. 47, 520-525 (1984)
  21. Park Y.H., Seo K.S., Aliri J.S., Yoo H.S., and Kim S.P.: Evaluation of the Petrifilm$^T^M$ plate method for the enumeration of aerobic microorganisms and coliform in retailed meat samples. J. Food Prot. 64(11), 1841-1843 (2001)
  22. Suwansonthichai S. and Rengpipat S.: Enumeration of coliforms and Escherichia coli in frozen black tiger shrimp Penaeus monodon by conventional and rapid methods. Int. J. Food Microbiol. 81, 113-121 (2003) https://doi.org/10.1016/S0168-1605(02)00190-3
  23. Knight M.T., Newman M.C., Benzinger M.J., Neufang K.L., and Agin J.R.: Comparison of the$Petrifilm^T^M$ dry rehydratable fIlm and conventional culture methods for enumeration of yeasts and molds in foods: Collaborative study. J. AOAC Intl. 80(4), 806-811 (1997)
  24. Schoeller N.P. and Ingham S.c.: Comparison of the BairdParker agar and 3M $Petrifilm^T^M$ rapid S. aureus count plate methods for detection and enumeration of Staphylococcus aureus. Food Microbiol. 18, 581-587 (2001) https://doi.org/10.1006/fmic.2001.0432