한국작물학회지 (KOREAN JOURNAL OF CROP SCIENCE)

  • 제49권5호
  • /
  • Pages.415-418
  • /
  • 2004
  • /
  • 0252-9777(pISSN)
  • /
  • 2287-8432(eISSN)
한국작물학회 (The Korean Society of Crop Science)
권호 표지

Effects of Sonication and Vacuum Infiltration on Agrobacterium-Mediated Transformation in Immature Embryos of Korean Wheat Genotypes

  • 발행 : 2004.12.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The effects of sonication and vacuum infiltration on transformation efficiency was investigated by using immature embryos of Korean wheat as explants. Two Agrobacterium tumefaciens strains, KYRT1 and EHA105, carrying pCAMBIA 1305.1 were used. Transformation efficiency was demonstrated by the detection of $\beta-glucu-ronidase$ (GUS) activity. GUS expression showed clear difference among Korean wheat cultivars. Geurumil showed higher GUS expression efficiency $79.1\%$ compared with other cultivars. The effects of the duration of vacuum infiltration and sonication treatment showed a tendency high GUS expression efficiency by their combination. In comparison with other Agrobacterium strains, KYRT1 showed high efficiency in most Korean cultivars.

키워드

참고문헌

  1. Amoah, B. K., H Wu, C. Sparks, and H. D. Jones 2001 Factors influencing Agrobacterium-mediated transient expression of uidA in wheat inflorescence tissue. Journal of Experimental Botany 52. 1135-1142 https://doi.org/10.1093/jexbot/52.358.1135
  2. Chang, S. S., S. K Park, B. C. Kim, B J. Kang, and D. U. Kim. 1994 Stable genetic transformation of Arabidopsis thaliana by Agrobacterium inoculation in planta The Plant J 5 : 551-558 https://doi.org/10.1046/j.1365-313X.1994.5040551.x
  3. Cheng, M, J. E. Fry, S. Pang, H. Zhou, C, M. Hironaka, D R. Duncan, T W. Conner, Y. Wan 1997 Genetic transformation of wheat mediated by Agrobacterium tumefaciens Plant Physiol. 115 : 971-980 https://doi.org/10.1104/pp.115.3.971
  4. Clough, S. J. and A. F. Bent. 1998 Floral dip. a Simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. The Plant J. 16 : 735-743 https://doi.org/10.1046/j.1365-313x.1998.00343.x
  5. Hood, E. E., S. B. Gelvin, L. S. Melchers, and A. Hoekema. 1993. New Agrobacterium helper plasmids for gene transfer to plants Transgenic Res. 2 208-218 https://doi.org/10.1007/BF01977351
  6. Jefferson, R A 1987. Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol. BioI Rep. 5 : 387-405 https://doi.org/10.1007/BF02667740
  7. Joersbo, M. and J. Brunstedt 1990. Direct gene transfer to plant protoplasts by mild sonication Plant Cell Rep. 9 : 207-210
  8. Joersbo, M. and J. Brunstedt. 1992 Sonication. A new method for gene transfer to plants. Physiology Plantarum 85:230-234 https://doi.org/10.1111/j.1399-3054.1992.tb04727.x
  9. Lee, B. M. 2001. Effect of sonication and vir genes on transient gene expression in Agrobacterium-mediated transformation. Korean J of LIfe Sci 11 : 316-320
  10. Mysore, K. S., C T R. Kumar, and S. B. Gelvin. 2000. Arabidopsis ecotypes and mutants that are recalcitrant to Agrobacterium root transformation are susceptible to germ-line transformation The Plant J. 21 : 9-16 https://doi.org/10.1046/j.1365-313x.2000.00646.x
  11. Tingay, S., D. McElroy, R Kalla, S. Fieg, M. B. Wang, S. Thornton, and R. Brettell. 1997. Agrobacterium tumefaciens-mediated barley transformation The Plant J 11 . 1369-1376 https://doi.org/10.1046/j.1365-313X.1997.11061369.x
  12. Trick, H N and J. J Finer. 1997. SAAT: sonication-assisted Agrobacterium- mediated transformation Transgenic Res. 6 . 329-336 https://doi.org/10.1023/A:1018470930944
  13. Trick, H N and J. J. Finer. 1998 Sonication-assited Agrobacterium- mediated transformation of soybean 'Glycine max (L.) Merrill' embryonic suspension culture tissue Plant Cell Rep. 17 : 482-488 https://doi.org/10.1007/s002990050429
  14. Trick, H N. and J J Finer. 1999 Induction of Somatic embryogenesis and genetic transformation of Ohio buckeye (Aesculus glabra Willd.). In Vitro Cell Dev. BioI. 35 : 57-60 https://doi.org/10.1007/s11627-999-0010-4
  15. Torisky, R. S., L. Kovacs, S. Avdiushko, J. D. Newman, A. G. Hunt, and G. B. Collins 1997. Development of a binary vector system for plant transformation based on the supervirulent Agrobacterium tumefaciens strain Chry5 Plant Cell Rep. 17. 102-108 https://doi.org/10.1007/s002990050360
  16. Wang, M , D. C. Abbott, N. M Upadhyaya, J. V. Jacobsen, and P M. Waterhouse 2001. Agrobacterium tumefaciens-mediated transformation of an elite Australian barley cultivar with virus resistance and reporter genes Aust. J. Plant Physiol. 28 . 149-156
  17. Weir, B., X. Gu, M. B. Wang, N Upadhyaya, A. R. Elliott, and R. I S. Brettell. 2001 Agrobacterium tumefaciens-mediated transformation of wheat using suspension cells as a model system and green fluorescent protein as a visual marker Aust J. Plant Physiol, 28 807-818
  18. Wu, H., A C. McCormac, M. C. Elliott, and D. F. Chen 1998 Agrobacterium-mediated stable transformation of suspension cultures of barley (Hordeum vulgare L.). Plant Cell Tissue Organ Cult. 54 : 161-167 https://doi.org/10.1023/A:1006106916542
  19. Wu, H., C. Sparks, B. Amoah, H. D Jones. 2003 Factors influencing successful Agrobacterium-mediated genetic transformation of wheat. Plant Cell Rep 21 : 695-668
  20. Ye, G N , D Stone, S. Z Pang, W. Creely, K. Gonzalez, and M. Hinchee. 1999. Arabidopsis ovule is the target for Agrobacterium in planta vacuum infiltration transformation The Plant J 19 . 249-257 https://doi.org/10.1046/j.1365-313X.1999.00520.x