KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
권호 표지

Development of AFLP and STS Markers Related to Stay Green Trait in Multi-Tillered Maize

  • Jang Cheol Seong (Laboratory of Plant Molecular breeding, Division of Biotechnology and Genetic Engineering, College of life and Environmental Science, Korea University) ;
  • Lee Hee Bong (Department of Agronomy, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Seo Yong Weon (Laboratory of Plant Molecular breeding, Division of Biotechnology and Genetic Engineering, College of life and Environmental Science, Korea University)
  • Published : 2004.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

In order to develop molecular markers related to stay green phenotype, AFLP analysis was conducted using near-isogenic lines for either stay green or non stay green trait. Both lines have characteristics of multi-ear and tillers (MET). Two out of 64 primer combinations of selective amplification identified three reproducible polymorphic fragments in MET corn with stay green. Both of E+AGC/M+CAC and E+AAG/M+CAA primer combinations produced two and one specific polymorphic fragments linked to stay green trait, respectively. For the conversion of AFLPs to sequence tag sites (STSs), primers were designed form both end sequences of each two polymorphic fragments. One fragment, which was amplified with E+AAG/M+CAA primer combinations, possessed 298 bp long and showed a $91\%$ homology with maize retrotransposon Cinful-l. One out of two polymorphic fragments produced with E+AGC/M+CAC primer combination had 236 bp long and matched a $96\%$ homology with an intron region of 22kDa alpha zein gene cluster in Zea mays. One out of two PCR fragments amplified with MET2 primer set in the stay green MET was not produced in the non-stay green MET. The developed AFLP and STS marker could be used as an efficient tool for selection of the stay green trait in the MET inbred.

Keywords

References

  1. Cha, K. -W, Y. J. Lee, H. -J. Koh, B. -M.Lee, Y. -W Nam, and N. -C. Paek. 2002.Isolation, characterization, and mapping of the stay green mutatnt in rice. Theor Appl.Genet. 104 526-532 https://doi.org/10.1007/s001220100750
  2. Cheo, B. H., H. B Lee, and W K. Lee 1991. Agronomic characteristics of maize inbreds with tillers and their hybrids Korean J. Breed.23 265-268
  3. Cheo,B H, H B Lee,W K Lee,H C. Ji,C. T. Chung,and S.K Jong. 1994 Characteristics of tillering maize inbred IK4. Korean J. Breed 26 447-450
  4. Gentinetta E, D. Ceppi, C. Lpon, G Perico, M Motto, and F. Salamini 1986. A major gene for delayed senescence in maize Pattern of photosynthates accumulation and inheritance. Plant Breed.97 193-203 https://doi.org/10.1111/j.1439-0523.1986.tb01053.x
  5. Haussmann, B. I. G, V. Mahalakshmi, B V .S. Reddy, N. Seetharama, C. T.Hash,and H. H. Geiger 2002 QTL mapping of stay green In two sorghum recombinant inbred populatons. Theor. Appl. Genet 106: 133-142 https://doi.org/10.1007/s00122-002-1012-3
  6. JiangG H, Y Q He,C G Xu, X.H Li,andQ Zhang 2004 The genetic basis of stay-green in rice analyzed in a population of double haploid lines derived form an indica by japonica cross. Theor. Appl. Genet 108. 688-698 https://doi.org/10.1007/s00122-003-1465-z
  7. Jang, C. S., H. B. Lee, and Y. W Seo. 2004. Molecular characterization of two genes,ZmRPS4 and ZmMET1,expressed In the crowns of tillers in maize Maydica(accepted)
  8. Kim, D S, I S. Lee,C S Jang, S Y. Kang,H. S. Song,Y I. Lee, andY W Seo.2004 Development of AFLP-derived STS makers for the selection of 5- methyltryptophan resistant rice mutants Plant Cell Rep. 23 . 71-80
  9. Luquez, V.M and J. J. Guiamet 2002.The stay green mutants dl and d2 increase water stress susceptibihty in soybeans. J Exp Bot. 53.1421-1428 https://doi.org/10.1093/jexbot/53.373.1421
  10. Major D. J 1977 Seasonal dry-weight distribution of singlestalked and multi-tillered corn hybrids grown at three population densities in southern Alberta. Can J Plant Sci 57 . 1041-1047 https://doi.org/10.4141/cjps77-155
  11. Qu, L-J , T. N. Foote, M A. Roberts, T A. Money, L Aragon-Alcaide, J W. Snape, and G Moore 1998 A simple PCR-based method for scoring the phlb deletion in wheat Theor. Appl Genet.96 371 -375 https://doi.org/10.1007/s001220050751
  12. Seo, Y W, C S Jang, and J W Johnson. 2001 Development of AFLP and STS markers for identifying wheat-rye translocations possessing2RL. Euphytica 121 . 279-287 https://doi.org/10.1023/A:1012020532485
  13. Shan, X., T K Blake, and L. E. Talbert. 1999 Conversion of AFLP markers to sequence-specific PCR markers in barley and wheat Theor Appl Genet 98 . 1072-1078 https://doi.org/10.1007/s001220051169
  14. Spano, G., N. D Fonzo, C Perrotta, C Platani, G. Ronga, D W Lowlor, J. A Napier, and P R Shewry 2003 Physiological characterization of stay green mutants in durum wheat J. Exp Bot. 54 1415-1420 https://doi.org/10.1093/jxb/erg150
  15. Thomas H., C Evans, H M Thomas, M W. Humphreys, G Moran, B. Hauck, and I. Donnison 1997. Introgression, tag. ging and expression of a leaf senescence gene in Festulolium New Phytol. 137 . 29-34 https://doi.org/10.1046/j.1469-8137.1997.00830.x
  16. Thomas H. and C. J. Howarth. 2000. Five ways to stay green J Exp Bot. 51 : 329-337 https://doi.org/10.1093/jexbot/51.suppl_1.329
  17. Vos, P., R. Hangers, M. Bleeker, M. Reijians, T. van de Lee, M Hornes. A Frijiters, J. Pot, J. Peleman, M Kuiper, and M Zabeau. 1995. AFLP a new technique for DNA fingerprinting Nucleic Acids Res 23.4404-4414