Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
권호 표지

Isolation of Angiotensin I-Converting Enzyme Inhibitory Peptide from Chungkookjang

청국장으로부터 Angiotensin I 전환효소 저해 Peptide의 분리

  • Matsui Toshiro (Bioscience and Biotechnology, Graduate School of Agriculture, Kyushu University) ;
  • Yoo Hyung Jae (Department of Life Science, Hoseo University) ;
  • Hwang Jae Sung (Department of Life Science, Hoseo University) ;
  • Lee Dong Seok (Department of Medical Laboratory Science, Inje University) ;
  • Kim Han Bok (Department of Life Science, Hoseo University)
  • 송정이랑 (일본 구주대학원 농학연구원) ;
  • 유형재 (호서대학교 자연과학부 생명과학전공) ;
  • 황재성 (호서대학교 자연과학부 생명과학전공) ;
  • 이동석 (인제대학교 의생명공학대학 임상병리학과) ;
  • 김한복 (호서대학교 자연과학부 생명과학전공)
  • Published : 2004.12.01
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Abstract

Chunkookjang, Korean traditional fermented soybean food emerges as a functional food to improve intestinal function and blood circulation. During Chungkookjang fermentation, microorganisms, enzymes, and diverse bioactive compounds increase sharply. Chungkookjang contains diverse oligo-peptides. Formation of peptides was confirmed by SDS-PAGE. Solube fermented soybean in our sample contained Tyr, Gln-Lys, Trp, Gln, and Lys-Pro as major components. Lys-Pro (0.083 mg/100 g sample) was purified by HPLC analysis. Angiotensin I­converting enzyme (ACE) causes hypertension by converting angiotensin I to angiotension II. ACE inhibitory activity of Lys-Pro was determined to be $IC_{50}=32.1\;{\mu}M.$ Whether or not eating Chungkookjang can lower blood pressure was also determined. Sistolic blood pressure dropped by 15 mmHg, and diastolic blood pressure by 8 mmHg 2 hr after a single administration of 20 g of fermented soybean. Chungkookjang might be helpful in improving blood circulation since it has ACE inhibitor and antihypertenisve effect.

한국의 전통 대두 발효식품 청국장이 정장, 혈액순환 개선 둥의 기능성식품으로 부각되고 있다. 청국장은 발효가 되면서 미생물, 효소, 다양한 생리활성물질이 크게 증가하며, 그 중에는 peptide류도 포함된다. 청국장 Peptide류의 형성은 SDS-PAGE 분석을 통해 확인할 수 있었다. 청국장의 수용성 아미노산중, Tyr, Gln-Lys, Trp, Gln, Lys-Pro 등이 주요성분으로 발견되었고, Lys-Pro (0.083 mg/100 g 시료)가 HPLC에 의해 분리되었다. Lys-Pro은 angiotensin I-converting enzyme (ACE)저해효과 $(IC_{50}=32.1{\mu}m)$를 지니고 있었다. 고혈압군이 분말청국장 20 g을 복용하고 2시간 지났을 때, 수축기 혈압은 15 mmHg, 이완기 혈압은 8 mmHg떨어지는 강하효과가 있었다. 청국장은 ACE 저해제와 혈압강하효과를 갖고 있기 때문에 혈액순환개선에 상당한 도움을 주는 기능성 식품으로 개발될 수 있을 것이다.

Keywords

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