KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Sp1 Decoy Oligodeoxynucleotides Inhibit Serum-induced Mesangial Cell Proliferation

Sp1 Decoy Oligodeoxynucleotides에 의한 사구체 혈관간세포 증식억제 효과

  • Chae Young Mi (Department of Pathology, College of Medicine, Catholic University of Daegu) ;
  • Kim Sung Young (Department of Food Science, Andong Science College) ;
  • Park Kwan Kyu (Department of Pathology, College of Medicine, Catholic University of Daegu) ;
  • Chang Young Chae (Department of Pathology, College of Medicine, Catholic University of Daegu)
  • 채영미 (대구카톨릭대학교 의과대학 병리학교실) ;
  • 김성영 (인동과학대학 식품조리과) ;
  • 박관규 (대구카톨릭대학교 의과대학 병리학교실) ;
  • 장영제 (대구카톨릭대학교 의과대학 병리학교실)
  • Published : 2004.10.01
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Abstract

Mesangial expansion caused by cell proliferation and glomerular extracellular matrix accumulation is one of the earliest renal abnormalties observed at the onset of hyperglycemia in diabetes mellitus. Transcription factor Sp1 is implicated in the transcriptional regulation of a wide range of genes participating in cell proliferation, and is assumed to play an essential role in mesangial expansion, transforming growth factor (TGF)-$\beta$1, plasminogen activator inhibitor (PAI)-1. We have generated a phosphorothioated double-stranded Sp1-decoy oligodeoxynucleotide that effectively blocks Sp1 binding to the promoter region for transcriptional regulation of TGF-$\beta$1 and PAI-1. The Sp1 decoy oligodeoxynucleotide suppressed transcription of these cytokines and proliferation of primary rat mesangial cells in response to serum stimulation. These results suggest that the Sp1 decoy oligodeoxynucleotide could bea powerful tool in preventing the pathogenesis of renal hypertrophy.

Keywords

References

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