Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
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The Protective Effect of Hirudin Herbal-acupuncture against the Neuronal Damage Induced by Middle Cerebral Artery Occulsion(MCAO) in Rats

Hirudin 약침(藥鍼)이 중대뇌동맥폐색(中大腦動脈閉塞)으로 유발(誘發)된 흰쥐의 신경손상(神經損傷) 보호(保護) 효과(效果)

  • Suk, Jae-wook (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Hanny University) ;
  • Jung, Tae-young (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Hanny University) ;
  • Lim, Seong-cheol (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Hanny University) ;
  • Seo, Jeong-chul (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Hanny University) ;
  • Kim, Mi-ryeo (Department of Pharmacology, College of Oriental Medicine, Daegu Hanny University) ;
  • Yang, Chae-ha (Department of Physiology, College of Oriental Medicine, Daegu Hanny University) ;
  • Han, Sang-won (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Hanny University)
  • 석재욱 (대구한의대학교 한의과대학 침구학교실) ;
  • 정태영 (대구한의대학교 한의과대학 침구학교실) ;
  • 임성철 (대구한의대학교 한의과대학 침구학교실) ;
  • 서정철 (대구한의대학교 한의과대학 침구학교실) ;
  • 김미려 (대구한의대학교 한의과대학 약리학교실) ;
  • 양재하 (대구한의대학교 한의과대학 생리학교실) ;
  • 한상원 (대구한의대학교 한의과대학 침구학교실)
  • Received : 2004.07.22
  • Accepted : 2004.07.25
  • Published : 2004.08.20
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Abstract

Objective : In order to prove the effect of Hirudin Herbal-acupuncture this experimental studies were performed by using rats that had neuronal damage due to the Middle Cerebral Artery Occulsion(MCAO). Methods : Microdialysis probes were implanted into the coordinate of striatum of anesthetized rats which consist of sham-operated 8 rats, MCAO-operated 8 rats and Hirudin Herbal-acupuncture administrated 8 rats before MCAO operating. The Hirudin Herbal-acupuncture(0.5mg/kg) was administrated to rats 30 minutes before having an operation causing the MCAO. The surgical excision lead the cross resected brain to the acute ischemic state. The brain was sliced in 2mm thickness and stained with cresyl violet buffer for the measurement of cerebral infarcted area and volume. Results : Based on the result of the tissue inspection for the cerebral ischemic cell, Hirudin Herbal-acupuncture significantly protect neurocytes. Conclusion : We suggest Hirudin Herbal-acupuncture produces protective effects against the neuronal damage induced by MCAO. Therefore, Hirudin Herbal-acupuncture may prevent delayed neuronal death(DND) in selectively vulnerable focal areas of the brain effectively.

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