Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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Inhibitory Effect of Cotesia plutellae Bracovirus (CpBV) on Development of a Non-natural Host, Spodoptera exigua

프루텔고치벌(Cotesia plutellae) 유래 폴리드나바이러스의 비자연 기주 파밤나방(Spodoptera exigua)에 대한 발육 억제 효과

  • Kim Yonggyun (School of Bioresource Sciences, Andong National University) ;
  • Kim Jiwon (School of Bioresource Sciences, Andong National University)
  • 김용균 (안동대학교 생명자원과학부) ;
  • 김지원 (안동대학교 생명자원과학부)
  • Published : 2004.09.01
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Abstract

Polydnavirus is a symbiotic virus of some endoparasitic wasps and plays crucial roles in inhibiting immune responses and retarding development of the parasitized hosts. Cotesia plutellae bracovirus (CpBV) is a polydnavirus suggesting a major causative to change developmental physiology of the parasitized host. Here, we investigated whether CpBV can interrupt development of non-natural host. Beet armyworm, Spodoptera exigua, is used as a non-permissible host for parasitization of C. plutellae. Extract from the calyx region of C. plutellae contained CpBV, which was confirmed by immunoblotting with a polyclonal antibody raised against CpBV. One female equivalent of CpBV extract was injected into hemocoel of late 4th instar larvae of S. exigua. The injected larvae showed delayed larval period, decrease of body weight gain, and inability of pupal metamorphosis. These inhibitory effect of the CpBV extract was rescued by injection along with CpBV antibody, though the antibody itself did not give any effect on development of the larvae. This result clearly shows that CpBV can interrupt developmental physiology of a non-natural host for its symbiotic wasp.

폴리드나바이러스는 일부 내부기생봉의 공생 바이러스로서 기생봉 기생과정중 피기생자의 면역억제와 발육지연에 중요한 역할을 담당한다. 프루텔고치벌(Cotesia plutellae)유래 브라코바이러스(CpBV)는 피기생자의 발육을 교란시키는 주요 원인자로서 작용하는 폴리드나바이러스의 일종이다. 본 연구는 이 CpBV가 비자연기주에게도 발육교란을 유발할 수 있는 지 조사하였다. 이를 위해 프루텔고치별에 의해 기생되지 않는 비기주인 파밤나방(Spodoptera exigua)을 이용하였다. 이용된 CpBV는 프루델고치벌 난소받침(calyx)추출물에서 얻었다. 이 추출물은 CpBV항체에 대해서 뚜렷한 항원-항체반응성으로 CpBV 보유가 입증되었다. 고치벌 암컷 한 마리 CpBV 추출물을 후기 4령의 파밤나방 혈강내로 주입시켰다. CpBV 주입된 파밤나방 유충은 유충기간이 연장되고, 체중증가 가 억제되며, 궁극적으로 변태가 억제되었다. 이러한 CpBV의 발육억제 효과는 이 바이러스의 항체를 함께 주입하여 줌으로 해소되었다. 여기서 항체 단독으로는 파방나방 유충 발육에 아무런 영향을 주지 않았다. 이 결과는 CpBV가 공생 기생봉의 비기주체에 대해서 발육교란 효과를 줄 수 있다는 것을 제시하고 있다.

Keywords

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