Journal of Preventive Medicine and Public Health

The Korean Society for Preventive Medicine (대한예방의학회)
권호 표지

DNA Damage of Lymphocytes in Volunteers after 4 hours Use of Mobile Phone

일부 자원자들의 이동전화 4시간 연속 사용 후 림프구 DNA 손상 평가

  • Ji, Seon-Mi (Department of Preventive Medicine, College of Medicine, Korea University) ;
  • Oh, Eun-Ha (Department of Preventive Medicine, College of Medicine, Korea University, Medical Research Center for Environmental Toxico-Genomics and Proteomics) ;
  • Sul, Dong-Geun (Department of Preventive Medicine, College of Medicine, Korea University, Medical Research Center for Environmental Toxico-Genomics and Proteomics) ;
  • Choi, Jae-Wook (Department of Preventive Medicine, College of Medicine, Korea University) ;
  • Park, Hee-Chan (Department of Preventive Medicine, College of Medicine, Korea University) ;
  • Lee, Eun-Il (Department of Preventive Medicine, College of Medicine, Korea University, Medical Research Center for Environmental Toxico-Genomics and Proteomics)
  • 지선미 (고려대학교 의과대학 예방의학교실) ;
  • 오은하 (고려대학교 의과대학 예방의학교실, 고려대학교 유전체 및 단백체 환경독성 의과학센터) ;
  • 설동근 (고려대학교 의과대학 예방의학교실, 고려대학교 유전체 및 단백체 환경독성 의과학센터) ;
  • 최재욱 (고려대학교 의과대학 예방의학교실) ;
  • 박희찬 (고려대학교 의과대학 예방의학교실) ;
  • 이은일 (고려대학교 의과대학 예방의학교실, 고려대학교 유전체 및 단백체 환경독성 의과학센터)
  • Published : 2004.12.01
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Abstract

Objectives : There has been gradually increasing concern about the adverse health effects of electromagnetic radiation originating from cell phones which are widely used in modern life. Cell phone radiation may affect human health by increasing free radicals of human blood cells. This study has been designed to identify DNA damage of blood cells by electromagnetic radiation caused by cell phone use. Methods : This study investigated the health effect of acute exposure to commercially available cell phones on certain parameters such as an indicator of DNA damage for 14 healthy adult volunteers. Each volunteer during the experiment talked over the cell phone with the keypad facing the right side of the face for 4 hours. The single cell gel electrophoresis assay (Comet assay), which is very sensitive in detecting the presence of DNA strand-breaks and alkali-labile damage in individual cells, was used to assess peripheral blood cells (T-cells, B-cells, granulocytes) from volunteers before and after exposure to cell phone radiation. The parameters of Comet assay measured were Olive Tail Moment and Tail DNA %. Results : The Olive Tail Moment of B-cells and granulocytes and Tail DNA % of B-cells and granulocytes were increased by a statistically significant extent after 4-hour use of a cell phone compared with controls. Conclusion : It is concluded that cell phone radiation caused the DNA damage during the 4 hours of experimental condition. Nonetheless, this study suggested that cell phone use may increase DNA damage by electromagnetic radiation and other contributing factors.

Keywords

References

  1. Wertheimer N, Leeper E, Electrical wiring configurations and childhood cancer. Am J Epidemiol 1979; 109(3): 273-284
  2. A study of Electromagnetic Field Effects on Biological System. Ministry of information and communication Republic of Korea; 2001: (3-4, 47-50, 140-142, 181-202) (Korean)
  3. Annual report on The Statistics, National statistical office Republic of Korea;2003 (Korean)
  4. Dreyer NA, Loughlin JE, Rothman KJ. Causespecific mortality in cellular telephone users. JAMA 1999; 282(19): 1814-1816 https://doi.org/10.1001/jama.282.19.1814
  5. Stang A. Cell phones and radio devices again in the news, Eye melanoma caused by telephoning? MMW Fortschr Med 2001; 143(7): 14
  6. Hardell L, Nasman A, Pahlson A, Hallquist A. Case-control study on radiology work, medical x-ray investigations, and use of cellular telephones as risk factors for brain tunors. Med Gen Med 2002; 2(2): E2
  7. Hardell L, Mild KH, Carlberg M, Case-control study onthe use of cellular and cordless phones and he risk for malignant brain tumours. Int J Radiat Biol 2002; 78(10): 931-936
  8. Richter ED, Beman T, Levy O, Brain cancer with induction periods of less than 10 years in young military radar workers, Arch Environ Health 2002; 57(4): 270-272
  9. Hardell L, Mild KH, Carlberg M, Further aspects on cellular and cordless telephones and brain tumours. lnt J OncoI 2003; 22(2): 399-407
  10. Elwood lM, Epidemiological Studies of Radio Frequency Exposure and Human Cancer. Bioeledromagnetics 2003; 6: S63-S73
  11. de Seze R, Ayoub J, Peray P, Miro L, Touitou Y, Evaluation in humans of the effects of radiocellular telephones on the circadian patterns of melatonin secretion, a chronobiological rhythm marker. J Pineal Res 1999; 27(4): 237-242
  12. Burch JB, Rief JS, Noonan CW, Ichinose T, Bachand AM, Koleber TL, Yost MG. Melatonin metabolite excretion among cellular telephone users, lnt J Radiat Biol 2002; 78 (11): 1029-1036 https://doi.org/10.1080/09553000210166561
  13. Marinelli F, La Sala D, Cicciotti G, Cattini L, Trimarchi C, Putti S, Zamparelli A, Giuliani L, Tomassetti G, Cinti C. Exposure to 900 MHz electromagnetic field induces an unbalance between pro-apcptotic and pro-survival signals in T-lymphcolastoid leukemia CCRF-CEM ells. J Cell Physiol 2004; 198(3): 479-480 https://doi.org/10.1002/jcp.10483
  14. Irmak MK, Fadillioglu E, Gulec M, Erdogan H, Yagmurca M, Akyol O. Elfects of electromagnetic radiation from a cellular telephone on the oxidant and antioxidant levels in rabbits, Cell BiochemFunct 2002; 20(4): 279-283
  15. Moustafa YM, Moustafa RM, Belacy A, Abou-El-Ela SH, Ali FM, Effects of acute exposure to the radio ftequency fields of celluar phones on plasma lipid peroxide and antioxidase activities in human eryfuocytes. J Pharm Biomed Anal 2001; 2(4): 605-608
  16. Malyapa RS, Ahem EW, Straube WL, Moros EG, Pickard WF, Roti Roti JL, Measurement of DNA damage after exposure to electro magnetic radiation in the cellular phone comunication frequency band (835,62 and 847,74 MHz). Radiat Res 1997; 148(6): 618-627
  17. Collins AR, Dusinska M, Gedik CM, Stetina R. Oxidative damage to DNA: do we have a reliable biomarker? Environ Health Perspect 1996; 104 Supp13: 465-469
  18. Giovannelli L, Pitazzi V, Riolo S, Dolara P, Measurement of DNA breaks and oxidative damage in polymorphonuclear and mononuclear white blood cells: a novel approach using the comet assay. Mutat Res 2003; 538(12): 71-80
  19. Singh NP, McCoy MT, Tice RR, Sclneider EL. A simple technique for quantitation of low levels of DNA damage inindividual cells. Exp Cell Res 1988; 175(1): 184-191 https://doi.org/10.1016/0014-4827(88)90265-0
  20. Cho JA, Oh E, SuI D, Lee E, DNA damage in Iymphocqtes after hair dyeing and related factors among women volunteers, Korean J Prev Med 2002; 35(4): 275-281
  21. SuI D, Lee D, Im H, Oh E, Kim J, Lee E. Single strand DNA breaks in T-and B-lymphocytes and granulocytes in workers exposed to benzene, Toxicoi Lett 2002; 134(13): 87-95
  22. Pacini S, Ruggiero M, Sardi I, Aterini S, Gulisano F, Gulisano M. Exposure to global system for mobile communication (GSM) cellular phone radiofrequency alters gene expression, proliferation, and morphology of human skin fibroblasts, Oncol Res 2002; 13(1): 19-24
  23. Carlo GL, Jenrow RS, Scientific progress - wireless phones and brain cancer: currnt state of the science. Med Gen Med 2000; 2(3): E40
  24. Kassie F, Parzefall W, Knasmuller S, Single cell gel electrophoesis assay: a new technique for human biomonitoring studies. Mutat Res 2000; 463(1): 13-31 https://doi.org/10.1016/S1383-5742(00)00041-7
  25. Moller P, loft S, Oxidative DNA damage in human white blood cells in dietary antioxidant intervention studies. Free Radic Biol Med 2002; 76(2): 303-310
  26. Olive PL. The Comet assay. An overview of techniques, Methods Mol Biol 2002; 203: 179-194
  27. Holz O, Jorres R, Kastner A, Magnussen H. Reproducibility of basal and induced DNA single-strand breaks detected by the single-cell gel electrcphoresis assay in human peripheral mononuclear lukocytes, Int Arch Environ Health 1995; 67(5): 305-310
  28. Moller P, Knudsen LE, Loft S, Wallin H. The comet assay as a rapid test in biomonitoring occupational exposure to DNA-damaging agents and effect of confounding factors. Cancer Epidemiol Biomarkers Prev 2000; 9(10): 1005-1015
  29. Sul D, Oh E, Im H, Yang M, Kim CW, Lee E, DNA damage in T- and B-lymphocytes and granulocytes in emission inspection and incineration workers exposed to polycyclic aromatic hydrocarbons. Mutat Res 2003; 538 (1-2): 1C9-119
  30. Wojewodzka M, Kruszewski M, Iwanenko T, Collins AR, Szumiel I. Application of the comet assay for monitoring DNA. damage in workers exposed to chronic low-dose irradiation, I. Strand breakage, Mutat Res 1998; 416(1-2): 21-35
  31. Collins AR, The Comet assay, Principles, applications, and limitations, Methods Mol Biol 2002;203: 163-177
  32. Collins AR, Horvathova E, Oxidative DNA damage, antioxidants and DNA repair: applications of the comet assay, Biochem Soc Trans 2001; 29: 337-341
  33. Olive PL. DNA damage and repair in individual cells: applications of the comet assay in radiology. Int Radiat Biol 1999; 75(4): 395-405 https://doi.org/10.1080/095530099140311
  34. Gedik CM, Boyle SP, Wood SG, Vaughan NJ, Collins AR, Oxidativw stress in humans: validation of biomarkers of DNA damage. Carcinogenesis 2002; 23(9): 1441-1446
  35. Glaab VG, Collins AR, Eisenbrand G, Janzowski C. DNA-damaging potential and glutathione depletion of 2-cyclohexene-1-one in mammalian cells, compared to food relevant 2-alkennals. Mutat Res 2001; 497: 185-197 https://doi.org/10.1016/S1383-5718(01)00260-1
  36. Lankinen MH, Vilpo LM, Vilpo JA, UV- and gamma-irradation-induced DNA single-strand breaks and their repair in human blood granulocytes and lymphocytes. Mutat Res 1996; 352(1-2): 31-38
  37. Vijayalaxmi, Strauss GH, Tice RR. An analysis of gamma-ray-induced DNA damage in human blood leukocytes, lymphocytes and granulocytes, Mutat Res 1993; 292(2): 123-128
  38. Available from: URl: http://mic.news.go.kr/warp/webapp/news/view?section_id=p_sec_l&id=d3d1ca1adaa99738f7a7880c
  39. Johansen C, Boice JJr, McLaughlin J, Olsen J. Cellular telephones and cancer--a nationwide cohort study in Denmark J Natl Cancer Inst 2001;93(3): 203-207
  40. Auvinen A, Hietanen M, Luukkonen R, Koskela RS, Brain tumors and salivary gland cancers among cellular telephone users, Epidemiology 2002; 13(3): 356-359
  41. Wojewodzka M, Kruszewski M, Iwanenko T, Collins AR, Szumiel I. Application of the comet assay for monitoring DNA damage in workers exposed to chronic low-dose irradiation. I. Strand breakage, Mutat Res 1998; 416(1-2): 21-35
  42. Alessio HM, Exercise-induced oxidative stress, Med Sci Sports Exerc 1993; 25(2): 218-224
  43. Moller P, Wallin H, Knudsen LE, Oxidative stress asscciated with exercise, psychological stress and life-style factors. Chem Biol Interact 1996; 102(1): 17-36
  44. Chance WT, Grossman CJ, Newrock R, Bovin G, Yerian S, Schmitt G, Mendenhall C. Effects of electromagnetic fields and gender on neurotransmitters and amino acids in rats, Physiol Behav 1995; 58(4): 743-748 https://doi.org/10.1016/0031-9384(95)00090-6
  45. Smythe JW, Costall B. Mobile phone use facilitates memory in male, but not female, subjects, Neuroreport 2003; 14(2): 243-246 https://doi.org/10.1097/00001756-200302100-00017
  46. Rothman KJ, Chou CK, Morgan R, Balzano Q, Guy AW, Funch DP, Preston-Martin S, Mandel J, Steffens R, Carlo G, Assessment of cellular telephone and other radio frequency exposure for epidemiologic research, Epidemiology 1996; 7(3): 291-298