대한화장품학회지 (Journal of the Society of Cosmetic Scientists of Korea)

  • 제30권3호
  • /
  • Pages.399-404
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  • 2004
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  • 1226-2587(pISSN)
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  • 2288-9507(eISSN)
대한화장품학회 (Society of Cosmetic Scientists of Korea)
권호 표지

Gly-His-Lys 펩타이드가 결합된 키토산과 그의 세포증식 효과에 관한 연구

Gly-His-Lys Conjugated Chitosan and its Cell Proliferation Effects

  • 하병조 (서울보건대학 뷰티아트과) ;
  • 이윤식 (서울대학교 응용화학부) ;
  • 박수남 (서울산업대학교 정밀화학과)
  • Ha Byung-Jo (Department of Beauty Arts, Seoul Health College) ;
  • Lee Yoon-Sik (School of Chemical Engineering, College of Engineering, Seoul National University) ;
  • Park Soo Nam (Department of Fine Chemistry, Seoul National University of Technology)
  • 발행 : 2004.11.01
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초록

새로운 Gly-His-Lys (약자로 GHK)가 결합된 키토산을 $N^{\alpha}-Fmoc$ 아미노산과 BOP 커플링 시약을 사용한 고상법에 의해 제조하였다. 이를 위해 키토산 마이크로 비드를 W/O 에멀젼 상분리법으로 평균 입자크기 70 마이크로미터로 얻었다. GHK 펩타이드는 순차적으로 고상법에 의해 키토산 마이크로비드 위에 커플링 하였다. 아미노산 분석을 실시한 결과 Gly, His, kys의 비율이 1.02:1.13:0.96의 비율로 나타나 이론치와 거의 일치함을 확인할 수 있었다. GHK 펩타이드가 결합된 마이크로비드의 세포증식 효과는 MTT 분석으로 측정하였다. 측정결과 GHK 펩타이드가 결합된 키토산 마이크로비드는 대조군인 펩타이드가 결합되지 않은 키토산 마이크로비드 자체에 비해 높은 세포증식 효과를 보였다.

Novel GHK-conjugated chitosan was prepared by the solid-phase method using $N^{\alpha}-Fmoc$ amino acids/BOP coupling reagent. For this purpose, the chitosan microbeads which had a mean diameter of 70 um were prepared by the W/O emulsion-phase separation method. The GHK was successfully coupled to the chitosan microbeads by stepwise solid-phase method. The result of amino aid analysis was in good agreement with the theoretical values; $Gly_{1.02}\;of\;His_{1.13}\;Lys_{0.96).$. The cell proliferation effect of the GHK-bound chitosan microbeads was measured by MTT assay. We concluded that GHK-bound chitosan microbeads gave higher cell Proliferation effect than chitosan microbeads.

키워드

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