Journal of Periodontal and Implant Science

  • 제34권4호
  • /
  • Pages.759-769
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  • 2004
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  • 2093-2278(pISSN)
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  • 2093-2286(eISSN)
대한치주과학회 (Korean Academy of Periodontology)
권호 표지

키토산이 백서 태자 두개관세포의 세포외기질 발현과 석회화에 미치는 영향

Effect of chitosan on bone matrix expression and mineralization in primary rat calvarial cell

  • 김재철 (전남대학교 치과대학 치주과학교실) ;
  • 최득철 (전남대학교 대학원 치의학과) ;
  • 김영준 (전남대학교 치과대학 치주과학교실, 전남대학교 치의학연구소) ;
  • 정현주 (전남대학교 치과대학 치주과학교실, 전남대학교 치의학연구소) ;
  • 김옥수 (전남대학교 치과대학 치주과학교실, 전남대학교 치의학연구소)
  • Kim, Jae-Cheol (Dept. of Periodontology, Chonnam National University) ;
  • Ciu, De-Zhe (Graduate School, Dept. of Dental Science, Chonnam National University) ;
  • Kim, Young-Joon (Dept. of Periodontology, Chonnam National University, Dental Science Research Institute, Chonnam National University) ;
  • Chung, Hyun-Ju (Dept. of Periodontology, Chonnam National University, Dental Science Research Institute, Chonnam National University) ;
  • Kim, Ok-Su (Dept. of Periodontology, Chonnam National University, Dental Science Research Institute, Chonnam National University)
  • 발행 : 2004.12.31
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초록

Periodontal therapy has dealt primarily with attempts at arresting progression of disease, however, more recent techniques have focused on regenerating the periodontal ligament having the capacity to regenerate the periodontium. The effect of chitosan, a carbohydrate biopolymer extracted from chitin, on periodontal ligament regeneration is of particular interest. The purpose of this study was to evaluate the effect of chitosan on the expression of extracellular matrix proteins in primary rat calvarial cells in Vitro. In the control group, cells was cultured with BGjb media. In the experimental groups, cells were cultured with chitosan in concentration of 0.01, 0.1, 1.0 and 2.0 mg/ml. Then each group was characterized by examining alkaline phosphatase activity at 3 and 7 days, and the ability to produce mineralized nodules of rat calvarial cells at 14 and 21 days. Synthesis of type I collagen (COL-I), osteocalcin (OCN), bone sialoprotein (BSP) was evaluated by RT-PCR at 14 days. The results were as follows: 1. Alkaline phosphatase activity was significantly higher in the concentration of chitosan 0.01mg/ml, 0.1mg/ml and 1.0mg/ml compared to control (p<0.05). 2. The percentage of mineralized bone nodule was more in the concentration of chitosan 0.1mg/ml and 1.0mg/ml than the control. 3. At 14 day culture, the expression of OCN was increased by chitosan in concentration of 1.0 mg/ml and 2.0 mg/ml. These results suggested that chitosan in concentration of 0.1 and 1,0 mg/ml stimulate the extracellular matrix of primary rat calvarial cells and may facilitate the formation of bone.

키워드

참고문헌

  1. Melcher AH: On the reair potential of periodontal tissue. J Periodontal 1976;47:256-260 https://doi.org/10.1902/jop.1976.47.5.256
  2. Iglhaut J, Aukhil I, Simpson DM, Johnston MC, Kock G: Progenitor cell kinetics during tissue regeneration in experimental periodontal wounds. J Periodont Res 1988;23:107-117 https://doi.org/10.1111/j.1600-0765.1988.tb01342.x
  3. McCulloch CAG, Bordin S: Role of fibroblast subpopulations in periodontal physiology and pathology. J Periodont Res 1991;26:144-154 https://doi.org/10.1111/j.1600-0765.1991.tb01638.x
  4. Ripamonti U, Petit JC, Lemmer J, Austin JC: Regeneration of the connective tissue attachment on surgically exposed roots using a fibrinfibronectin adhesive system. J Periodont Res 1987;22:320-326 https://doi.org/10.1111/j.1600-0765.1987.tb01592.x
  5. Mellonig JT, Bowers GM, Bright RW, Lawlence JJ: Clinical evaluation of freeze-dried bone allografts in periodontal osseous defects. J Periodontol 1976;47:125-131 https://doi.org/10.1902/jop.1976.47.3.125
  6. Pitaru S, Tal H, Soldinger M, Noff M: Collagen membranes prevent apical migration of epithelium and support new connective tissue attachment during periodontal wound healing in dogs. J Periodontal Res 1989;24:247-253 https://doi.org/10.1111/j.1600-0765.1989.tb01789.x
  7. Ripamonti U, Reddi AH: Periodontal regeneration; potential role of bone morphogenic proteins. J Periodont Res 1994;29:225-235 https://doi.org/10.1111/j.1600-0765.1994.tb01216.x
  8. Matsuda N, Kumar M, Ramakrishnan PR, Lin WL, Genco RJ, Cho MI: Evidence for up-regulation of epidermal growth factors on rat periodontalligament fibroblastic cells associated with stabilization of phenotype in vitro. Arch Oral Biol 1993;38:559-569 https://doi.org/10.1016/0003-9969(93)90120-B
  9. Casser-Bette M, Murray AB, Closs EI, Erfle V, Schmidt J: Bone formation by osteclast-like cells in a three-dimension cell culture. Calcif Tissue Int 1990;46:46-56 https://doi.org/10.1007/BF02555824
  10. Petet MG: Applications and environmental aspects of chitin and chitosan. J.M.S-Pure Appl Chem 1995;32:629-640
  11. Aspinall GO: Chitin and chitosan in the polysaccharides. New York: Academic press, 1983:386-390
  12. Muzzarelli RA, Baldassarre M, Conti F, Ferrara P, Biagini B: Biological activity of chitosan. Biomaterials 1988;9:247-252 https://doi.org/10.1016/0142-9612(88)90092-0
  13. Sanford PA: Chitosan: Commercial uses and potential applications In Chitin and Chitosan (Skjak-Braek G, Anthonsen T, Sanford P, eds). Elisevier Applied Science, London, 1989:51-70
  14. Prudden JF, Migel P, Hanson P, Friedrich L, Balassa L: The discovery of a potent pure chemical wound-healing accelerator. Am J Surg 1970;119:560-564 https://doi.org/10.1016/0002-9610(70)90175-3
  15. Brandenburg G, Leibroch LG, Shuman R, Malette WG, Qiugley H: Chitosan; A new topical agent for diffuse capillary bleeding in brain tissue. Neurosug 1984;15:9-13 https://doi.org/10.1227/00006123-198407000-00004
  16. Klokkevold PR, Lew DS, Ellis DG, Bertolami CN: Effect of chitosan on lingual hemostasis in rabbits. J Oral Maxillofac Surg 1991;49:858-863 https://doi.org/10.1016/0278-2391(91)90017-G
  17. Sapelli PL, Baldessare V, Muzzarelli RAA, Emanuelli M: Chitosan in dentistry. In: Chitin in Nature and Technology, 1986:507-512
  18. Muzzarelli RA, Biagini G, Pugnaloni A, Baldassarre V: Reconstruction of periodontal tissue with chitosan. Biomaterials 1993;14:39-43 https://doi.org/10.1016/0142-9612(93)90073-B
  19. 계승범, 손성희, 최상묵: Chitosan과 chitosan-cel-lulose를 이용한 차폐막의 골조직 재생유도능력 에 관한 연구. 대한치주과학회지 1998;28:611-630
  20. McCarthy TL, Centrella M, Canalis E: Further biochemical and molecular characterization of primary rat parietal bone cell culture. J Bone Min Res 1988;3: 401-408 https://doi.org/10.1002/jbmr.5650030406
  21. Cho MI, Matsuda N, Lin WL, Moshier A, Ramakrishnan PR: In vitro formation of mineralized nodules by periodontal ligament cells from the rat. Calcif Tissue Int 1992;50:459-467 https://doi.org/10.1007/BF00296778
  22. Melcher AH, McCulloch CAG, Cheong T, Nemeth E, Shiga A: Synthesis of cementum-like tissue in vitro by cells cultured from bone; a light and electron microscope study. J Periodont Res 1986;25:592-612
  23. Marletta G, Iacona F: Chemical and physical property modifications induced by ion irradiation inpolymers. In: Material and Processes for surface and interface engineering. (Y, Pauleau eds) vol 290, NATO-ASI Series, 1995:597-640
  24. Mallette WG, Quigley HJ, Adickes ED: Chitin in nature and technology. New York, Pleum Press, 1986:435-442
  25. Klokkevold PR, Vandemark L, Kenney EB, Bernard GW: Osteogenesis enhanced by chitosan in vitro. J Periodontol 1996;67:1170-1175 https://doi.org/10.1902/jop.1996.67.11.1170
  26. Lahiji A, Sohrabi A, Hungerford D, Frindoza C: Chitosan supports the expression of extracellular matrix proteins in human osteoblasts and chon-drocytes, J Biomed Mater Res 2000;15;51:586-595
  27. 백정원, 이현정, 유윤정, 조규성, 김종관, 최성호: 키토산이 치주인대 섬유아세포에 미치는 영향. 대한치주과학회지 2001;31:823-832
  28. Beertsen W, Theo Van Den B: Calcification of dentinal collagen by cultured rabbit periodontium. The role of alkaline phosphatase. Matrix 1989;9:159-171 https://doi.org/10.1016/S0934-8832(89)80035-6
  29. Anderson HC: Mechanism of mineral formation in bone. Lab Invest 1989;60:320-330
  30. Roach HI: Why does bone matrix contain noncollagenous proteins? The possible roles of osteocalcin, osteonectin, osteopontin and bone sialoprotein in bone mineralization and resorption. Cell Biol Interntl 1994;18:617-628 https://doi.org/10.1006/cbir.1994.1088
  31. Glowacki J, Rey C, Glimcher MJ, Cox KA, Lian J: A role for osteocalcin in osteoblast differentiation. J Cell Biochem 1991;45:292-302 https://doi.org/10.1002/jcb.240450312
  32. Muzzarelli RA, Zucchini C, Ilari P, Pugnaloni A, Belmonte MM, Biagini G, Castaldini C:Osteoconductive properties of methylpytrroli-dine chitosan in an animal model. Biomaterials 1993;14:925-929 https://doi.org/10.1016/0142-9612(93)90134-N