Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

Comparison of Black-pigmented Bacteroides using 16S rRNA analysis between Chronic periodontitis and Aggressive Periodontitis

만성 치주염과 급속 파괴성 치주염에서의 흑색색소 형성 Bacteroides의 분포도 비교

  • Shin, Chul-Woo (Department of Periodontology, College of Dentistry, Yonsei University) ;
  • Choi, Bong-Kyu (Department of Oromaxilofacial Infection and Immunity, Seoul National University) ;
  • Moon, Ik-Sang (Department of Periodontology, College of Dentistry, Yonsei University) ;
  • Kim, Chong-Kwan (Department of Periodontology, College of Dentistry, Yonsei University) ;
  • Cho, Kyoo-Sung (Department of Periodontology, College of Dentistry, Yonsei University)
  • 신철우 (연세대학교 치과대학 치주과학교실) ;
  • 최봉규 (서울대학교 치과대학 구강악안면 감염 및 면역학교실) ;
  • 문익상 (연세대학교 치과대학 치주과학교실) ;
  • 김종관 (연세대학교 치과대학 치주과학교실) ;
  • 조규성 (연세대학교 치과대학 치주과학교실)
  • Published : 2004.06.30
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Abstract

The purpose of the research is to compare the distribution of Black-pigmented Bacteroides between Chronic Periodontitis and Aggressive Periodontitis. P. gingivalis, P. intermedia and P. nigrescens were examined in order to evaluate their distribution in patients with Chronic Periodontitis(CP) and Aggressive Periodontitis(AP). PCR and dot-blots hybridization of 16s rRNA gene were used to compare bacterial distribution of two groups - CP group and AP group, which were divided into two subgroups. Subgingival plaque taken from the diseased sites(pocket $depth{\geq}6$ mm) and healthy sites(pocket $depth{\leq}3$ mm) were grouped into the experimental group and the control group. The result are as follows ; 1. The distribution of P. gingivalis was 98.33% for chronic Periodotitis(CP), 94.17% for Aggressive Periodontitis(AP), the distribution of P. intermedia was 77.50% for CP, 64.17% for AP, and the distribution of P. nigrescens was 35.00%, 29.17%. In all 3 types of bacteria, CP group showed higher distribution compared to AP group, but only P. intermedia showed statistically significant difference. 2. In the case of CP, every type of bacteria showed higher distribution in the experimental group with statistically significant difference. 3. In the case of AP, every type of bacteria also showed higher distribution in the experimental group, but P. gingivalis and p..intermedia showed the result with statistically significant difference, and the other did not 4. In 3 all bacteria type, N-AP showed higher distribution than N-CP without statistically significant difference These results suggest that the comparison of the distribution of Bacteroides between Chronic Periodontitis and Aggressive Periodontitis has no statistically significant difference, except P. intermedia.

Keywords

References

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