Asian Journal of Turfgrass Science (아시안잔디학회지)

Turfgrass Society of Korea (한국잔디학회)
권호 표지

Herbicide Resistant Turfgrass(Zoysia japonica cv. 'Zenith') Plants by Particle bombardment-mediated Transformation

  • Lim Sun-Hyung (Turfgrass & Environmental Research Institute, Samsung Everland) ;
  • Kang Byung-Chorl (Turfgrass & Environmental Research Institute, Samsung Everland) ;
  • Shin Hong-Kyun (Turfgrass & Environmental Research Institute, Samsung Everland)
  • Published : 2004.12.01
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Abstract

Transgenic zoysiagrass (Zoysia japonica cv. Zenith) plants have been obtained by particle bombardment of embryogenic callus with the plasmid pSMABuba, which contains hygromycin resistance (hpt) and bialaphos resistance (bar) genes. Parameters on DNA delivery efficiency of the particle bombardment were partially optimized using transient expression assay of a chimeric $\beta-glucuronidase$(gusA) gene driven by the CaMV 35S promoter. Stably transfarmed zoysiagrass plants were recovered with a selection scheme using hygromycin. Transgenic zoysiagrass plants were confirmed by PCR analysis with specific primer for bar gene. Expression of the transgene in transformed zoysiagrass plants was demonstrated by Reverse transcriptase (RT)-PCR analysis. All the tested transgenic plants showed herbicide BastaR resistance at the field application rate of $0.1\%-0.3\%$.

Keywords

References

  1. Asano Y, Ito Y, Fukami M, Sugiura K, and A. Fujiie. 1998. Herbicide-resistant transgenic creeping bentgrass plants obtained by electroporation using an altered buffer. Plant Cell Reports 17:963-967 https://doi.org/10.1007/s002990050518
  2. Asano Y and M. Ugaki. 1994. Transgenic plants of Agrostis alba obtained by electroporation-mediated direct gene transfer into protoplasts. Plant Cell Reports 13:243-246
  3. Cao J, Duan X, McElroy D, and R. Wu. 1992. Regeneration of herbicide resistant transgenic rice plants following microprojectile-mediated transformation of suspension culture cells. Plant Cell Reports. 11:586-591
  4. Chai ML and DH Kim. 2000. Agrobacterium-mediated transformation of Korean lawngrass (Zoysia japonica). J. Kor. Soc. Hort. Sci. 41:455-458
  5. Chai ML, Senthil K, Mo SY, Chung YS, Cho SH, Shin JS, Park MH, and DH Kim. 2000. Embryogenic callus induction and Agrobacterium-mediated transformation in bentgrass (Agrostis spp.). J. Kor. Soc. Hort. Sci. 41:450-454
  6. Dalton SJ, Bettany AJE, Timms E, and P. Morris. 1999. Co-transformation, diploid Lolium perenne (perennial ryegrass), Lolium multiflorum (Italian ryegrass) and Lolium temulentum (darnel) plants produced by microprojectile bombardment. Plant Cell Reports 18:721-726 https://doi.org/10.1007/s002990050649
  7. Datta SK, Datta K, Soltanifar N, Donn G, and I. Potrykus. 1992. Herbicide-resistance indica rice plants from IRRI breeding line 72 after PEG-mediated transformation of protoplasts. Plant Mol BioI 20:619-629
  8. De Block M, Botterman J, Vandwiele M, Dockx J, Thoen C, Gossele V, Rao Movva N, Thmpson C, van Montagu M, and J. Leemans. 1987. Engineering herbicide resistance in plants by expression of detoxifying enzyme. EMBO J. 9:2513-2518
  9. De Greef W, Delon R, De Block M, Leemans J, and J. Botterman. 1989. Transformation of Brassica napus and Brassica oleracea using Agrobacterium tumefaciens and expression of the bar and neo genes in the transgenic plants. Plant Physiol 91:694-701 https://doi.org/10.1104/pp.91.2.694
  10. Fromm ME, Morrish F, Armstrong C, Williams R, Thomas J, and TM Klein. 1990. Inheritance and expression of chimeric genes in the progeny of transgenic maize plants. Bio/I'echnology 8:833-839
  11. Gordon-kamm WJ, Spencer TM, Mangano ML, Adams TR, Danies RJ, Start WG, O'Brien JV, Chamber SA, Adams WR, Willetts NG, Rice TB, Mackay CJ, Krueger RW, Kausch AP, and PG Lemaux. 1990. Transformation of maize cells and regeneration of fertile transgenic plant. The Plant Cell 2:603-618 https://doi.org/10.2307/3869124
  12. Ha S-B, Wu F-S, and TK Throne. 1992. Transgenic turf-type tall fescue (Festuca arundinacea Screb.) plants regenerated from protoplasts. Plant Cell Reports 11:601-604
  13. Hartman CL, Lee L, Day PR, and NE Tumber, 1994. Herbicide resistant turfgrass (Agrostis palustris Huds.) by biolistic transformation. Bio/fechnology 12:919-923 https://doi.org/10.1038/nbt0994-919
  14. Inokuma C, Sugiura K, Imaizumi N, and C Cho. 1998. Transgenic japanese lawngrass (Zoysia japonica Steud.) plants regenerated from protoplasts. Plant Cell Reports 17:334-338 https://doi.org/10.1007/s002990050403
  15. J$ddot{a}$hne A, Becker D, Brettschneider R, and H. L?rz. 1994. Regeneration of transgenic, microspore-derived, fertile barley. Theor Appl Genet. 89:525-533
  16. Lee Y-H, Lee S-B, Suh S-C, Byun M-O, and HI Kim. 2000. Herbicide resistant cabbage (Brassica oleracea ssp. capitata) plants by grobacterium-mediated transformation. J of Plant Biotechnology 2:35-41
  17. Park GH, and BJ Ahn. 1998. Electroporation conditions for DNA transfer into somatic embryogenic cells of Zoysia japonica. Korean J. Plant Tissue Culture 25:13-19
  18. Spangenberg G, Wang ZY, Wu XL, Nagel J, and I. Potrykus. 1995. Transgenic perennial ryegrass (Lolium perenne) plants from mieroprojectile bombardment of embryogenic suspension cells. Plant Sci 108:209-217 https://doi.org/10.1016/0168-9452(95)04135-H
  19. Spencer TM, O'Brien JV, Start WG, Adams TR, Gordon-Kamm WJ, and PG Lemaux. 1992. Segregation of transgenes in maize. Plant Mol Biol 18:201-210 https://doi.org/10.1007/BF00034949
  20. Thompson CJ, Movva NR, Tizard R, Crameri R, Davies JE, Lauwereys M, and J. Botterman. 1987. Characterization of the herbicide-resistance gene bar from Streptomyces hygroscopicus. The EMBO Journal 6:2519-2523
  21. Uchimiya H, Iwata M, Nojiri C, Samarajeewa PK, Takamatsu S, Ooba S, Anzai H, Christensen AH, Quail PH, and S. Toki. 1993. Bialaphos treatment of transgenic rice plants expressing a bar gene prevents infection by the sheath blight pathogen (Rhizoctonia solani). Biotechnology 11:835-836 https://doi.org/10.1038/nbt0793-835
  22. Vasil V, Srivastava V, Castillo AM, Fromm ME, and IK Vasil. 1992. Herbicide resistant fertile transgenic wheat plants obtained by microprojectile bombardment of regenerable embryogenic callus. Bio/Technology 10:667-674. https://doi.org/10.1038/nbt0692-667
  23. Wang Z, Takamizo T, Iglesias VA, Osusky M, Nagel J, Potrykus I, and G. Spangenberg. 1992. Transgenic plants of tall fescue (Festua arundinacea Screb.) obtained by direct gene transfer to protoplasts. Bio/Technology 10:691-696 https://doi.org/10.1038/nbt0692-691
  24. Weeks JT, Aderson OD, and AE Blechl. 1993. Rapid production of multiple independent lines of fertile transgenic wheat (Triticum aestivum). Plant Physiol. 102:1077-1084
  25. Ye X, Wang Z-Y, Wu X, Potrykus I, and G. Spangenberg. 1997. Transgenic italian ryegrass (Lolium multiflorum) plants from microprojectile bombardment of embryogenic suspension cells. Plant Cell Reports 16:379-384
  26. Zhong H, Bolyard MG, Srinivasan C, and MB Sticklen. 1993. Transgenic plants of turfgrass (Agrostis palustris Huds.) from microprojectile bombardment of embryogenic callus. Plant Cell Reports 13:1-6