The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
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Development of Molecular Detection of Three Species of Seed-Transmissible Viruses Useful for Plant Quarantine

  • Lee, Bo-Young (Plant Virus GenBank, Division of Environmental and Life Sciences, Seoul Women's University) ;
  • Lim, Hee-Rae (Plant Virus GenBank, Division of Environmental and Life Sciences, Seoul Women's University) ;
  • Choi, Ji-Yong (Plant Virus GenBank, Division of Environmental and Life Sciences, Seoul Women's University) ;
  • Ryu, Ki-Hyun (Plant Virus GenBank, Division of Environmental and Life Sciences, Seoul Women's University)
  • 발행 : 2004.12.31
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초록

Three pairs of specific primers were developed for rapid and precise RT-PCR detection of three seed-transmissible viruses, namely Peanut clump virus (PCV, Pecluvirus), White clover mosaic virus (WCIMV, Potexvirus) and Carrot red leaf virus (CaRLV, Luteovirus). Each primer set was found in conserved region through multiple sequence alignment in the DNAMAN. Total nucleic acids extracted from PCV-, WCMV-, and CaRLV-infected seeds and healthy plants were used for RT-PCR detection using each virus-specific primer, Sizes of PCV, WCIMV, and CaRLV PCR products were 617bp (PCV-uni5 and PCV-uni3 primers), 561bp (WCMV-CP5 and WCMV-CP3 primers), and 626bp (CL1-UP and CL2-DN primers); which corresponded to the target sizes. Nucleotides sequences of each amplified cDNA were confirmed which belonged to the original virus. This study suggests that these virus-specific primer sets can specifically amplify viral sequences in infected seeds. Thus, they can be used for specific detection of three viruses (PCV, WCMV and CaRLV) from imported seed samples for plant quarantine service.

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참고문헌

  1. Forster, R. L., Bevan, M. W., Harbison, S. A and Gardner, R. C. 1988. The complete nucleotide sequence of the potexvirus white clover mosaic virus. Nucleic Acids Res. 16:291-303 https://doi.org/10.1093/nar/16.1.291
  2. Ghewande, M. P. and Nandagopal, V. 1997. Integrated pest management in groundnut (Arachis hypogaea L.) in India. Integ. Pest Mana. Rev. 2:1-15 https://doi.org/10.1023/A:1018488326980
  3. Hobbs, H. A, Reddy, D. V. R. and Reddy, AS. 1987. Detection of a mycoplasma-like organism in peanut plants with witches' broom using indirect enzyme-linked immunosorbent assay (ELISA). Plant Pathol. 36:164-167 https://doi.org/10.1111/j.1365-3059.1987.tb02216.x
  4. Hull, R. 2002. Matthew$\'{s}$Plant Virology, 4th ed.. Academic Press, London, pp.546-548
  5. Maule, A. J. and Wang D. 1996. Seed transmission of plant viruses: a lesson in biological complexity. Trends Microbiol. 4:153-158 https://doi.org/10.1016/0966-842X(96)10016-0
  6. Mink, G I. 1993. Pollen and seed-transmitted viruses and viroids. Annu. Rev. Phytopathol. 31 :375-402 https://doi.org/10.1146/annurev.py.31.090193.002111
  7. Murant, A F., Robinson, D. J. and Gibbs, M. J. 1995. Genus Umbravirus. In: Murphy, F. A, Fauquet, C. M., Bishop, D. H. L., et al. (eds), Virus taxonomy, the classification and nomenclature of viruses. Springer, New York
  8. Reddy, A. S., Hobbs, H. A., Delfosse, P., Murthy A K. and Reddy, D. V. R. 1998. Seed transmission of Indian peanut clump virus (IPCV) in peanut and millets. Plant Dis. 82:343-346 https://doi.org/10.1094/PDIS.1998.82.3.343
  9. Reddy, D. V. R., Delfosse, P. and Mayo, M. A. 1999. Pecluviruses. In: Webster, R., Granoff, A. (eds) Encyclopedia ofvirology. Academic Press, San Diego, pp.1196-1200
  10. Reddy, D. V. R., Noh, B. L., Hobbs, H. A and Reddy, A S. 1988. Clump virus in India: isolates, host range, transmission and management. In: Cooper JI, Asher MJC (eds) Developments in applied biology 2, viruses with fungal vector. AAB, Wellesbourne, Warwick, UK. pp.239-246
  11. Roberts, I. M., Wang, D., Thomas, C. L. and Maule, A. J. 2003. Pea seed-borne mosaic virus seed transmission exploits novel symplastic pathways to infect the pea embryo and is, in part, dependent upon chance. Protoplasma 222:31-43 https://doi.org/10.1007/s00709-003-0015-5
  12. Ryu, K. H., Choi, C. W., Choi, J. K. and Park, W. M. 1995. Cloning ofthe 3'-terminal region encoding movement and coat proteins of a Korean isolate of odontoglossum ringspot virus. Arch. Virol. 140:481-490 https://doi.org/10.1007/BF01718425
  13. Stubbs, L. L. 1948. A new disease of carrots; its transmission host range and control. Aust. J.Sci. Res. B1:303-332
  14. Thouvenel, J. C., Germani, G and Pfeiffer, P. 1976. Some properties of peanut clump, newly discovered virus. Ann. Appl. Biol. 84:311-320 https://doi.org/10.1111/j.1744-7348.1976.tb01774.x
  15. Thouvenel, J. C., Fauquet, C. and Dollet, M. 1988. Peanut clump virus in West Africa. In; Cooper, J. I. and Asher, M. J. C. (eds) Developments in applied biology 2, viruses with fungal vectors. AAB, Wellesboume, Warwick, UK, pp.247-254
  16. Wang, D. and Maule, A. J. 1994. A model for seed transmission ofa plant virus: Genetic and structural analyses ofpea embryo invasion by pea seed-borne mosaic virus. Plant Cell 6:777-787 https://doi.org/10.1105/tpc.6.6.777
  17. Watson, M. A, Serfeant, E. P. and Lennon, E. A 1964. Carrot mottle dwarf and parsnip mottle virus. Ann. Appl. BioI. 54:153 https://doi.org/10.1111/j.1744-7348.1964.tb01179.x

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