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A New Method for Sclerotial Isolation of Two Species of Sclerotium from Infested Soils

  • Kwon, Mi-Kyung (Plant Pathology Division, National Institute of Agricultural Science of Technology, Rural Development Administration) ;
  • Shim, Hong-Sik (Plant Pathology Division, National Institute of Agricultural Science of Technology, Rural Development Administration) ;
  • Yeh, Wan-Hae (Plant Pathology Division, National Institute of Agricultural Science of Technology, Rural Development Administration) ;
  • Kim, Taek-Soo (Plant Pathology Division, National Institute of Agricultural Science of Technology, Rural Development Administration) ;
  • Cho, Weon-Dae (Plant Pathology Division, National Institute of Agricultural Science of Technology, Rural Development Administration) ;
  • Kim, Choong-Hoe (Plant Pathology Division, National Institute of Agricultural Science of Technology, Rural Development Administration) ;
  • Kim, Yong-Ki (Plant Pathology Division, National Institute of Agricultural Science of Technology, Rural Development Administration)
  • 발행 : 2004.12.31

초록

White rot on Allium crops recently had a high incidence with incrensed cultivating areas of tropical garlic types in Korea. Two types of Sclerotium have known as causal agents that produce different size and shapes of sclerotia in infested fields. Therefore, we developed a new method for isolation of sclerotia from infested field soils that can be used for ecological study of Sclerotium spp. and establishment of control strategy. Soil samples collected from heavily infested fields were evenly mixed and placed on a automatic sieve shaker connected with tap water, After 10 min of shaking, residues on 0.5 mm and 0.25 mm sieves were separately collected and suspended with 70% sugar solution, which method floats sclerotia in aqueous layer, Then, floated fraction was carefully separated and mixed with a same volume of 1% sodium hypochlorite solution to differentiate with organic materials. This method provides a direct count of sclerotia under a dissecting microscopy.

키워드

참고문헌

  1. Adams, P. B. 1979. A rapid method for quantitative isolation of sclerotia of Sclerotinia minor and Sclerotium cepivorum from soil. Plant Dis. Rep. 63:349-351
  2. Cho, W. D., Kim, W. G, Hong, S. K. and Kim, W. S. 2002. Sclerotium spp. associated with occurrence ofa new disease, globular Sclerotium rot ofgarlic. Plant Path J. 18:372 (Abstract)
  3. Coley-Smith, J. R. 1959. Studies of the biology of Sclerotium cepivorum Berk. III. Host range: persistence and viability of sclerotia. Ann. Appl. Bioi. 47:511-518 https://doi.org/10.1111/j.1744-7348.1959.tb07283.x
  4. Coley-Smith, J. R. and Javed, Z. U. R. 1970. Testing the viability offungal sclerotia. Ann. Appl. Bioi. 65:289-301
  5. Gerbrandy, S. J. 1992. Effect of different temperature treatments on dormancy of sclerotia of ten isolates of Sclerotium cepivorum. Neth. J. Plant Pathol. 98:269-276 https://doi.org/10.1007/BF01974385
  6. Harper, G E. and Stewart, A. 2000. Magnetic separation technique for the isolation of sclerotia of Sclerotium cepivorum from iron-rich soil particles. Soil Biol. Biotechnol. 32:135-137 https://doi.org/10.1016/S0038-0717(99)00144-3
  7. McCain, A. H. 1967. Quantitative recovery of sclerotia ofSclerotium sepivorum from field soil. Phytopathology 57: 1007 (Abstract)
  8. Papavizas, G C. 1972. Isolation and enumeration ofpropagules of Sclerotium cepivorum from soil. Phytopathology 62:545-549 https://doi.org/10.1094/Phyto-62-545
  9. Scott, M. R. 1956. Studies of biology of Sclerotium cepivorum Berk. I. Growth of the mycelium in soil. Ann. Appl. Bioi. 44:576-586 https://doi.org/10.1111/j.1744-7348.1956.tb02155.x
  10. Summer, D. R. 1995. White ort. In: Compendium of onion and garlic diseases. Schwartz, H. F. and Mohan, S. K. eds. The APS Press, St. Paul, Minnesota, USA
  11. Utkhede, R. S. and Rahe, J. E. 1979. Wet-sieving floatation technique for isolation of sclerotia of Sclerotium cepivorum from muck soil. Phytopathology 69:295-297 https://doi.org/10.1094/Phyto-69-295

피인용 문헌

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  2. Suppression of Citrus Melanose on the Leaves Treated with Rhizobacterial Strains after Inoculation with Diaporthe citri vol.18, pp.4, 2012, https://doi.org/10.5423/RPD.2012.18.4.331