Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Spatholobus suberectus Water Extract induces Apoptotic Cell Death via Inhibition of Cell Cycle in Jurkat Human Leukemia Cell Line

계혈등 추출물이 Jurkat T 임파구의 세포고사 및 세포주기 억제에 미치는 효과

  • Cho Nam Su (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Jung Woo Cheol (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Na Heon Sik (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Song Young Jun (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lee Kye Seung (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lee In (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Jeon Byung Hun (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Moon Byung Soon (Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 조남수 (원광대학교 한의과대학 내과학교실) ;
  • 정우철 (원광대학교 한의과대학 내과학교실) ;
  • 나헌식 (원광대학교 한의과대학 내과학교실) ;
  • 송영준 (원광대학교 한의학전문대학원) ;
  • 이계승 (원광대학교 한의학전문대학원) ;
  • 이인 (원광대학교 한의과대학 내과학교실) ;
  • 전병훈 (원광대학교 한의과대학 병리학교실) ;
  • 문병순 (원광대학교 한의학전문대학원)
  • Published : 2004.02.01
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Abstract

Spatholobus suberectus belonging the family Leguminosae has been used for promoting blood circulation, removing blood stasis, tonifying the blood, relaxing tendons, stopping internal bleeding and eliminating dampness in oriental traditional medicine. This study investigates whether the water extracts of S. suberectus induce apoptotic cell death in Jurkat T-acute lymphoblastic leukemia (ALL) cells. Jurkat cells were increased inhibitions of cell viability in a concentration-dependent manner by S. suberectus, as measured by cell morphology. The capability of S. suberectus to induce apoptosis was associated with proteolytic cleavage of specific target protein such as poly (ADP­ribose)polymerase protein suggesting the possible involvement of caspases. The purpose of the present study is also to investigate the effect of S. suberectus on cell cycle progression. G1 checkpoin related gene products tested (cyclin D1, cyclin dependent kinase 4, retinoblastoma, E2Fl) were decreased in their protein levels in a dose-dependent manners after treatment of the extract. These results indicate that the increase of apoptotic cell death by S. suberectus may be due to the inhibition of cell cycle progression in wild type p53-lacking Jurkat cells.

Keywords

References

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