Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Inhibitory Effect of Phellinus Igniarius water extract on TNF-$\alpha$, IL-1$\beta$, IL-6 and Nitric Oxide Production in lipopolysaccharide - activated Raw 264.7 cells

상황 물추출물이 LPS로 유도된 Raw 264.7 cell에서의 TNF-$\alpha$, IL-1$\beta$, IL-6 및 Nitric Oxide production에 미치는 영향

  • Published : 2004.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

Phellinus igniarius has been clinically used for the treatment of hemorrhoidal fistula, dysmenorrhea and the prevention of cancer in traditional oriental medicine. Recent studies showed that Phellinus igniarius produced anti-cancer, anti-metastasis and immuno-modulatory effects, There is lack of studies regarding the effects of Phellinus igniarius on the immunological activities. The present study was conducted to evaluate the effect of Phellinus igniarius on the regulatory mechanism of cytokines and nitric oxide (NO) for the immunological activities in Raw 264,7 cells. After the treatment of Phellinus igniarius water extract, cell viability was measured by MTT assay, NO production was monitored by measuring the nitrite content in culture medium. COX-2 and iNOS were determined by Immunoblot analysis, and levels of cytokine were analyzed by sandwich immunoassays. Results provided evidence that Phellinus igniarius inhibited the production of nitrite and nitrate (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β) and interleukin-6 (IL-6), and the activation of phospholylation of inhibitor κBα (p-IκBα) in Raw 264.7 cells activated with lipopolysaccharide (LPS). These findings suggest that Phellinus igniarius can produce anti-inflammatory effect, which may play a role in adjunctive therapy in Gram-negative bacterial infections.

Keywords

References

  1. 중약대사전 김창민;신민교;안덕균;이경순
  2. 동의보감 허준
  3. 동의생리병리학회지 v.17 no.4 산지별 상황버섯의 사이토카인 조절효과에 관한 연구 김진수;김정효;이효정;길재호;김성훈;김동희
  4. 동의생리병리학회지 v.16 no.2 캄보디안 상황버섯의 항암 및 면역조절작용에 대한 연구 이효정;이효정;박정민;송규용;강경선;김성훈
  5. Immunopharmacology v.41 no.2 The inhibitory effect of polysaccharides isolated from Phellinus linteus on tumor growth and metastasis. Han SB;Lee CW;Jeon YJ;Hong ND;Yoo ID;Yang KH;Kim HM.
  6. J Ethnopharmacol. v.84 no.2-3 Cytotoxic and antitumor activities of a polypore macrofungus, Phellinus rimosus (Berk) Pilat. Ajith TA;Janardhanan KK
  7. Food chemistry. v.82 antioxidants and free radical scavenging activity of Phellinus baumii (phellinus of Hymenochaetaceae) extracts. Shon MY;Kim TH;Sung NJ
  8. Biotechnol Lett. v.25 no.2 Inhibition of cytochrome P450 isozymes in rat liver microsomes by polysaccharides derived from Phellinus linteus. Shon YH;Nam KS.
  9. The Koren journal of mycology. v.26 no.1 Immuno-stimulating activity of Phellinus linteus. Song CH;Ra KS;Yang BK;Jeon YJ.
  10. J Microbiol Biotechnol. v.10 IL-6 mRNA Expression in Mouse Peritoneal Macrophages and NIH3T3 Fibroblasts in Response to Candida albicans. Lee YS;Kim HS;Kim SK;Kim SD.
  11. J Immunol. v.144 Cytolytic mechanism of activated macrophages. Tumor necrosis factor and L-arginine-dependent mechanism acts as synergistically as the major cytolytic mechanism of activated macrophages. Higuchi M;Higashi N;Taki H;Osawa T
  12. Proc. Soc. Exp. Biol. Med. v.211 Cytokines and nitric oxides in islet inflammation and diabetes. McDaniel ML;Kwon G;Hill JR;Marshall CA;Corbett JA
  13. J. Exp. Med. v.181 Intraislet release of interleukin-1 inhibits beta cell function by inducing beta cell expression of inducible nitric oxide syntheses. Corbett JA;Mac Daniel ML
  14. Cytokine v.6 TNF and IFN$\gamma$ potentiate the deleterious effects of IL-1$\beta$ on mouse pancreatic islets mainly via generation of nitric oxide. Cetkovic-Cvrlje M;Eizirik DL.
  15. 대한화학회지 v.39 산화질소(Nitric Oxide) 이로운가? 해로운가?. 산화질소의 화학과 응용. 황광진
  16. Am J Chin Med v.28 Enhanced expression of inducible nitric oxide synthase by Juzen-taiho-to in LPS-activated RAW264.7 cells, a murine macrophage cell line. Kawamata H;Ochiai H;Mantani N;Terasawa K.
  17. Eur J Pharmacol v.406 Suppression of inducible nitric oxide synthase expression in RAW 264.7 macrophages by two-carboline alkaloids extracted from Melia azedarach. Lee BG;Kim SH;Zee OP;Lee KR;Lee HY;Han JW;Lee HW.
  18. J Ethnopharmacol v.76 Inhibitory effects of methanol extract of Cyperus rotundus rhizomes on nitric oxide and superoxide production by murine macrophage cell line, RAW 264.7 cells. Seo WG;Pae HO;Oh GS;KY Chai;Kwon TO;YG Yun;NY Kim;HT Chung.
  19. 동의생리병리학회지 v.17 no.3 향부자 열수추출물의 Nitric oxide 생성 및 iNOS 유전자 발현에 미치는 영향 이영선;한옥경;신상우;박종현;권영규
  20. Gen Pharmacol v.35 Inhibitory effect of ethyl acetate fraction from Cudrania tricuspidata on the expression of nitric oxide synthase gene in RAW 264.7 macrophages stimulated with interferon-and lipopolysaccharide. Seo WG;Pae HO;Oh GS;Chai KY;Yun YG;Kwon TO;Chung HT.
  21. 대한한의학방제학회지 v.10 no.2 활성화된 설치류 RAW 264.7 대식세포에서 당귀에탄올 추출물의 항염증 효과 장선일;김형진;황기명;배현옥;윤용갑;정헌택;김윤철
  22. Food Chem Toxicol. v.40 no.8 Anti-tumor promoting potential of selected spice ingredients with antioxidative and anti-inflammatory activities: a short review. Surh YJ.
  23. Mutat Res. Molecular mechanisms underlying chemopreventive activities of anti-inflammatory phytochemicals: down- regulation of COX-2 and iNOS through suppression of NF-kappa B activation. Surh YJ;Chun KS;Cha HH;Han SS;Keum YS;Park KK;Lee SS.
  24. British journal of pharmacology. v.139 Inhibition of lipopolysaccharide-inducible nitric oxide synthase, TNF-$\alpha$ and COX-2 expression by sauchinone effects on I-$\kappa$B$\alpha$ phosphorylation, C/EBP and AP-1 activation. Lee AK;Sung SH;Kim YC;Kim SG
  25. Am J Pathol. v.159 no.2 New insights into the role of nuclear factor-kappaB in cell growth regulation. Chen F;Castranova V;Shi X
  26. Neuropeptides v.37 no.6 Production of tumor necrosis factor-alpha, interleukin 1-beta, interleukin 2, and interleukin 6 by rat leukocyte subpopulations after exposure to substance P. Delgado AV;McManus AT;Chambers JP
  27. Nature v.333 Vascular endothelial cells synthesize nitric oxide from L-arginine. Palmer RM;Ashton DS;Moncada S
  28. Gut v.47 Inducible nitric oxide synthase; a little bit og good in all of us. Kubes p.
  29. Life Sci v.69 Camptothecin suppresses nitric oxide biosynthesis in RAW 264.7 macrophages. Chiou WF;Chou CJ;Chen CF
  30. J Ethnopharmacol v.76 The aqueous extract of Rhodiola sachalinensis root enhances the expression of inducible nitric oxide synthase gene in RAW264.7 macrophages. Seo WG;Pae HO;Oh GS;Kim NY;Kwon TO;Shin MK;Chai KY;Chung HT
  31. Curr Opin Pharmacol. v.4 no.2 Cyclooxygenase-2 and inflammation in atherosclerosis. Linton MF;Fazio S
  32. 동의생리병리학회지 v.16 no.5 오미소독음이 마우스 복강 대식세포에서 NO의 분비과정에 미치는 영향 박혜준;윤화정;윤정원;윤소원;고우신
  33. Ann Intern Med v.119 Plasma cytokine and endotoxin levels correlate with survival in patients with the sepsis syndrome. Casey LC;Balk RA;Bone RC
  34. Eur J Clin Invest. v.23 Pancreatic beta-cell function and interleukin-1b in plasma during the acute phase response in patients with major burn injuries. Wogensen L;Jensen M;Svensson P;Worsaae H;Welinder B;Nerup J
  35. Transplantation v.63 Interleukin 1 receptor blockade reduces tumor necrosis factor production, tissue injury, and mortality after hepatic ischemia-reperfusion in the rat. Shito M;Wakabayashi G;Ueda M;Shimazu M;Shirasugi N;Endo M;(et al.)
  36. Surgery. v.135 no.5 Interleukin-1 upregulates anaphylatoxin receptors on mononuclear cells. Takabayashi T;Shimizu S;Clark BD;Beinborn M;Burke JF;Gelfand JA