Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effects of Dangguijakyak-san Extract on Nitric Oxide Production and Cytokine Gene Expression in RAW 264.7 Macrophages Cell

당귀작약산이 마우스 대식세포주의 NO 생성 및 사이토카인 유전자 발현에 미치는 영향

  • Shin Sang Woo (Department of Pathology, College of Oriental Medicine, Daegu Haany University) ;
  • Lee Young Sun (Department of Pathology, College of Oriental Medicine, Daegu Haany University) ;
  • Park Jong Hyun (Department of Pathology, College of Oriental Medicine, Daegu Haany University) ;
  • Kwon Taeg Kyu (School of Medicine, Keimyung University) ;
  • Suh Seong Il (School of Medicine, Keimyung University) ;
  • Kwon Young Kyu (Department of Physiology, College of Oriental Medicine, Daegu Haany University)
  • 신상우 (대구한의대학교 한의과대학 병리학교실) ;
  • 이영선 (대구한의대학교 한의과대학 병리학교실) ;
  • 박종현 (대구한의대학교 한의과대학 병리학교실) ;
  • 권택규 (계명대학교 의과대학) ;
  • 서성일 (계명대학교 의과대학) ;
  • 권영규 (대구한의대학교 한의과대학 생리학교실)
  • Published : 2004.10.01
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Abstract

The present study was conducted to evaluate the immunomodulatory effects of Dangguijakyak-san(당귀작약산). We investigated the effects of cell proliferation in mouse spleen cell and RAW 264.7 macrophages cells. Dangguijakyak-san enhanced mitogenic activity in the dose-response manner in mouse spleen cells and RAW 264.7 macrophages cells. In nitric oxide (NO) synthesis and iNOS mRNA expression by Dangguijakyak-san, Dangguijakyaksan alone had an effect on NO synthesis and iNOS mRNA expression in RAW 264.7 cells. NO production and iNOS mRNA expression which is excessively induced by LPS decreased after treatment of Dangguijakyak-san. The expressions of cytokine gene by Dangguijakyak-san investigated using reverse transcription polymerase chain reaction (RT-PCR). In RT-PCR, IL-1α, IL-1β and IL-6 mRNA expressions induced in Dangguijakyak-san-treated RAW 264.7 cells. These data indicate that 1) Dangguijakyak-san can modulate various immune response and 2) the immunomodulatory effects of Dangguijakyak-san may be, in part, associated with the regulation of NO synthesis, the expressions of these cytokine as well as the mitogenic effect on spleen cells and macrophages cells.

Keywords

References

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