Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Immunoregulatory Action of OGAPI

오가피의 면역조절작용

  • Kim Nam Seok (Department of Medicinal Biotechnology, Woosuk University) ;
  • Kwon Jin (Department of Medical Administration, Kunjang College) ;
  • Koh Ha Young (Department of Food Nutrition, Woosuk University) ;
  • Choi Dong Seong (Department of Medicinal Biotechnology, Woosuk University) ;
  • Oh Chan Ho (Department of Medicinal Biotechnology, Woosuk University)
  • 김남석 (우석대학교 의약생명공학과) ;
  • 권진 (군장대학 의무행정과) ;
  • 고하영 (우석대학교 식품영양학과) ;
  • 최동성 (우석대학교 의약생명공학과) ;
  • 오찬호 (우석대학교 의약생명공학과)
  • Published : 2004.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose of this research was to investigate the immunoregulatory effect and the leukemia cell apoptosis of EtOH extract of OGAPI(OGP). The proliferation of cultured splenocytes, thymocytes and mesenteric lymph node cells were enhanced by the addition of OGP. Splenic and thymic T lymphocytes, especially TH and Tc cells were significantly increased in OGP-administered mice. OGP markedly increased the production of γ-interferon in mice serum and accelerated the phagocytic activity in peritoneal macrophages. OGP treatment enhanced the apoptosis of L1210 mouse leukemia and Jurkat, Molt4 human leukemia cells, and increased the expression of apoptosis-related ICE, c-myc, p53 gene in Jurkat cell. These results suggest that OGP have an immunoregulatory action and anti-cancer activity.

Keywords

References

  1. 생약학회지 v.10 no.3 오가피의 성분과 약효 한덕룡
  2. Acta. Pharmacol. v.22 Constituents and pharmacological effects of Eucommis and Siberian ginseng Deyama, T.;Nishibe, S.;Nakazawa, Y.
  3. J. Immunol. methods v.65 Rapid colorimetric assay for cellular growth and survival application to proliferation and cytotoxic assays Mosmann, T.
  4. Cell. Immunol. v.12 The differentiation of T lymphocytes, Ⅰ, Proliferation kinetics and interrelationships of subpopulations of mouse thymus cells Shortman, K.;Backson, H
  5. J. Immunol. v.109 Enzyme-linked immunosorbent assay Ⅲ, Quantitation of specific antibodies of enzyme labelled anti-immunoglobulin in antigen-coated tubes Enavall, E.;Perlmann, P.
  6. J. Immunol. Methods v.139 Rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry Nicoletti, I.;Migliorati, G.;Pagliacci, M.C.;Grignani, G.;Riccardi, C.A.
  7. J. Immunol. Methods v.112 Measurement of phagocyte chemiluminescence in a microtiter plate format Blair, A.L.;Cree, I.A.;Beck, J.S.;Hating, M.J.G.
  8. J. Exp. Med. v.181 Reduction in mitochondrial potential constitutes an early irreversible step of programmed lymphocyte death in vivo Zamzami, N.;Marchetti, P.;Castedo, M.;Zanin, C.;Vayssiere, J.L.;Petit, PP.X.;Kroemer, G.
  9. J. Immunol. v.109 Enzyme-linked immunosorbent assay Ⅲ, Quantitation of specific antibodies of enzyme labelled anti-immunoglobulin in antigen-coated tubes Enavall, E.;Perlmann, P.
  10. Statistics for research Dowdy, S.;Weardon, S.
  11. Cellular and molecular immunology v.2 Abbas, A.K.;Lichman, A.H.;Poper, J.S.
  12. J. Immunol. Methods v.174 Chemiluminescence and nitrite determinations by the MALU macrophage cell line Boudard, F.;Vallot, N.;Cabaner, C;Bastide, M.
  13. Br. J. Cancer v.26 Apoptosis, a basic biological phenomenon with wider implications in tissue kinetics Kerr, J.F.K.;Wyllie, A.H.;Currie, A.H.
  14. Int. Rev. Cytol. v.68 The significance of apoptosis. Willie, A.H.;Kerr, J.F.R.;Currie, A.R.
  15. Biochimica et Biophysica Acta v.1133 Analysis and discrimination of necrosis and apoptosis (programmed cell death) by multiparameter flow cytometry Dive, C.;Gregory, C.D.;Phipps, D.J.;Evans, D.L.;Milner, A.E.;Wyllie, A.H.
  16. Radiotherapy & Oncology v.33 no.1 Apoptosis and a re- investigation of the biologic basis for cancer therapy D'Amico, A.V.;McKenna, W.G.
  17. J. Exp. Med. v.179 Purification and characterization of the Fas-ligand that induces apoptosis Suda, T.;Nagata, S.