Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
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Toxic Effect on Phenolic Compound by Colorimeteric Assay in Normal NIH 3T3 Fibroblasts

  • Jin Byung-Jo (Department of Oral Anatomy, College of Dental Medicine, Wonkwang University) ;
  • Lee Joo-Hyun (Department of Oral Anatomy, College of Dental Medicine, Wonkwang University) ;
  • Choi Ki-Wook (School of Medicine, Wonkwang University) ;
  • Lee Jae-Kyoo (School of Medicine, Wonkwang University) ;
  • Han Du-Seok (Department of Oral Anatomy, College of Dental Medicine, Wonkwang University)
  • Published : 2004.09.01
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Abstract

This study was carried out to evaluate the cytotoxic effect of phenolic compound on normal NIH 3T3 fibrolasts. The colorimetric assay for phenol compound, syringic acid was performed by MTT assay or XTT assay. MTT or XTT assays are known as a very sensitive method in measuring the cytotoxic effect of chemical agents in vitro. In the present study, syringic acid on normal Nlli 3T3 fibroblasts did not show any cytotoxicity for MTT assay or XTT assay compared with control after cells were treated with various concentrations of syringic acid for 48 hours. MTT/sub 50/ and XTT/sub 50/ were 3,340.9 μM and 2,462.4 μM of syringic acid, respectively. From the above the results, it is suggested that phenolic compound of syringic acid did not have any cytotoxicity on normal NIH 3T3 fibroblasts.

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