대한한의학회지 (The Journal of Korean Medicine)
- 제25권4호
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- Pages.108-120
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- 2004
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- 1010-0695(pISSN)
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- 2288-3339(eISSN)
Inhibition of Cell Growth and Induction of Apoptosis by Euonymus Alatus (Thunb.) Sieb in Human Leiomyomal Smooth Muscle Cells
- Kim Yi-Geun (Department of Gynecology, College of Oriental Medicine, Dongguk University) ;
- Han Ji-Young (Department of Gynecology, College of Oriental Medicine, Dongguk University) ;
- Park Young-Soo (Department of Gynecology, College of Oriental Medicine, Dongguk University) ;
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Kim Dong-Il
(Department of Gynecology, College of Oriental Medicine, Dongguk University) ;
- Lee Tae-Kyun (Dr. Lee′s Traditional Korean Medical Clinic)
- 발행 : 2004.12.01
초록
Objective : Euonymus alatus (Thunb.) Sieb (EA) is a traditional Korean herbal medicine, commonly used to treat tumors in Korea and China for centuries. Several earlier studies have indicated that EA exhibits anti-tumor properties, but its mechanism remains to be elucidated. In this study, we evaluated the molecular mechanism of EA in a human uterine leiomyomal smooth muscle cell (ULSMC) line. Methods : This study was evaluated by: (a), morphological changes by using acridine orange/ethidium bromide staining; (b), DNA fragmentation by TdT-mediated dUTP nick end labeling (TUNEL); and (c), sub-G1 cell analysis. Results : This study observed that EA treatment caused apoptotic cell death and depletion of intracellular glutathione (GSH) and that reduction of mitochondrial membrane potential was found to be involved in the initiation of apoptosis by EA. Conclusion : This results show that EA exerted clear cytotoxic effects and strongly inhibited the proliferation of ULSMC.
키워드