ALGAE

  • 제19권1호
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  • Pages.59-68
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  • 2004
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  • 1226-2617(pISSN)
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  • 2093-0860(eISSN)
한국조류학회(藻類) (The Korean Society of Phycology)
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Enzymatic Hydrolysis for Effective Extraction of Antioxidative Compounds from Hizikia fusiformis

  • 발행 : 2004.03.31
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초록

Hizikia fusiformis hydroysates by five carbohydrases (Viscozyme, Celluclast, Termamyl and Ultraflo) and five proteases (Protamex, Kojizyme, Neutrase, Flavourzyme and Alcalase) were investigated for their extraction efficacy (yield and total total polyphenolic content) and antioxidative activity (DPPH radical and hydrogen peroxide scavenging activity). Termamyl and Ultraflo of the carbohydrases and Flavourzyme and Alcalase of proteases were selected by their high eficacy of extraction and antioxidative activity. Selected enzymes were used to investigate the optimum enzymatic reaction time and dosage (enzyme/substrate ratio) suitable for hydorolysis. Optimum reaction time for the enzymatic hydrolysis was 3 days and optimum dosage of hydrolysis was observed as 5%. Simultaneously, Ultraflo of the two carbohydrases and Alcalse of the two proteases were selected as the most effective enzymes. Combination of Ultraflo and Alcalase under optimum hydrolysis conditions could intensify the extraction efficacy of antioxidative materials form H. fusiformis. The hydrolysate obtained by combining the enzymes was separated into four different molecular weight fractions (<1kD, 1-10 kD, 10-30 kD and >30 kD) and recorded the polyphenolic content distribution and respective antioxidative ability. The fraction <1kD was identified as less effective and those fractions > 1kD indicated comparatively higher antioxidative activities related to their polyphenolic content.

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참고문헌

  1. Ahn C.B., Jeon Y.J., Kang D.S. Shin T.S. and Jung B.M. 2003. Free radical scavenging activity of enzymatic extracts from brown seaweed Scytosiphon lomentaria by electron spin resonance spectrometry. Food Res. Int. (in press)
  2. Athukorala Y., Lee K.W., Song C, Ahn CB., Shin T.S., Cha Y. J., Shahidi F. and Jeon Y.J. 2003. Potential antioxidant activity of marine red alga Grateloupia filicina extracts. J. Food Lipids 10: 251-263 https://doi.org/10.1111/j.1745-4522.2003.tb00019.x
  3. Beress A., Wassermann O., Tahhan S., Bruhn T., Beress L., Kraiselburd E.N., Gonzalez L.V., Motta G.E. and Chavez P.I. 1993. A new procedure for the isolation of anti-HIV compounds (polysaccharides and polyphenols) from the marine alga Fucus vesiculosus. J. Nat. Prod. 56: 478-488 https://doi.org/10.1021/np50094a005
  4. Brand-Williams W. 1995. Use of a free radical method to evaluate antioxidant activity. Food Sci. Technol. (London) 28: 25-30
  5. Burtin P. 2003. Nutritional value of seaweeds. Electron. J. Environ. Agric. Food Chem. (http://ejeafeche.uvigo.es/2(4)2003/017242003F.htm)
  6. Chandler S.F. and Dodds J.H. 1993. The effect of phosphate, nitrogen and sucrose on the production of phenolics and solasidine in callus cultures of Solanum laciniatum. Plant Cell Reports 2: 105-110 https://doi.org/10.1007/BF00270178
  7. Chiang W.D., Shih C.J. and Chu Y.H. 1999. Functional properties of soy protein hydrolysate produced form a continuous membrane reactor system. Food Chern. 65: 189-194 https://doi.org/10.1016/S0308-8146(98)00193-9
  8. Durig J., Bruhn T., Zurborn K.H., Gutensohn K, Bruhn H.D. and Beress L. 1997. Anticoagulant fucoidan fractions from Fucus vesiculosus induce platelet activation in vitro. Thromb. Res. 85: 479-491 https://doi.org/10.1016/S0049-3848(97)00037-6
  9. Duval B., Shetty K. and Thomas W.H. 2000. Phenolic compounds and antioxidant properties in the snow alga Chlamydomonas nioalis after exposure to UV light. J. App. Phycol. 11: 559-566 https://doi.org/10.1023/A:1008178208949
  10. Fleurennce J. 1999. The enzymatic degradation of algal cell walls: a useful approach for improving accessability. J. App. Phycol. 11: 313-314 https://doi.org/10.1023/A:1008183704389
  11. Heo S.J., Lee KW., Song C.B. and Jeon Y.J. 2003. Antioxidant activity of enzymatic extracts from brown seaweeds. Algae 18: 71-81 https://doi.org/10.4490/ALGAE.2003.18.1.071
  12. Ireland CM., Copp B.R., Foster M.P., Mcdonald L.A., Radisky D.C and Swersey J.C 1993. Biomedical potential of marine natural products. In: Attaway D.H. and Zaborsky O.R. (eds), Marine Biotechnology. Parmaceuiical and Bioactiue Natural Products. Vol. 1. Plenum press, New York. pp. 1-43
  13. Jeon Y.J., Byun H.G. and Kim S.K 2000. Improvement of functional properties of cod frame protein hydrolysates using ultrafiltration membranes. Process Biocnem. 35: 471-478
  14. Kim K.I., Seo H.D., Lee H.S., Cho H.Y. and Yang H.C. 1998. Studies on the blood anticoagulant polysaccharide isolated from hot water extracts of Hizikia [usiforme. Korean J. Food Sci. Nutri. 27: 1204-1210
  15. Kloareg B. and Quatrano R.S. 1988. Structure of cell walls of marine algae and ecophysiological functions of the matrix polysaccharides. Oceanogr. Mar. Biol. Annu. Rev. 26: 259-315
  16. Lahaye M. and Kaeffer B. 1997. Seaweed dietary fibers: structure, physiochemical and biological properties relevant to intestinal physiology. Sci. Aliments. 17: 563-584
  17. Lim S.N., Cheung P.C.K., Ooi V.E.C and Ang P.O. 2002. Evaluation of antioxidative activity of extracts from Brown Seaweed, Sargassum siliquastrum, J. Agric. Food Chem. 50: 3862-3866 https://doi.org/10.1021/jf020096b
  18. Mabeau S. and Kloareg 1987. Isolation and analysis of the cell of brown algae: Fucus spiralis, F. ceranodies, F. serratus, Bifurcario bifuracata and Laminaria digita. J. Exp. Bot. 194: 1573-1580
  19. Moen E., Horn S. and Ostgaard 1997. Biological degradation of Ascopliulum nodosum. J. App. Phycol. 9: 347-357 https://doi.org/10.1023/A:1007988712929
  20. Muller H.E. 1995. Detection of Hydrogen peroxide produced by microorganism on ABTS-peroxidase medium. Zentralbl Bakterio. Mikrobio. Hyg. 259: 151-158
  21. Nagai T. and Suzuki N. 2000. Isolation of collagen from fish waste material-skin, bone and fins. Food Chem. 68: 277-281 https://doi.org/10.1016/S0308-8146(99)00188-0
  22. Nagai T. and Yukimoto T. 2003. Preparation and functional properties of beverages made from sea algae. Food Chern, 80: 327-332
  23. Nardella A., Chaubet F., Boisson-Vidal C, Blondin C, Durand P. and Jozefonvicz J. 1996. Anticoagulent low molecular weight fucans produced by radical process and ion exchange chromatography of high molecular weight fucans extracted from the brown seaweed Ascophyllum nodosum. Carbohy. Res. 289: 201-208 https://doi.org/10.1016/0008-6215(96)00110-3
  24. Okai Y., Okai K.H., Yano Y. and Otani S. 1996. Identification of antimutagenic substances in an extract of edible red alga, Porphura ienera (Asakusa-nori). Cancer letters 100: 235-240 https://doi.org/10.1016/0304-3835(95)04101-X
  25. Ostgaard K 1993. Determination of alginate composition by a simple enzymatic assay. Hydrobiologia 260/261: 513-520 https://doi.org/10.1007/BF00049064
  26. Ragan M.A, Glombitza KW. 1986. Phlorotannins, brown algal polyphenols. In Round F.E. and Chapman D.J. (eds) Progress in Phycological Research, Biopress, Bristol, England. pp.129-241
  27. Ramos E.A.P. and Xiong Y.L. 2002. Antioxidant Activity of Soy Protein Hydrolysates in a Liposomal System. J. of Food Sci., 67: 2952-2956 https://doi.org/10.1111/j.1365-2621.2002.tb08844.x
  28. Ruperez P., Ahrazem O. and Leal J.A. 2002. Potential antioxidant capacity of sulfated polysaccharides from the edible marine brown seaweed Fucus vesiculosus. J. Agric. Food Chem. 50: 840-850 https://doi.org/10.1021/jf010908o
  29. Ruperez P. and Saura-Calixto F. 2001. Diatory fibers and physicochemical properties of edible seaweeds. Eur. Food Res Technol. 212: 349-354 https://doi.org/10.1007/s002170000264
  30. Siriwardhana N., Lee K.W., Kim S.H., Ha J.W. and Jeon Y.J. 2003a. Antioxidant activity of Hizikia Jusiformis on reactive oxygen species scavenging and lipid peroxidation inhibition. Food Sci. Technol. Int. 9: 339-347 https://doi.org/10.1177/1082013203039014
  31. Siriwardhana N., Lee K.W., Kim S.H., Ha J.W. and Jeon Y.J. 2003b. Lipid peroxidation inhibitory effect of Hizikia fusiformis methanolic extract on fish oil and linoleic acid. Food Sci. Technol. Int. (in press)
  32. Yan X., Chuda Y., Suzuki M. and Nagata T. 1999. Fucoxanthin as the major antioxidant in Hijikia fusiformis, a common edible seaweed. Biosci. Biotechnol. Biochem. 63: 605-607 https://doi.org/10.1271/bbb.63.605

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  1. Protective effects of a polysaccharide from Hizikia fusiformis against ethanol toxicity in rats vol.47, pp.1, 2009, https://doi.org/10.1016/j.fct.2008.10.026
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  3. Effect of enzyme-assisted extract of Sargassum coreanum on induction of apoptosis in HL-60 tumor cells vol.24, pp.4, 2012, https://doi.org/10.1007/s10811-011-9685-0
  4. Optimisation of hydrophilic antioxidant extraction fromHizikiafusiformisby integrating treatments of enzymes, heat and pH control vol.43, pp.4, 2008, https://doi.org/10.1111/j.1365-2621.2006.01485.x
  5. Optimization of crude polysaccharides extraction from Hizikia fusiformis using response surface methodology vol.82, pp.1, 2010, https://doi.org/10.1016/j.carbpol.2010.04.029
  6. Effects of Water-Soluble Polysaccharides from Tott on Lipid Absorption and Animal Body Weight vol.42, pp.4, 2013, https://doi.org/10.3746/jkfn.2013.42.4.556
  7. Protective effect of a polysaccharide from Hizikia fusiformis against ethanol-induced cytotoxicity in IEC-6 cells vol.24, pp.1, 2010, https://doi.org/10.1016/j.tiv.2009.08.031
  8. Immunomodulatory Effects of an Enzymatic Extract from Ecklonia cava on Murine Splenocytes vol.10, pp.3, 2008, https://doi.org/10.1007/s10126-007-9062-9
  9. Development of Hijiki-based Edible Films Using High-pressure Homogenization vol.44, pp.2, 2012, https://doi.org/10.9721/KJFST.2012.44.2.162
  10. Characterization of mannanase from Bacillus sp., a novel Codium fragile cell wall-degrading bacterium 2017, https://doi.org/10.1007/s10068-017-0210-3
  11. Isolation and Purification of an Anticoagulant from Schizymenia dubyi by Fermentation vol.13, pp.5, 2007, https://doi.org/10.1177/1082013207085689
  12. Enzymatic Hydrolysis of Plants and Algae for Extraction of Bioactive Compounds vol.29, pp.4, 2013, https://doi.org/10.1080/87559129.2013.818012
  13. Purification and characterization of angiotensin I-converting enzyme inhibitory peptide from enzymatic hydrolysates of Styela clava flesh tissue vol.47, pp.1, 2012, https://doi.org/10.1016/j.procbio.2011.10.005
  14. Evaluation of the Antioxidant Properties of Pediastrum duplex and Dactylococcopsis fascicularis Microalgae vol.13, pp.1, 2010, https://doi.org/10.5657/fas.2010.13.1.018
  15. Antioxidant Properties of Seven Cultivated and Natural Edible Seaweed Extracts from Taiwan vol.21, pp.3, 2012, https://doi.org/10.1080/10498850.2011.594211
  16. Ultrasound-Assisted Aqueous Enzymatic Extraction of Oil from Pomegranate Seeds 2018, https://doi.org/10.1007/s12649-016-9740-9
  17. Chemical Characterization of Enteromorpha prolifera Extract Obtained by Enzyme-Assisted Extraction and Its Influence on the Metabolic Activity of Caco-2 vol.18, pp.3, 2017, https://doi.org/10.3390/ijms18030479
  18. Microwave hydrodiffusion and gravity processing of Sargassum muticum vol.49, pp.6, 2014, https://doi.org/10.1016/j.procbio.2014.02.020
  19. Effect of a glycoprotein from Hizikia fusiformis on acetaminophen-induced liver injury vol.46, pp.11, 2008, https://doi.org/10.1016/j.fct.2008.08.032
  20. Total phenolic content and biological activities of enzymatic extracts from Sargassum muticum (Yendo) Fensholt 2017, https://doi.org/10.1007/s10811-017-1086-6
  21. Processing Optimization of Gelatin from Rockfish Skin Based on Yield vol.13, pp.1, 2010, https://doi.org/10.5657/fas.2010.13.1.001
  22. Effect of UV-B Radiation on GSH and GSH-Related Enzymes of Brown Alga Sargassum thunbergii (Mert.) O.Kuntze vol.518-523, pp.1662-8985, 2012, https://doi.org/10.4028/www.scientific.net/AMR.518-523.5442
  23. Seaweed Bioactive Compounds against Pathogens and Microalgae: Potential Uses on Pharmacology and Harmful Algae Bloom Control vol.16, pp.2, 2018, https://doi.org/10.3390/md16020055
  24. Antioxidant and anti-inflammatory functionality of ten Sri Lankan seaweed extracts obtained by carbohydrase assisted extraction pp.2092-6456, 2018, https://doi.org/10.1007/s10068-018-0406-1