Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Inhibitory Effects of Moutan Cortex Radicis Extracts and Paeonol on Rabbit Platelet Aggregation

  • Lee, Kyung-Sup (College of Pharmacy, Chungbuk National University) ;
  • Oh, Ki-Wan (College of Pharmacy, Chungbuk National University) ;
  • Bae, Ki-Hwan (College of Pharmacy, Chungnam National University) ;
  • Kim, Young-Ho (College of Pharmacy, Chungnam National University) ;
  • Lee, Mi-Yea (College of Social Sciences, Cheongju University) ;
  • Cho, Mi-Ra (College of Pharmacy, Chungbuk National University) ;
  • Jin, Yong-Ri (College of Pharmacy, Chungbuk National University) ;
  • Yun, Yeo-Pyo (College of Pharmacy, Chungbuk National University)
  • Published : 2004.09.01
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Abstract

The present study was undertaken to investigate the effects of Moutan Cortex Radicis extracts and paeonol, a major component, on rabbit platelet aggregation and thromboxane (TX) $A_2$ formation. Moutan Cortex Radicis methanol and butanol layers (100 ${\mu}g/mL$) showed the weak inhibitions, and water layer (100 ${\mu}g/mL$) had no effect on the collagen-induced platelet aggregation. Whereas, hexane and EtOAc layers potently inhibited the collagen (3 ${\mu}g/mL$)-induced platelet aggregation with the $IC_{50}$ values of 10.9${\pm}$1.0 and 31.5${\pm}$0.8 ${\mu}g/mL$, respectively. Paeonol isolated from the hexane-acetone layer specifically inhibited the collagen-induced platelet aggregation with the $IC_{50}$ value of 113.1 ${\pm}$ 0.9 ${\mu}M$, whereas it had little effects on the other agonists such as AA-, thrombin-, A23187- and thapsigargin-induced platelet aggregations. Paeonol also potently inhibited the collagen-induced TXB formation in rabbit platelet in a concentration-dependent manner. These results suggest that paeonol may inhibit rabbit platelet aggregation by interfering with an essential step in collagen-induced platelet activation and $TXA_2$ formation. Paeonol may be a promising candidate for an antiplatelet agent.

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References

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