Korean Journal of Organic Agriculture (한국유기농업학회지)
- Volume 12 Issue 4
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- Pages.451-461
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- 2004
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- 1229-3571(pISSN)
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- 2287-819X(eISSN)
Method Discrimination for Product Traceability and Identification of Korean Native Chicken using Microsatellite DNA
초위성체를 이용한 한국 재래닭의 원산지 추적 및 개체 식별 방법에 관한 연구
- Park, Mi-Hyun ;
- Oh, Jae-Don ;
- Jeon, Gwang-Joo ;
- Kong, Hong-Sik ;
- Sang, Byong-Don ;
- Choi, Chull-Hwan ;
- Yeon, Sung-Hum ;
- Cho, Byong-Wok ;
- Lee, Hak-Kyu
- 박미현 (한경대학교 유전정보연구소) ;
- 오재돈 (한경대학교 유전정보연구소) ;
- 전광주 (한경대학교 유전정보연구소) ;
- 공홍식 (축산물등급판정소) ;
- 상병돈 (축산연구소) ;
- 최철환 (축산연구소) ;
- 연성흠 (축산연구소) ;
- 조병욱 (밀양대학교) ;
- 이학교 (밀양대학교)
- Published : 2004.12.01
Abstract
In an animals, identification system has been widely used by ear tag with dummy code and blood typing for parernity. Also, genotyping methods were using for useful mean of individual identification for live animals. In the case of genotyping estimation of gene in population of korean native chicken. In this study, we tested for development of genetic markers used it possible to determination of individual identification system. The candidate genetic markers were used already bow 10 of microstalite DNA sequence information in chromosome No. 1 and 14. Result of analysis for genotyping, the number of alleles of those microstatelites DNA was shown minimal 3 to 12 and the heterozygote expression frequency range was shown from 0.617 to 0.862. In our result, effective number of allele for each microsatellites DNA was shown 3~7, and the accuracy of individual identification was shown nearly 100%, when used with 6 genetic marker. This study was about genotyping method for identification used specific genetic marker form microsatellite DNA in the brand marketing of korean native chicken. Our results suggest that genotyping method used specific genetic marker from microsatellite DNA might be very useful for determination of individual identification.
Keywords
- Individual identification in live animals system;
- Microsatellite DNA;
- Individual traceability of origin;
- DNA test;
- Distribution of chicken