Plant Resources
- Volume 7 Issue 3
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- Pages.163-169
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- 2004
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- 1226-8771(pISSN)
Isolation and Transcriptional Expression of CuZn Superoxide Dismutase from Codonopsis lanceolata
- Lee, Kang (Biopia Co., Ltd) ;
- In, Jun-Gyo (Biopia Co., Ltd) ;
- Yu, Chang-Yeon (College of Agriculture & Life Sci., Kangwon Natl. Univ.,) ;
- Yun, Song-Joong (Faculty of Biological Research Sci., Chonbuk Natl. Univ.,) ;
- Min, Byung-Hoon (Division of Horticulture & Landscape Architecture, Pai-Chai Univ.,) ;
- Rho, Yeong-Deok (College of Life Science & Center for Oriental Medicinal Materials and Processing Kyung Hee University) ;
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Kim, Moo-Sung
(College of Life Science & Center for Oriental Medicinal Materials and Processing Kyung Hee University) ;
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Yang, Deok-Chun
(College of Life Science & Center for Oriental Medicinal Materials and Processing Kyung Hee University)
- Published : 2004.12.01
Abstract
To investigate the defense mechanism against the abiotic stress, a cDNA clone encoding a CuZn superoxide dismutase (CuZnSOD) protein was isolated from a cDNA library prepared from tabroot mRNAs of Codonopsis lanceolata. The eDNA, designated ClSODCc, is 799 nucleotides long and has an open reading frame of 459 bp with a deduced amino acid sequence of 152 residues. The deduced amino acid sequence of ClSODCc matched to the previously reported CuZnSODs. Consensus amino acid residues (His-45, -47, -62, -70, -79, -119 and Asp-82) were involved in Cu-, Cu/Zn-, and Zn- binding ligands. The deduced amino acid sequence of ClSODCc showed high homologies (82%-86%) regardless of species. Expression of ClSODCc by oxidative stress was increased up to 1 h after treatment and declined gradually. Much earlier and stronger expression of ClSODCc was observed in the cold stress treatment.