Environmental Analysis Health and Toxicology

The Korean Society of Environmental Health and Toxicology (환경독성보건학회)
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Cadmium Altered Gene Expression Related to Zinc Homeostasis in the Mouse Brain

카드뮴이 마우스 뇌에서 아연의 항상성에 관여하는 유전자발현에 미치는 영향

  • 박종안 (순천향대학교 환경보건학과) ;
  • 여은영 (순천향대학교 환경보건학과) ;
  • 남상훈 (순천향대학교 환경보건학과) ;
  • 장봉기 (순천향대학교 환경보건학과) ;
  • 이종화 (순천향대학교 환경보건학과) ;
  • 김완종 (순천향대학교 생물학과)
  • Published : 2004.12.01
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Abstract

Metallothionein (MT), a small protein molecule which can bind or release metal ions, is involved in the regulation of cellular metal homeostasis. This study was investigated the accumulation of cadmium in blood, tissue (liver, kidney and brain), and the effect of cadmium on several key genes (MT-I, MT-II, ZnT-1) in zinc metabolism in the mouse. Mouses weighing 20∼25 g were randomly assigned to control and cadmium treated group (Cd group). Cd group was intraperitoneally injected with cadmium 2, 4, 8 mg/kg and control group was administerd with saline. Mouses of each group were sacrificed by decapitation 4 hours after the administration of cadmium. Cadmium contents in blood, liver, kidney and brain were increased by a dose-dependent manner. Accumulation of cadmium was mainly occurred in liver and kidney. Induction of MT-I and MT-II protein was increased, but ZnT-1 expression was decreased in a dose-dependent manner by the treatment of 2∼8 mg/kg cadmium. These results suggested that cadmium can be transported to brain and alter the expression of several key genes in zinc homeostasis.

Keywords

References

  1. 김남송. 전처치 기간에 따른 흰쥐의 조직내 카드뮴 및 metallothionein 생성에 관한 연구. Korean J Toxicol 1997; 13(1-2): 103-110
  2. 김판기. 카드뮴이 랫드에 미치는 독성학적 변화에 관한 연구. 용인대학교 논문집 1997; 13: 507-510
  3. 박종안, 오혜정, 이종화. 흑연로 원자흡수분광법에 의한 혈액 중 카드뮴 정량 분석. 한국산업위생학회지 1996; 6(2): 301-312
  4. 이순재. 급성 카드뮴 중독 흰쥐 간조직에서의 유리기 생성계의 활성화와 조직손상. 대구효성카톨릭대학교 석사학위논문집 1998; 58: 240-242
  5. 임순혁. Cadmium 투여에 의한 흰쥐의 간, 신장 및 혈액에서의 축적에 관한 연구. 청주대학교 석사학위논문.; 1992. pp. 5-6
  6. Abuja PM and Albertini R. Methods for monitoring Oxida-tive stress, lipid peroxidation and oxidation resistance of lipoproteins. Clin Chim Acta 2001; 306(1-2): 1-7 https://doi.org/10.1016/S0009-8981(01)00393-X
  7. Adams JD Jr. Klaidman LK, Chang ML and Yang J. Brain oxidative stress-analytical chemistry and thermody-namics of glutathione and NADPH. Curr Top Med Chem 2001; 1(6): 473-482 https://doi.org/10.2174/1568026013394778
  8. Angelo A Valois and William S Webster. The choroid plexus as a target site for cadmium toxicity following chronic exposure in the adult mouse. An ultrastructural study. Toxicology 1989; 55(1-2): 193-205 https://doi.org/10.1016/0300-483X(89)90186-8
  9. Arlt S, Kontush A, Muller-Thomsen T and Beisiegel U. Lipid peroxidation as a common pathomechanism in Cor-onary heart disease and Alzheimer's disease. Gerontol Geriatr 2001; 34(6): 461-465 https://doi.org/10.1007/s003910170019
  10. Arranz MA, Perez MI, Saiz-VadiIlo MC, Basagoiti I, Katarneh SM, Ribas B and Cadorniaa R. Pharmaco-kinetics of cadmium-109 in blood and brain structure of the rat. Rev Esp 1984; 40(3): 365-370
  11. Arvidson B and Tjalve H. Distribution of 109Cd in the nervous system of rats after intravenous injection. Acta Neuropathol(Berl) 1986; 69(1-2): 111-116 https://doi.org/10.1007/BF00687046
  12. Atsushi Takeda, Sachiyo Takefuta, Hiromi Ijiro, Shoji Okada and Naoto Oku. 109Cd transport in rat brain. Brain Research Bulletin 1999; 49(6): 453-457 https://doi.org/10.1016/S0361-9230(99)00080-5
  13. Berg JW and Burbank F. Correlation between carcinogenic trace metals in water supplies and cancer mortality. Ann N.Y Acad Sci.; 1972. pp. 249-262
  14. Cantilena LR and Klaassen CD. Comparison of the effecti-veness of several chelators after single administration on the toxicity, distribution and excretion of cadmium. Toxicol Appl Pharmacol 1981; 58: 452-460 https://doi.org/10.1016/0041-008X(81)90098-3
  15. Choudhuri S, McKim JM Jr and Klaassen CD. Differential expression of the metallothionein gene in liver and brain of mice and rats. Toxicol appl Pharmacol 1993; 119: 1-10 https://doi.org/10.1006/taap.1993.1037
  16. Clausen J and Rastogi SC. Heavy metal pollution among autoworkers, lead. Bri J Ind med 1977; 34: 208-215
  17. Coyle P, Philcox JC, Carey LC and Rofe AM. Metallothionein: The multipurpose protein. CMLS Cell Mol Life Sci 2002; 59: 627-647 https://doi.org/10.1007/s00018-002-8454-2
  18. Cuthbert OS. Metallothionein in human disease. Cellular and Molecular Biology 2000; 46(2): 465-488
  19. Deaven LL and Campbell EW. Factors affecting the induc-tion of chromosomal aberrations by cadmium in Chinese hamster cells. Cytogenet Cell Genet 1980; 26: 251-260 https://doi.org/10.1159/000131446
  20. Dudley RE, Svovoda DJ and Klaassen CD. Acute exposure to cadmium causes severe liver injury in rats. Toxicol Appl Pharmacol 1982; 65: 302-313 https://doi.org/10.1016/0041-008X(82)90013-8
  21. Evans J and Hastings L. Accumulation of Cd (II) in the CNS depending on the route of administration: intraperitoneal, intratracheal, or intranasal. Fundam Appl Toxicol Aug 1992; 19(2): 275-278 https://doi.org/10.1016/0272-0590(92)90161-A
  22. Faeder EJ, Chanet SQ and King LC. Biochemical and uItras-tructural changes in livers of cadmium treated rats. Toxi-col Appl Pharmacol 1977; 39: 473-483 https://doi.org/10.1016/0041-008X(77)90139-9
  23. Figueiredo-Pereira ME and Cohen G. The ubiquitin/ Proteasome pathway: friend or foe in zinc-, cadmium-, and $H_2O_2$-induced neuronal oxidative stress. Mol Biol Rep 1999; 26(1-2): 65-69 https://doi.org/10.1023/A:1006909918866
  24. Glabe C. Intracellular mechanisms of amyloid accumulation and pathogenesis in Alzheimer's disease. Mol Neurosci 2001; 17(2): 137-145 https://doi.org/10.1385/JMN:17:2:137
  25. Goyer RA. Toxic effects of metals. In: Klassen CD, Amdur MO and Doull J (eds.), Casarett and Doull's Toxicology., 5th Ed., Macmillan Publishing Co., New york.; 1996. pp. 699-702
  26. Grundman M. Vitamin E and Alzheimer disease: The basis for additional clinical trials. Clin Nutr 2000; 71(2): 630-636
  27. Hammond PB and Foulkes EC. Metal ion toxicity in man and animals, In: Siegel, H.(ed.), Metal Ions in Biological Systems. Marcel Dekker, New York 1994; 20: 177-182
  28. K$\"a$gi JHR and Schaffer A. Biochemistry of metallothionein. Biochemistry 1988; 27: 8509-8515 https://doi.org/10.1021/bi00423a001
  29. Kanski J, Varadarajan S, Aksenova M and Butterfield DA. Role of glycine-33 and methionine-35 in Alzheimer's amyloid beta-peptide l-42-associated oxidative stress and neurotoxicity. Biochim Biophys Acta 2002; 1586 (2): 190-198 https://doi.org/10.1016/S0925-4439(01)00097-7
  30. Karelson E, Bogdanovic N, Garlind A, Winblad B, Zilmer K, Kullisaar T, Vihalemm T, Kairane C and Zilmer M. The cerebrocortical areas in normal brain aging and in Alzheimer's disease: noticeable differences in the lipid peroxidation level and in antioxidant defense. Neuro-chem Res 2001; 26(4): 353-361
  31. Kazantizis G. Renal tubular disfunction and abnormalities of calcium metabolism in cadmium workers. Environ Health Perspect 1979; 28: 155 https://doi.org/10.2307/3428918
  32. Keen CL and Hurley LS. Zinc and reproduction: Effects of deficiency on fetal and postnatal development, in Zinc and Human Biology. Springer-Verlag London.; 1989. pp. 183-200
  33. Kolonel L and Winkelstein W Jr. Cadmium and prostatic carcinoma. Lancet 1977; 2: 566-567
  34. Kumar R, Agarwal A and Seth PK. Oxidative stress-medi-ated neurotoxicity of cadmium. Toxicol Letters 1996; 89: 65-69 https://doi.org/10.1016/S0378-4274(96)03780-0
  35. Langmade SJ, Ravindra R, Daniels PJ and Andrews GK The transcription factor MTF-l mediates metal regulation of the mouse ZnTl gene. J Biol Chem 2000; 275: 34803-34809 https://doi.org/10.1074/jbc.M007339200
  36. Lui E, Fisman M, Woung C and Diaz F. Metals and the liver in Alzheimer's disease. An investigation of hepatic zinc, copper, cadmium, and metallothionein. Am Geriatr Soc 1990; 38(6): 633-639 https://doi.org/10.1111/j.1532-5415.1990.tb01421.x
  37. Malcolm D. Cadmium as a carcinogen, In: Cadmium toxi-city. MennearJH.; 1979. pp. 173-180
  38. Math PC and Gordon JL. Zinc metabolism in the brain relevance to human neurodegenerative disorders. Neuro-biology of Disease 1997; 4: 137-169 https://doi.org/10.1006/nbdi.1997.0163
  39. McMahon RJ and Cousins RJ. Regulation of the zinc trans-porter ZnT-1 by dietary zinc. Proc Natl Acad Sci 1998; 95: 4841-4846 https://doi.org/10.1073/pnas.95.9.4841
  40. O'Riordan ML, Hughes EG and Evans HJ. Chromosome studies on blood lymphocytes of men occupationally exp-osed to cadmium. Mutat Res 1978, 58: 305-311 https://doi.org/10.1016/0165-1218(78)90023-X
  41. Palmitier RD, Findley SD, Whitmore TE and Durnam DM. MT-III, a brain-specific member of the metallothionein gene family. Proc Natl Acad Sci 1992; 89: 6333-6337 https://doi.org/10.1073/pnas.89.14.6333
  42. Panayi Ae, Spyrou NM, Iversen BS, White MA and Part P. Determination of cadmium and zinc in Alzheimer's brain tissue using inductively coupled plasma mass Spectrometry. Neurol Sci 2002; 195(1): 1-10 https://doi.org/10.1016/S0022-510X(01)00672-4
  43. Pitchumoni SS, Doraiswamy PM. Current status of antioxi-dant therapy for Alzheimer's Disease. Am Geriatr Soc 1998; 46 (12): 1566-1572 https://doi.org/10.1111/j.1532-5415.1998.tb01544.x
  44. Risso-de Faverney C, Devaux A, Lafaurie M, Girard JP, Bailly B and Rahmani R. Cadmium induces apotosis and genotoxicity in rainbow trout hepatocyte through generation of reactive oxygen species. Aquat Toxicol 2001; 53(1): 65-76 https://doi.org/10.1016/S0166-445X(00)00154-5
  45. Roels HA, Lauwery RR, Buchet JP, Bernard A, Chettle DR, Harrey TC and AI-Haddad IK. In vivo measurement of liver and kidney cadmium in workers exposed to this metal: Its significance with repect to cadmium in blood and urine. Environ Res 1981; 26: 217-240 https://doi.org/10.1016/0013-9351(81)90199-7
  46. Schrauzer GN, White DA and Schneider CJ. Cancer morta-lity correlation studies. IV. Associations with dietary intakes and blood levels of certain trace elements, notal-by Se-antagonists. Bioinorg Chem 1972; 7: 35-36 https://doi.org/10.1016/S0006-3061(00)80127-1
  47. Sendelbach LE and Klaassen CD. Kidney synthesizes less metallothionein than liver in response to cadmium Chlo-ride and cadmium metallothionein. Toxicol Appl Phar-macol 1988; 92: 95-102 https://doi.org/10.1016/0041-008X(88)90231-1
  48. Shaikh ZA and Lucis UJ. Cadmium and zinc binding in mammalian liver and kidneys. Arch Environ health 1972; 24: 419-425 https://doi.org/10.1080/00039896.1972.10666118
  49. Shukla A, Shukla GS and Srimal RC. Cadmium-induced alterations in blood-brain barrier permeability and its possible correlation with decreased microvessel antioxi-dant potential in rat. Human & Experimental Toxicology 1996: 15(5): 400-405 https://doi.org/10.1177/096032719601500507
  50. Snyder WS, Cook MJ, Nasset ES, Karhausen LR, Howells GP and Tipton IH. International Commission on Radio-logical Protection. Report of the task group on reference man. New York. ICRP Publication 23.; 1975
  51. Szuster-Ciesielska A, Stachura A, Slotwinska M, Kaminska T, Sniezko R, Paduch R, Abramczyk D, Filar J and Kan defer-Szerszen M. The inhibitory effect of zinc on Cadi-mium-induced cell apotosis and reactive oxygen species production in cell cultures. Toxicology 2000; 145 (2-3): 159-171 https://doi.org/10.1016/S0300-483X(00)00144-X
  52. Taubeneck MW, Daston GP, Rogers JM and Keen CL. AI-tered maternal zinc metabolism following exposure to diverse developmental toxicants. Repro Toxicol 1994; 8: 25-40 https://doi.org/10.1016/0890-6238(94)90064-7
  53. Taubeneck MW, Daston GP, Rogers JM, Gershwin ME, Ansari A and Keen CL. Tumor necrosis factor-alpha alters maternal and embryonic zinc metabolism and is developmentally toxic in mice. J Nutr 1995; 125: 908-919
  54. Uchida Y, Takio K, Titani K, Ihara Y and Tomonaga M. The growth inhibitory factor that is deficient in the AIzheimer's disease brain is a 68 amino acid metallothionein-like protein. Neuron 1991; 7: 337-347 https://doi.org/10.1016/0896-6273(91)90272-2
  55. Webster WS. Cadmium induced fetal growth retardation in the mouse. Arch Environ Health 1978; 32: 16-22
  56. WHO (World Health Organization). Cadmium-environ-mental aspects. Environmental Health Criteria 135.; 1992
  57. Wong KL and Klaassen CD. Tissue retention of cadmium in rats during postnatal development. Toxicol Appl Phar-macol 1980; 53: 343-353 https://doi.org/10.1016/0041-008X(80)90435-4
  58. Wong KL and Klaassen CD. Neurotoxic effects of cadmium in young rats. Toxicol Appl Pharmacol 1982; 63: 330-33 https://doi.org/10.1016/0041-008X(82)90261-7
  59. Wren CD, Harris S and Haittrup N. Ecotoxicology of mer-cury and cadmium, In: Handbook of ecotoxicology. Hoff-man DS, Rattner BA, Burton GA, Jr and J caims, Jr (Eds.), CRC Press, Boca Raton.; 1995. pp. 392-423