대한핵의학회지 (The Korean Journal of Nuclear Medicine)

대한핵의학회 (The Korean Society of Nuclear Medicine)
권호 표지

분자영상연구를 위한 분자생물학 기법 소개

Introduction To Basic Molecular Biologic Techniques for Molecular Imaging Researches

  • 강주현 (서울대학교 의과대학 핵의학과, 암연구소 분자영상 및 치료연구실)
  • Kang, Joo-Hyun (Department of Nuclear Medicine and Cancer Research Institute, Seoul National University College of Medicine)
  • 발행 : 2004.04.30
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초록

Molecular imaging is a rapidly growing field due to the advances in molecular biology and imaging technologies. With the introduction of imaging reporter genes into the cell, diverse cellular processes can be monitored, quantified and imaged non-invasively in vivo. These precesses include the gene expression, protein-protein interactions, signal transduction pathways, and monitoring of cells such as cancer cells, immune cells, and stem cells. In the near future, molecular imaging analysis will allow us to observe the incipience and progression of the disease. These will make us easier to give a diagnosis in the early stage of intractable diseases such as canter, neuro-degenerative disease, and immunological disorders. Additionally, molecular imaging method will be a valuable tool for the real-time evaluation of cells in molecular biology and the basic biological studies. As newer and more powerful molecular imaging tools become available, it will be necessary to corporate clinicians, molecular biologists and biochemists for the planning, interpretation, and application of these techniques to their fullest potential. in order for such a multidisciplinary team to be effective, it is essential that a common understanding of basic biochemical and molecular biologic techniques is achieved. Basic molecular techniques for molecular imaging methods are presented in this paper.

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참고문헌

  1. Cohen SN, Chang AC, Boyer HW, Helling RB. Construction of biologically functional bacterial plasmids in vitro. Proc Natl Acad Sci U S A 1973;70:3240-4 https://doi.org/10.1073/pnas.70.11.3240
  2. Kuhnlein U, Linn S, Arber W. Host specificity of DNA produced by Escherichia coli. XI. In vitro modification of phage fd replicative form. Proc Natl Acad Sci U S A 1969;63:556-62
  3. Watson JD, Gilman M, Witkowsi J, Zoller M. Recombinant DNA. 2nd ed. New York: Scientific American Books: 1992. p79-134
  4. Weaver RF. Molecular Biology. 2nd ed. New York: Mc Graw Hill: 2002. p60-90
  5. Lodish H, Berk A, Zipursky SL, Matsudaira P, Baltimore D, Darnell JE. Molecular Cell Biology. 4th ed. New York: WH Freeman and company. 2000. p208-251
  6. Izants JG, Weintraub H. Inhibition of thymidine kinase gene expression by antisense RNA: a molecular approach to genetic analysis. Cell 1984;36:1007-15 https://doi.org/10.1016/0092-8674(84)90050-3
  7. Fire, A., Xu, S., Montgomery, M.K., Kostas, S.A., Driver, S.E., Mello, C.C. Potent and specific genetic interference by doublestranded RNA in Caenorhabditis elegans. Nature 1998;391:806-11
  8. Goeddel DV, Heyneker HL, Hozumi T, Arentzen R, Itakura K, Yansura DG, et al. Direct expression in Escherichia coli of a DNA sequence coding for human growth hormone. Nature 1979;281: 544-8 https://doi.org/10.1038/281544a0
  9. Valenzuela P, Medina A, Rutter WJ, Ammerer G, Hall BD. Synthesis and assembly of hepatitis B virus surface antigen particles in yeast. Nature 1982;298:347-50 https://doi.org/10.1038/298347a0
  10. Hitzeman RA, Leung DW, Perry LJ, Kohr WJ, Levine HL, Goeddel DV. Secretion of human inteferons by yeast. Science 1983;219: 620-5 https://doi.org/10.1126/science.6186023
  11. Luckow VA, Summers MD. Trends in the development of baculovirus expression vectors. Bio/Tech 1988;6:47-55 https://doi.org/10.1038/nbt0188-47
  12. Medin JA, Hunt L, Gathy K, Evans RK, Coleman MS. Efficient, low-cost protein factories; expression of human adenosine deaminase in baculovirus-infected insect larvae. Proc Natl Acad Sci U S A 1990;87:2760-4 https://doi.org/10.1073/pnas.87.7.2760
  13. Fussenegger M, Bailey JE, Hauser H, Mueller PP. Genetic optimization of recombinant glycoprotein production by mammaliancells. Trends Biotechnol 1999;17:35-42 https://doi.org/10.1016/S0167-7799(98)01248-7
  14. Kaplitt MG, Loewy AD. Viral Vectors: Gene Therapy and Neuroscieuce Applications. London: Academic Press: 1995. p173-237
  15. Graham FL. Adenovirus vectors for high-efficiency gene transfer into mammalian cells. Immunol Today 2000;21:426-8 https://doi.org/10.1016/S0167-5699(00)01676-5
  16. Nettelbeck DM. Rivera AA, Balague C, Alemany R, Curiel DT. Novel Oncolytic Adenoviruses Targeted to Melanoma: Specific Viral Replication and Cytolysis by Expression of E1A Mutants from the Tyrosinase Enhancer/Promoter. Cancer Res 2002;62:4663-70
  17. Lamfers MLM. Grill J, Dirven CMF, van Beusechem VW, Geoerger B, van den Berg J. Potential of the Conditionally Replicative Adenovirus Ad5-24RGD in the Treatment of Malignant Gliomas and Its Enhanced Effect with Radiotherapy. Cancer Res 2002;62:5736-42
  18. St George JA, Gene therapy progress and prospects: adenoviral vectors. Gene Ther 2003;10:1135-41 https://doi.org/10.1038/sj.gt.3302071
  19. Daly G, Chernajovsky Y. Recent developments in retroviralmediated gene transduction. Mol Ther 2000;2:423-34 https://doi.org/10.1006/mthe.2000.0211
  20. Buchschacher GL, Wong-Staal F. Development of lentiviral vectors for gene therapy for human diseases. Blood 2000;95:2499-504
  21. Mullis KB, Faloona FA. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods Enzymol 1987;155: 335-50 https://doi.org/10.1016/0076-6879(87)55023-6
  22. Wong C, Dowling CE, Saiki RK, Higuchi RG, Erlich HA, Kazazian HH Jr. Characterization of beta-thalassaemia mutations using direct genomic sequencing of amplified single copy DNA. Nature 1987; 330:384-6 https://doi.org/10.1038/330384a0
  23. Innis MA, Gelfand DH, Sninsky JJ, and White TJ eds. PCR Protocols: A Guide to Methods and Applications. Academic, NY., 1990
  24. Erlich HA, Gelfand D, Sninsky JJ. Recent advances in the polymerase chain reaction. Science 1991;252:1643-51 https://doi.org/10.1126/science.2047872