Plant Resources
- Volume 7 Issue 2
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- Pages.87-92
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- 2004
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- 1226-8771(pISSN)
Application of in vitro ovary culture for cottonwood (Populus deltoides) breeding
- Kang, Ho-Duck (Forest Biotechnology Lab., Department of Forest Resources, Dongguk University) ;
- Lee, Min-Soon (Department of Environment and Forest Products, Joongbu University)
- Published : 2004.08.01
Abstract
Five different poplar hybrids were tested for rescuing embryo to elongate in vitro plantiets after hybridization. Ovaries and ovules were cultured on Woody Plant Medium (WPM) supplemented with cytokinins, 6-benzylamine (BA) and zeatin. Multiple shoots were initiated from half section of capsule with immature embryos after 21 days from pollination and tiny shoots were formed after the expansion of cotyledons in ovule cultures. Germinating response was better in intraspecific hybrids
Keywords
- Embryo rescue;
- immature ovule and ovary culture;
- in vitro germination;
- multiple shoots;
- intra-and interspecific poplar hybrids