Environmental Analysis Health and Toxicology

The Korean Society of Environmental Health and Toxicology (환경독성보건학회)
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Evaluation of Toxic Effects Caused by Pesticides in Escherichia coli Using Recombinant Bioluminescent Bacteria

유전자 재조합 발광박테리아를 이용한 농약 독성평가

  • 김지원 (광주과학기술원(GIST) 환경공학과 환경생물공학연구실) ;
  • 구만복 (한국확학연구원 부설 안전성 평가연구소, 환경독성시험연구부, 생태독성팀)
  • Published : 2004.09.01
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Abstract

본 연구에서는 유전자 재조합 발광 박테리아를 이용하여 농약에 대한 박테리아의 스트레스 반응과 세포 독성을 분석하였다. 15종류의 농약에 대하여 유전자 손상, 생물막 손상, 산화적 손상 및 단백질 손상을 측정할 수 있는 발광 박테리아와 독성 유무로 인한 세포 독성을 측정할 수 있는 발광 박테리아, 5종을 이용하여 스트레스 반응을 분류하고 세포 독성 정토를 분석하였다. 그 결과, 농약의 화학적 구조가 박테리아의 스트레스 반응에 영향을 미치며, 산화과정이 진행 됨에 따라 독성의 작용 기작이 변하는 것을 확인 할 수 있었다. 이와 같은, 유전자 재조합 발광 박테리아를 이용한 생물체내의 독성 메커니즘에 대한 분석은 생태계 유해물질들에 의한 독성을 분석하고 예상하기 위해 적용될 수 있을 것이다.

Keywords

References

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