Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
권호 표지

Partial Cloning of Histone Deacetylase Genes from Ganoderma lucidum.

영지에서 Histone Deacetylase 유전자의 부분 클로닝

  • 김선경 (강원대학교 생명과학부 미생물학과) ;
  • 금주희 (강원대학교 생명과학부 미생물학과) ;
  • 최형태 (강원대학교 생명과학부 미생물학과)
  • Published : 2004.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

Histone deacetylase (HDAC) removes acetyl group in lysine residue of histone protein, which is transferred by histone acetylase. HDAC is important in the stabilization and regulation of gene expression in eukaryotic organisms. PCR has been carried out to clone HDAC genes using cDNA library and genomic DNA as the templates from Ganoderma lucidum isolated in Korea. One 470 bp cDNA gene fragment, and 3 genomic HDAC fragments (585 bp, 589 bp, 630 bp) were amplified. When their deduced amino acid sequences were compared with other fungal HDACs, they showed 59-72％ homology.

염색질을 구성하는 histone 단백질 lysine 잔기에 histone acetylase에 의하여 결합된 acetyl기를 제거하는 histone deacetylase (HDAC)는 진핵세포 생물의 염색질 안정 파 및 유전자 발현에 매우 큰 영향을 미친다. 국내에서 분리된 영지의 HDAC 유전자를 클로닝 하고자 cDNA 및 genomic DNA를 대상으로 PCR을 수행한 결과 470bp의 cDNA유전자와, 585 bp, 589 bp 및 630 bp길이의 genomic DNA유전자 조각을 클로닝 하였다. 이들의 염기서열을 근거로 아미노산 서열을 다른 균류의 HDAC와 비교한 결과 59-72％의 상동성을 보였다.

Keywords

References

  1. Graessle, S., M. Dangl, H. Haas, K. Mair, P. Trojer, E.-M. Brandtner, J. Walton, P. Loidl, and G. Brosch. 2000. Characterization of two putative histone deacetylase genes from Aspergillus nidulans. Biochim. Biophys. Acta 1492, 120-126
  2. Imai, S., C. Armstrong, M. Kaeberlein, and L. Guarente. 2000. Transcriptional silencing and longevity protein Sir2 is an NAD dependent histone deacetylase. Nature 403, 795-800
  3. Jang, I.C., Y.M. Pahk, S.I. Song, H.J. Kwon, B.H. Nahm, and J.K. Kim. 2003. Structure and expression of the rice class-I type histone deacetylase genes OsHDAC1-3: OsHDAC1 overexpression in transgenic plants leads growth rate and altered architecture. Plant J. 33, 531-541
  4. Kim, M.-M., S.-O. Yoon, Y.-S. Cho, and A.-S. Chung. 2004. Histone deacetylases, HDAC1 and HSIR2, act as a negative regulator of aging through p53 in human gingival fibroblast. Mech. Aging Develop. 125, 351-357
  5. Kim, S., J. Song, and H.T. Choi. 2004. Genetic transformation and mutant isolation in Ganoderma lucidum by restriction enzymemediated integration. FEMS Microbiol. Lett. 233, 201-204
  6. Kim, S., Y. Leem, K. Kim, and H.T. Choi. 2001. Cloning of an acidic laccase gene (clac2) from Coprinus congregatus and its expression by external pH. FEMS Microbiol. Lett. 195, 151-156
  7. Ko, E.-M., Y.-E. Leem, and H.T. Choi. 2001. Purification and characterization of laccase isozymes from white-rot basidiomycete Ganoderma lucidum. Appl. Microbiol. Biotechnol. 57, 89- 102
  8. Leem, Y., S. Kim, I.K. Ross, and H.T. Choi. 1999. Transformation and laccase mutant isolation in Coprinus congregatus by restriction enzyme-mediated integration. FEMS Microbiol. Lett. 172, 35- 40
  9. Lusser, A., G. Brosch, A. Loidl, H. Haas, and P. Loidl. 1997. Identification of maize histone deacetylase HD2 as an acidic nucleolar phosphoprotein. Science 277, 88-91
  10. Srikantha, T., L. Tsai, K. Daniels, A. Klar, and D. Soll. 2001. The histone deacetylase genes HDA1 and RPD3 play distinct roles in regulation of high frequency phenotypic switching in Candida albicans. J. Bacteriol. 183, 4614-4625
  11. Taunton, J., C. Hassig, and S. Schreiber. 1996. A mammalian histone deacetylase related to the yeast transcriptional regulator Rpd3p. Science 272, 408-411
  12. Ura, K., H. Kurumizaka, S. Dimitrov, G. Almouzni, and A. Wolffe. 1997. Histone acetylation: influence on transcription, nucleosome mobility and positioning, and linker histone-dependent transcriptional repression. EMBO J. 16, 2096-2107
  13. Wu, K., L. Tian, K. Malik, D. Brown, and B. Miki. 2000. Functional analysis of HD2 histone deacetylase homologues in Arabidopsis thaliana. Plant J. 22, 19-27