Journal of Plant Biotechnology

한국식물생명공학회 (The Korean Society of Plant Biotechnology)
권호 표지

Somatic Embryogenesis in Withania somnifera (L.) Dunal

  • Rani, Gita (Department of Botanical and Environmental Sciences, Guru Nanak Dev University) ;
  • Virk, Gurdip Singh (Department of Botanical and Environmental Sciences, Guru Nanak Dev University) ;
  • Nagpal, Avinash (Department of Botanical and Environmental Sciences, Guru Nanak Dev University)
  • 발행 : 2004.06.01
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초록

Somatic embryos were formed from calli obtained from axillary shoots (raised from nodal segments of glasshouse-grown plants under aseptic conditions), internodal segments (from in vitro-raised plants), and root and coty-ledonary leaf segments (from in vitro-raised seedlings) after 8 weeks of initial culture. Embryo formation was the highest (97.33%) from cotyledonary leaf callus on Mura-shige and Skoog's (MS) medium containing kinetin (KN) (3 mg/L). Somatic embryo induction was lesser with different combinations of auxins while it increased to 100% in internodal segment and cotyledonary leaf calli with 6-benzyladenine (BA) (2mg/L) along with 2,3,5-triiodobenzoic acid (TIBA) (2mg/L). The shoots were induced from somatic embryos raised from root, coty-ledonary leaf and internodal segment calli grown on MS medium containing BA in combination with indole-3-acetic acid (IAA). Maximum of 66.67% cultures formed shoots on MS medium containing BA (1mg/L) in combination with IAA (2mg/L). The shoots raised from somatic embryos were rooted on MS medium supplemented with indole-3-butyric acid (IBA) (2mg/L). The plantlets transferred to the field showed 70% survival rate after one year.

키워드

참고문헌

  1. Ammirato PV, Styer DJ (1985) Strategies for large scale mani-pulation of somatic embryos in suspension cultures. In: Zaitlin M, Day P, Hollaender A (eds), Biotechnology in Plant Science, pp 161-178. Academic Press, New York
  2. Anbazhagan VR, Ganapathi A (1999) Somatic embryogenesis in cell suspension cultures of pigeonpea (Cajanus cajan). Plant Cell Tiss Org Cult 56: 179-184
  3. Asthana R, Raina MK (1989) Pharmacology of Withania somnifera (Linn.) Dunal - a review. Ind Drugs 26: 199-205
  4. Baburaj S, Gunasekaran K (1995) In vitro differentiation of shoots from leaf callus cultures of Withania somnifera (L.) Dunal. J Ind Bot Soc 74: 323-324
  5. Canhoto JM, Lopes ML, Cruz GS (1999) Somatic embryogenesis and plant regeneration in myrtle (Myrtaceae). Plant Cell Tiss Org Cult 57: 13-21
  6. Choi YE, Kim HS, Soh WY, Yang DC (1997) Developmental and structural aspects of somatic embryos formed on medium containing 2,3,5-triiodobenzoic acid. Plant Cell Rep 16 738-744
  7. Jeya-Mary R, Jayabalan N (1997) Influence of growth regulators on somatic embryogenesis in sesame. Plant Cell Tiss Org Cult 49: 67-70
  8. Joseph T, Yeoh H-H, Loh C-S (2000) Somatic embryogenesis, plant regeneration and cyanogenesis in Manihot glaziovii Muell. Arg. (ceara rubber). Plant Cell Rep 19: 535-538
  9. Kebebew A, Gaj MD, Maluszynski M (1998) Somatic embryo-genesis and plant regeneration in callus cultures of tef, Eragrostis tef (Zucc.) Trotter. Plant Cell Rep 18: 154-15
  10. Kulkarni AA, Thengane SR, Krishnamurthy KV (1996) Direct in vitro regeneration of leaf explants of Withania somnifera (L.) Dunal. Plant Sci 119: 163-168
  11. Kulkarni AA, Thengane ST, Krishnamurthy KV (2000) Direct shoot regeneration from node, internode, hypocotyl and embryo explants of Withania somnifera. Plant Cell Tiss Org Cult 62:203-209
  12. Kulshreshtha S, Coutts RHA (1997) Direct somatic embryogenesis and plant regeneration from mature sugarbeet (Beta vulgaris L.) zygotic cotyledons. Plant Growth Regul 22: 87-92
  13. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473-497
  14. Rani G, Grover IS (1999) In vitro callus formation and regeneration studies in Withania somnifera. Plant Cell Tiss Org Cult 57: 23-27
  15. Rani G, Virk GS, Nagpal A (2003) Callus induction and plantlet regeneration in Withania somnifera (L.) Dunal. In Vitro Cell Dev Biol-Plant 39: (in press)
  16. Roja G, Heble MR, Sipahimalani AT (1991) Tissue cultures of Withania somnifera: morphogenesis and withanolide synthesis. Phytother Res 5: 185-187
  17. Sarasan V, Roberts AV, Rout GR (2001) Methyl laurate and 6-benzyladenine promote the germination of somatic embryos of hybrid rose. Plant Cell Rep 20: 183-186
  18. Sen J, Sharma AK (1991) Micropropagation of Withania somnifera from germinating seeds and shoot tips. Plant Cell Tiss Org Cult 26: 71-73
  19. Teli NP, Patil NM, Pathak HM, Bhalsing SR, Maheshwari VL (1999) Withania somnifera (Ashwagandha): regeneration through meristem culture. J Plant Biochem Biotechnol 8: 109-111
  20. Tetu T, Sangwan RS, Sangwan-Norreel BS (1987) Hormonal control of organogenesis and somatic embryogenesis in Beta vulgaris callus. J Exp Bot 38: 506-517
  21. Tiwari SK, Singh SP (1991) Micropropagation of Withania somnifera by tissue culture. Van Sand 15: 19-20
  22. Vesco LL dal, Guerra MP (2001) The effectiveness of nitrogen in Feijoa somatic embryogenesis. Plant Cell Tiss Org Cult 64: 19-25
  23. Wakhlu AK, Sharma RK (1998) Somatic embryogenesis and plant regeneration in Heracleum cadicans Wall. Plant Cell Rep 17:866-869
  24. Watin C, Bigot C (1989) Regeneration of fertile plants from embryogenic calli of common parsley (Petroselinum hortense Hoffm.). CR Sean Acad Sci Ser III 309: 653-659
  25. Wetzstein HY, Baker CM (1993) The relationship between somatic embryo morphology and conversion in peanut (Arachis hypogaea L). Plant Sci 92: 81-89