Clinical and Experimental Reproductive Medicine

  • 제31권4호
  • /
  • Pages.235-244
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  • 2004
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  • 2233-8233(pISSN)
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  • 2233-8241(eISSN)
대한생식의학회 (The Korean Society for Reproductive Medicine)
권호 표지

정자형성 과정에서 Vascular Endothelial Growth Factor 및 Endothelin-1 발현의 면역조직화학적 연구

The Influences of Vascular Endothlelial Growth Factor and Endothelin-1 on Speramtogenesis in Testis

  • 박성우 (부산대학교 의과대학 비뇨기과학교실) ;
  • 박현준 (부산대학교 의과대학 비뇨기과학교실) ;
  • 박남철 (부산대학교 의과대학 비뇨기과학교실)
  • Park, Sung-Woo (Department of Urology, Pusan National University Hospital) ;
  • Park, Hyun-Jun (Department of Urology, Pusan National University Hospital) ;
  • Park, Nam-Cheol (Department of Urology, Pusan National University Hospital)
  • 발행 : 2004.12.30
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초록

Objective: The effects on spermatogenesis by expression of vascular endothelial growth factor (VEGF) and endothelin-1 (ET-1) were investigated. Materials and Methods: Testicular specimens were obtained from 40 infertile males due to primary testicular failure and from 10 fertile males with other urologic problems. The specimens of infertile males were devided into 4 groups according to histologic findings; Sertoli cell only syndrome (A), maturation arrest (B), hypospermatogenesis (C) and sloughing and disorganization (D). VEGF and ET-1 expression were detected with immunohistochemical stain. Results: VEGF expression on Leydig cell was detected in all cases. But, VEGF expression rates on germ cell were significantly higher in infertile group B, C, D compared to that of the control group (p<0.05). ET-1 expression rates on Leydig cell was significantly lower in all infertile group compared to that of the control group (p<0.05). But, ET-1 expression rates on Sertoli cell was significantly higher in all infertile group compared to that of the control group (p>0.05). In germ cell of infertile group, LH, FSH and prolactin were significantly decreased, and estradiol is increased in positive stain group on ET-1 immunohistochemical stain (p<0.05). VEGF and ET-1 expression were not correlated mean seminiferous tubule diameter (p>0.05). Conclusions: Abnormal spermatogenesis would be reflected in VEGF expression in germ cell.

키워드

참고문헌

  1. Hargreave TB. Introduction. In: Hargreave TB, Soon TE, editors. The management of the male infertility.Singapore: PG Publishing 1990; 2-21
  2. Hedger MP, Meinhardt A. Cytokines and the imm-une-testicular axis. J Reprod Immunol 2003; 58: 1-26 https://doi.org/10.1016/S0165-0378(02)00060-8
  3. Holstein AF, Schulze W, Davidoff M. Understanding spermatogenesis is a prerequisite for treatment. Re-prod Biol Endocrinol 2003; 1: 107
  4. Huleihel M, Lunenfeld E. Regulation of spermatoge-nesis by paracrine/autocrine testicular factors. Asian J Androl 2004; 6: 259-68
  5. Ergϋn S, Kilic N, Harneit S, Paust HJ, Ungefroren H, Mukhopadhyay A, et al. Microcirculation and the va-scular control of the testis. Adv in Experim Med and Biol 1997; 424: 163-80 https://doi.org/10.1007/978-1-4615-5913-9_32
  6. Huminiecki L, Chan HY, Lui S Poulsom R, Stamp G, Harris AL, Bicknell R. Vascular endothelial growth factor transgenic mice exhibit reduced male fertility and placental rejection. Mol Hum Reprod 2001; 7: 255-64 https://doi.org/10.1093/molehr/7.3.255
  7. Korpelainen EI, Karkkainen MJ, Tenhunen A, La-kso M, Rauvala H, Vierula M, et al. Overexpression of VEGF in testis and epididymis causes infertility in transgenic mice: Evidence for nonendothelial tar-gets for VEGF. J Cell Biol 1998; 143: 1705-12 https://doi.org/10.1083/jcb.143.6.1705
  8. Maggi M, Barni T, Orlando C, Fantoni G, Finetti G, Vannelli GB et al. Endothelin-1 and its receptors in human testis. J Androl 1995; 16: 213-24
  9. Ergϋn S, Harneit, Paust HJ, Mukhopadhyay AK, Ho-lstein AF. Endothelin and endothelin receptor A and B in the human testis. Anat Embryol 1999; 199: 207-14 https://doi.org/10.1007/s004290050221
  10. Nalbandian A Dettin L, Dym M, Ravindranath N. Expression of vascular endothelial growth factor re-ceptors during male germ cell differentiation in the mouse. Biol of Reprod 2003; 69: 985-94 https://doi.org/10.1095/biolreprod.102.013581
  11. Senger DR, Connelly DT, Van De Water L, Feder J, Dvorak HF. Purification and NH2-terminal amino se-quence of guinea pig tumor secreted vascular perm-eability factor. Cancer Res 1990; 50: 1774-8
  12. Blanchard KT, Allard EK, Boekelheide K. Fate of germ cells in 2, 5-hexanedione-induced testicular in-jury. Toxicol Appl Pharmacol 1996; 136: 5-12
  13. Richburg JH, Boekelheide K. Mono- (2-ethyhexyl) phtalate rapidly alters both Sertoli cell vimentin fila-ments and germ cell apoptosis in young rat testes. Toxicol Appl Pharmacol 1996; 137: 42-50 https://doi.org/10.1006/taap.1996.0055
  14. Kim ED, Barqawi AZ, Seo JT, Meacham RB. Apo-ptosis: its importance in spermatogenic dysfunction. Urol Clin North Am 2002; 29: 755-65 https://doi.org/10.1016/S0094-0143(02)00093-9
  15. Zhang XW, Xu B. Differential regulation of p53, c-myc, Bcl-2, Bax and AFP protein expression, and caspase activity during 10-hydroxycamptochecin-induced apoptosis in Hep G2 cells. Anticancer Drugs 2000; 11: 747-56 https://doi.org/10.1097/00001813-200010000-00012
  16. 양주헌, 박현준, 박남철. 정자형성시 세포고사에 대한 c-kit 유전자의 면역조직화학적 연구. 대한비뇨회지 2003; 44: 1124-30
  17. Zachary I. Vascular endothelial growth factor. Int J Bio & Cell Biol 1998; 30: 1169-74 https://doi.org/10.1016/S1357-2725(98)00082-X
  18. Obermair A, Obruca A, Phl M, Kaider A, Vales A, Leodolter S, et al. Vascular endothelial growth factor and its receptor in male fertility. Fertil Steril 1999; 72: 269-75 https://doi.org/10.1016/S0015-0282(99)00234-4
  19. Yanagisawa M, Kurihara H, Kimura S, Tomobe Y, Kobayashi M, Mitsui Y et al. A novel vasoconstr-ictor poptide produced by vascular endothelial cells. Nature 1988; 332: 411-5 https://doi.org/10.1038/332411a0
  20. Ellahham SH, Charlon V, Abassi Z, Calis KA, Cho-ucair WK. Bosentan and the endothelin system in co-ngestive heart failure. Clin Cardiol 2000; 23: 803-7 https://doi.org/10.1002/clc.4960231128
  21. Fantoni G, Morris PL, Forti G, Vanelli GB, Orlando C, Barni T et al. Endothelin-1: A new autocrine/paracrine factor in rat testis. Am J Physiol 1993; 265: E267-74
  22. Matsumoto H, Suzuki N, Onda H, Fujino M. Abun-dance of endothelin-3 in rat intestine, pituitary gland and brain. Biochem Biophys Res Commun 1989; 164: 74-80 https://doi.org/10.1016/0006-291X(89)91684-7
  23. Sakaguchi H, Kozuka M, Hirose S, Ito T, Hagiwara H. Properties and localization of endothelin-1-specific receptors in rat testicles. Am J Physiol 1992; 263: R15-8
  24. Ohlstein EH, Douglas SA. Endothelin-1 modulates vascular smooth muscle structure and vasomotion: Implications in cardiovascular pathology. Drug De-velop Res 1993; 29: 108-28 https://doi.org/10.1002/ddr.430290207
  25. Takuwa Y. Endothelin in vascular and endocrine system: Biological activities and its mechanism of action. Endocr J 1993; 40: 489-506 https://doi.org/10.1507/endocrj.40.489
  26. Conte D, Morris PL, Forti G, Vanelli GB, Orlando C, Barni T et al. Endothelin stimulates testosterone secretion by rat Leydig cells. J Endocrinol 1993; 136: R1-4 https://doi.org/10.1677/joe.0.136R001
  27. Collin O, Damber JE, Bergh A. Effects of endothelin-1 on the rat testicular vasculature. J Androl 1996; 17: 360-6
  28. Tripiciano A, Peluso C, Morena AR, Palombi F, Ste-fanini M, Ziparo E et al. Cyclic expression of endo-thelin-converting enzyme-1 mediates the functional regulation of seminiferous tubule contraction. J Cell Biol 1999; 145: 1027-38 https://doi.org/10.1083/jcb.145.5.1027
  29. Rossi F, Ferraresi A, Romagni P, Silvestroni L, San-tiemma V. Angiotensin II stimulates contraction and growth of testicular peritubular myoid cells in vitro. Endocrinol 2002; 143: 3096-104 https://doi.org/10.1210/en.143.8.3096
  30. Fantoni G, Morris PL, Forti G, Vannelli GB, Orlando C, Barni T et al. Endothelin-1: A new autocrine/paracrine factor in rat testis. Am J Physiol 1993; 265: E267-74
  31. Romanelli F, Fillo S, Isidori A, Conte D. Stimulatory action of endothelin-1 on rat Leydig cells: Involve-ment of endothelin-A subtype receptor and phospho-lipase A2-arachidonate metabolism system. Life Sciences 1997; 61: 557-66 https://doi.org/10.1016/S0024-3205(97)00415-3
  32. Ergul A, Glassberg MK, Majercik MH, Puett D. Endothlin-1 promotes steroidogenesis and stimulates protooncogene expression in transformed murine Le-ydig cells. Endocrinology 1993; 132: 598-603 https://doi.org/10.1210/en.132.2.598