Clinical and Experimental Reproductive Medicine

  • 제31권4호
  • /
  • Pages.209-215
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  • 2004
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  • 2233-8233(pISSN)
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  • 2233-8241(eISSN)
대한생식의학회 (The Korean Society for Reproductive Medicine)
권호 표지

배아줄기세표의 인슐린 분비세포로의 유도 분화에 대한 연구

Induced Differentiation of Embryonic Stem Cells to Insulin Secreting Cells

  • 성지혜 (성균관대학교 의과대학 삼성제일병원 생식생물학 및 불임연구실) ;
  • 임천규 (성균관대학교 의과대학 삼성제일병원 생식생물학 및 불임연구실) ;
  • 최혜원 (성균관대학교 의과대학 삼성제일병원 생식생물학 및 불임연구실) ;
  • 이형송 (성균관대학교 의과대학 삼성제일병원 생식생물학 및 불임연구실) ;
  • 신현상 (성균관대학교 의과대학 삼성제일병원 생식생물학 및 불임연구실) ;
  • 전진현 (성균관대학교 의과대학 삼성제일병원 생식생물학 및 불임연구실) ;
  • 윤현수 (미즈메디병원 의과학연구소) ;
  • 궁미경 (성균관대학교 의과대학 삼성제일병원 산부인과)
  • Sung, Ji-Hye (Laboratory of Reproductive Biology & Infertility, Samsung Cheil Hospital, Sungkyunkwan University School of Medicine) ;
  • Lim, Chun-Kyu (Laboratory of Reproductive Biology & Infertility, Samsung Cheil Hospital, Sungkyunkwan University School of Medicine) ;
  • Choi, Hye-Won (Laboratory of Reproductive Biology & Infertility, Samsung Cheil Hospital, Sungkyunkwan University School of Medicine) ;
  • Lee, Hyoung-Song (Laboratory of Reproductive Biology & Infertility, Samsung Cheil Hospital, Sungkyunkwan University School of Medicine) ;
  • Shin, Hyeon-Sang (Laboratory of Reproductive Biology & Infertility, Samsung Cheil Hospital, Sungkyunkwan University School of Medicine) ;
  • Jun, Jin-Hyun (Laboratory of Reproductive Biology & Infertility, Samsung Cheil Hospital, Sungkyunkwan University School of Medicine) ;
  • Yoon, Hyun-Soo (Mizmedi Hospital, Medical Research Center) ;
  • Koong, Mi-Kyoung (Department of Ob/Gyn Samsung Cheil Hospital, Sungkyunkwan University School of Medicine)
  • 발행 : 2004.12.30
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초록

Objective: Embryonic stem (ES) cells could be differentiated into the specific cell types by alternation of culture condition and modification of gene expression. This study was performed to evaluate the differentiation protocol for mouse and human ES cells to insulin secreting cells. Methods: Undifferentiated mouse (JH-I) and human (Miz-hESI) ES cells were cultured on STO feeder layer, and embryoid bodies (EBs) were formed by suspension culture. For the differentiation, EBs were cultured by sequential system with three stage protocol. The differentiating ES cells were collected and marker gene expressions were analyzed by seIni-quantitative RT-PCR in each stage. Amount of secreted insulin levels in culture media of human ES cells were measured by human insulin specific RIA kit. Results: During the differentiation process of human ES cells, GATA-4, a-fetoprotein, glucose transporter-2 and Ngn-3 expression were increased whereas OctA was decreased progressively. Insulin and albuInin mRNAs were expressed from stage IT in mouse ES cells and from stage III in human ES cells. We detected 3.0~7.9 IlU/rnl secretion of insulin from differentiated human ES cells by in vitro culture for 36 days. Conclusion: The sequential culture system could induce the differentiation of mouse and human ES cells into insulin secreting cells. This is the fIrst report of differentiation of human ES cells into insulin secreting cells by in vitro culture with serum and insulin free medium.

키워드

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