Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Development of Doubled-haploid Population and Construction of Genetic Map Using SSR Markers in Rice

벼의 Doubled-haploid 집단육성과 SSR 마커를 이용한 유전자 지도작성

  • 김경민 (경북대학교 유전공학연구소) ;
  • 남우일 (경북대학교 식물생명과학부) ;
  • 권용삼 (국립종자관리소 재배시험과) ;
  • 손재근 (경북대학교 식물생명과학부)
  • Published : 2004.09.01
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Abstract

A doubled-haploid (DH) population was developed through anther culture of F$_1$ plants obtained from a cross between a japonica cultivar, 'Nagdongbyeo', as male parent and a indica cultivar, 'Samgangbyeo', as female parent. Segregation modes for plant length, culm length, panicle length, third internode length, and days to heading in the DH lines showed nearly normal distribution with wide range of variation. A molecular map with 136 simple sequence repeat (SSR) markers was constructed using the DH population. The total map distance was 1,909 cM and the average interval of marker distance was 14 cM.

본 연구에서는 1998년부터 2003년 하계까지 약배양 기법 및 콜히친 처리를 이용하여 개발한 '삼강벼/낙동벼' DH(doubled-haploid) 183계통의 주요 농업 형질을 조사$.$분석하였다. DH 집단의 주요 농업형질을 조사한바 초장, 간장, 수장, 삼절간장, 수수 및 출수일수는 양적형질의 특징인 넓은 범위의 변이폭, 연속적인 빈도 분포양상 및 양친을 초월하는 초월분리 현상을 보였다. SSR 마커를 이용한 유전자지도의 작성에는 양친에 다형성을 나타내는 136개의 마커를 사용하였다. 작성된 유전자 지도는 전체 길이가 1,909cM이었으며, 마커간 평균길이는 14 cM을 나타내었다.

Keywords

References

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