한국양식학회지 (Journal of Aquaculture)

한국양식학회 (The Korean Society of Fisheries and Aquatic Science)
권호 표지

한국산 백합과 5종의 유전적 유연관계

Genetic Relationship of the Five Venerid Clams유 (Bivalvia, Veneridae) in Korea

  • 정형택 (여수대학교 수산생명과학부) ;
  • 김정 (수산증양식 연구센터) ;
  • 신종암 (여수대학교 수산생명과학부) ;
  • 서호영 (여수대학교 수산생명과학부) ;
  • 최상덕 (여수대학교 수산생명과학부)
  • 발행 : 2004.11.01
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초록

상업적 가치가 높고 양식 가능성이 있는 백합과 5종을 대상으로 RAPD방법을 이용한 개체간의 유전적 유연관계를 조사하였다. DNA 추출은 protein K-phenol방법을 사용하여 각 종의 후폐각근에서 추출하였다. RAPD-PCR결과 15개의 primer들이 증폭되었고, 그들로부터 각각 1-3개의 band를 볼 수 있었다. 백합과 5종간 유전적 유사도는 살조개와 바지락간에는 0.84, 개조개와 백합간에는 0.87로 각각의 두 종간에는 놓은 유사도를 보였고, 가무락조개는 살조개와 바지락간에 0.78의 유사도를 보였으며, 개조개와 백합, 살조개, 바지락, 가무락조개간에는 0.46의 비교적 낮은 유전적 유사도를 보였다. RAPD 방법으로 종내개체 유전변이를 파악하기는 힘들어도, 양식이나 자원증식을 위한 패류의 우수형질 선택에 있어 기본 정보를 제공 할 수는 있을 것이다. 또한, 살조개 대량종묘생산의 방법은 유전적 유사도가 가장 가까운 바지락을 중심으로 이루어져야 할 것으로 사료된다.

The random amplified polymorphic DNAs (RAPD) technique was used to characterize the genetic relationship of five species from in the family of Veneridae which is one of the commercially important clam family in Korea. The veneride clams' DNA were extracted from adductor muscular by the proteinase K-phenol method. Among 20 primers, 15 unit primers were amplified and produced at least, 2 or 3 from the top band. Genetic similarity between the purplish washington, Saxidomus purpuratus and the hard clam, Meretrix lusoria was the highest (0.87); the lowest genetic similarity (0.46) was formed between the little clam, Ruditapes philppinarum and the purplish washington, S. purpuratus. The genetic relationship between the venus clam, Protothaca jedoensis and the little clam, R. philppinarum was a closer than those between others. These results may indicate that the method of artificial seeding production of P. jedoensis for the propagation of resources can be focused on R. philppinarum.

키워드

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