Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
권호 표지

Combined Effects of Temperature and Salinity on Survival and Hemolymph Osmoregulation of Litopenaeus vannamei

흰다리새우 Litopenaeus vannamei의 생존과 혈장 삼투질 조절에 미치는 수온과 염분의 영향

  • 김대현 (국립수산과학원 서해수산연구소 갑각류연구센터) ;
  • 김봉래 (국립수산과학원 서해수산연구소 갑각류연구센터) ;
  • 김종식 (국립수산과학원 서해수산연구소 갑각류연구센터) ;
  • 서형철 (국립수산과학원 서해수산연구소 갑각류연구센터) ;
  • 김수경 (국립수산과학원 서해수산연구소 갑각류연구센터) ;
  • 김종화 (국립수산과학원 서해수산연구소 갑각류연구센터) ;
  • 장인권 (국립수산과학원 서해수산연구소 양식연구팀)
  • Published : 2004.11.01
Download PDF
⟨ Previous Next ⟩

Abstract

Survival of 12.7 g (average body weight) Litopenaeus vannamei was examined at temperatures of 14, 16, 18, 28, 32, 34 and 36$^{\circ}C$ and salinities of l0, 20, 30 and 40 ppt after 7 days. Hemolymph osmolality was measured at 12 combinations of salinities (l0, 20, 30 and 40 ppt) and temperature levels (18, 28 and 34$^{\circ}C$) after 14 days. The results show that the species have their best survival between temperatures of 18 and 3$0^{\circ}C$ and salinity above 20 ppt. Hemolymph osmolality increased with increased salinity at all temperatures tested. The isosmotic point calculated from the linear relationship between hemolymph osmolality and medium osmolality and recorded as 826, 809 and 1,117 mOsm/kg which is equivalent to 29.4, 28.8 and 40.2 ppt at 18, 28 and 34$^{\circ}C$, respectively. The slopes obtained from the relationship between hemolymph osmolality and medium osmolality were 0.314, 0.276 and 0.541 for L. vannamei at 18, 28 and 34$^{\circ}C$, respectively, suggesting that the shrimp at 34$^{\circ}C$ regulated osmotic concentration much worse than those at 18 and 28$^{\circ}C$. This result also indicated that L. vannamei living at 34$^{\circ}C$, showed more fluctuation in hemolymph osmolality than those at 18 and 28$^{\circ}C$.

Keywords

References

  1. Bishop, J. M., J. G. Gosselink and J. H. Stone, 1980. Oxygen consumption and hemolymph osmolality of brown shrimp, Penaeus aztecus. Fish. Bull., 78: 741-757
  2. Bray, W. A., A. L. Lawrence and J. R. Leung-Trujillo, 1994. The effect of high salinity on the survival and growth of juvenile Penaeus vannamei, P. styliostris, and P. nwnodon. in: Abstracts. World Aquaculture, 90, 121/CP6. National Research Council, Ottawa, Ontario, Canada
  3. Castille, F. L. Jr and A. L. Lawrence, 1981. The effect of salinity on the osmotic, sodium, and chloride concentrations in the hemolymph of euryhaline shrimp of the genus Penaeus. Compo Biochem. Physiol., 68: 75-80 https://doi.org/10.1016/0300-9629(81)90320-0
  4. Cawthorne, D. E., T. W. Beard, J. Davenport and J. E Wickins, 1983. Response of juvenile Penaeus monodon Fabricius to natural and artificial seawaters of low salinity. Aquaculture, 32: 165-174 https://doi.org/10.1016/0044-8486(83)90278-8
  5. Charmantier, G., 1975. Variations saisonnieres des capacites ionoregulatrices de SphaerOina serratum (Fabricius, 1787) (Crustacea, lsopda, Flabellifera). Comp. Biochem. Physiol., 68: 7580
  6. Charmantier, G., C. Soyez and C. Aquacop, 1994. Effect of molt stage and hypoxia on osmoregulatory capacity of the penaeid shrimp Penaeus vannamei. J. Exp. Mar. BioI. Ecol., 178: 233-246 https://doi.org/10.1016/0022-0981(94)90038-8
  7. Charmantier-Daures, M., P. Thuet, G. Charmantier and J. P. Trilles, 1988. Tolerance a la salinite et osmoregulation chez les post-larves de Penaeus japonicus et P. chinensis Effect de la temperature. Aquat. Living Resource, 1: 267-276 https://doi.org/10.1051/alr:1988026
  8. Chen, J. C. and J. N. Lin, 1994. Osmolality and chloride concentration in the hemolymph of subadult Penaeus chinensis subjected to different salinty levels. Aquaculture, 125: 167-174 https://doi.org/10.1016/0044-8486(94)90293-3
  9. Chen, J. C. and J. N. Lin, 1998. Osmotic concentration and tissue water of Penaeus chinensis juveniles reared at different salinity and temperature levels. Aquaculture, 164: 173-181 https://doi.org/10.1016/S0044-8486(98)00185-9
  10. Chen,J. C., M. N. Lin, Y. Y. Ting and J. N. Lin, 1995. Survival, haemolymph osmolality and tissue water of Penaeus chinensis juveniles acclimated to different salinity and temperature levels. Compo Biochem. Physiol., 110: 253-258 https://doi.org/10.1016/0300-9629(94)00164-O
  11. Dehnel, P. A., 1962. Aspects of osmoregulation in the crab Hemigrapsus nudus. Can. J. Zool., 52: 923-937 https://doi.org/10.1139/z74-123
  12. Ferraris, R. P., F. D. Parado-Estepa, J. M. Ladja and E. G. De Jesus, 1986. Effect of salinity on the osmotic, chloride, total protein and calcium concentrations in the hemolymph of prawn Penaeus monodon (Fabricius). Compo Biochem. Physiol., 83: 701-708 https://doi.org/10.1016/0300-9629(86)90713-9
  13. Han, C. H., 1988. Physiological studies on the reproductive cycle of a freshwater prawn, Macrobrachium nipponense (De Haan). PhD Thesis, Faculty of Agriculture, University of Tokyo, 1988
  14. Lignot, J. H, J. C. Cochard, C, Soyez, P. Lemaire and G. Charmantier, 1999. Osmoregulatory capacity according to nutritional status, molt stage and body weight in Penaeus stylirostris. Aquaculture, 170: 79-92 https://doi.org/10.1016/S0044-8486(98)00392-5
  15. Lin, H. P., P. Thuet, J. P. Trilles and R. Mounet-Guillaume, 1993. Effect of ammonia on survival and osmoregulation of various development stages of shrimp Penaeus japonicus. Mar. BioI., 117: 591-598 https://doi.org/10.1007/BF00349770
  16. Lotz, J. M. and J. T. Ogle. 1994. Reproductive performance of the white-legged shrimp Penaeus vannamei in recirculating seawater systerrns. J. Word Aqua. Soc., 22: 167-172
  17. Parado-Estepa, F. D., R. P. Ferraris, J. M. Ladja and F. G. De Jesus, 1987. Response of intermolt Penaeus indicus to large fluctuations in environmental salinity. Aquaculture, 64: 175184 https://doi.org/10.1016/0044-8486(87)90323-1
  18. Ponce-Palafox, C., A Martinez-Palacios and L. G. Ross. 1997. The effects of salinity and temperature on the growth and survival rates of juvenile white shrimp, Penaeus vannamei, Boone, 1931. Aquaculture, 157: 107-115 https://doi.org/10.1016/S0044-8486(97)00148-8
  19. Shiau, S. Y., 1998. Nutrient requirements of penaeid shrimps. Aquaculture, 164: 77-93 https://doi.org/10.1016/S0044-8486(98)00178-1
  20. Williams, A. B., 1960. The influence of temperature on osmotic regulation in two species of estuarine shrimps (Penaus). BioI. Bull., (Woods Hole, Mass) 119: 560-571 https://doi.org/10.2307/1539268
  21. 구자근, 1999. 대하, Penaeus chinensis 양식장의 환경 특성과 생산성에 관한 연구. 부경대학교 대학원 석사학위논문, 57 pp