대한한의학회지 (The Journal of Korean Medicine)

  • 제25권2호
  • /
  • Pages.67-76
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  • 2004
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  • 1010-0695(pISSN)
  • /
  • 2288-3339(eISSN)
대한한의학회 (The Society of Korean Medicine)
권호 표지

팔물탕이 저산소증에 의한 배양심근세포고사에 미치는 영향

Protective Effects of Palmul-tang on Hypoxia-induced Apoptosis in H9c2 Cardiomyoblast Cells

  • 임은경 (원광대학교 전주한방병원 심계내과학교실) ;
  • 신선호 (원광대학교 전주한방병원 심계내과학교실)
  • 발행 : 2004.06.01
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초록

Objectives : This study was designed to investigate the protective mechanisms of Palmul-tang on hypoxia-induced cytotoxicity in H9c2 cardiomyoblast cells. Methods : In this study, we used H9c2 cells. Cells were subjected to hypoxia in the absence and presence of $1000\mu\textrm{g}/ml$ Palmul-tang for 24 hrs. Cells were treated with various concentrations of Palmul-tang for 24 hrs. Cell viability was measured by MTT assay. Hypoxia markedly decreased the viability of H9c2 cells, which was characterized with apparent apoptotic features such as chromatin condensation as well as fragmentation of genomic DNA and nuclei. Results : Palmul-tang significantly reduced hypoxia-induced cell death and apoptotic characteristics. Also, Palmul-tang prevented mitochondrial dysfunction including the disruption of mitochondrial membrane permeability transition (MPT) and an increase in expression of apoptogenic Bcl-2 proteins in hypoxia-H9c2 cells. Conclusions; This study suggests that the protective effects of Palmul-tang against hypoxic damages may be mediated by the modulation of Bcl-2, Bax expression.

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