The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
권호 표지

Protective Effects of Palmul-tang on Hypoxia-induced Apoptosis in H9c2 Cardiomyoblast Cells

팔물탕이 저산소증에 의한 배양심근세포고사에 미치는 영향

  • 임은경 (원광대학교 전주한방병원 심계내과학교실) ;
  • 신선호 (원광대학교 전주한방병원 심계내과학교실)
  • Published : 2004.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : This study was designed to investigate the protective mechanisms of Palmul-tang on hypoxia-induced cytotoxicity in H9c2 cardiomyoblast cells. Methods : In this study, we used H9c2 cells. Cells were subjected to hypoxia in the absence and presence of $1000\mu\textrm{g}/ml$ Palmul-tang for 24 hrs. Cells were treated with various concentrations of Palmul-tang for 24 hrs. Cell viability was measured by MTT assay. Hypoxia markedly decreased the viability of H9c2 cells, which was characterized with apparent apoptotic features such as chromatin condensation as well as fragmentation of genomic DNA and nuclei. Results : Palmul-tang significantly reduced hypoxia-induced cell death and apoptotic characteristics. Also, Palmul-tang prevented mitochondrial dysfunction including the disruption of mitochondrial membrane permeability transition (MPT) and an increase in expression of apoptogenic Bcl-2 proteins in hypoxia-H9c2 cells. Conclusions; This study suggests that the protective effects of Palmul-tang against hypoxic damages may be mediated by the modulation of Bcl-2, Bax expression.

Keywords

References

  1. 瑞竹堂經驗方: 欽定四庫全書(卷十四) 沙圖穆蘇
  2. 醫方集解 王昻
  3. J.Biol.Chem. v.263 Measurement of superoxide-derived free radicals in the reperfused heart. Evidence for a free radical mechanism of reperfusion injury Zweier JL
  4. Mol Cell Biochem. v.147 Oxidative stress and heart failure Singh N;Dhalla AK;Seneviratne C;Singal PK
  5. Pharmacol Res. v.43 no.2 Protective effects of flavonoids in the roots of Scutellaria baicalensis Georgi against hydrogen peroxide-induced oxidative stress in SH-SY5Y cells Gao Z;Huang K;Xu H
  6. 기아회복시 대사기질, 호르몬, 혈액성분변화에 미치는 四君子湯, 四物湯 및 八物湯의 효과 姜政秀
  7. 八物湯 및 B.E.P.가 運動負荷로 惹起된 筋肉疲勞와 肝代謝酵素에 미치는 實驗的 硏究 吳旼錫
  8. 四君子湯, 四物湯 및 八物湯이 근육피로회복에 미치는 실험적 연구 李哲浣
  9. 少陰人 八物君子湯과 升陽益氣湯이 Hydrocortisone Acetate로 誘發된 陽虛證에 미치는 實驗的 硏究 金鎭成
  10. 동의병리학회지 v.10 八物湯이 抗癌 및 免疫調節作用에 미치는 影響 河智容(외)
  11. 八物湯加味方이 마우스의 貪食細胞機能 및 免疫反應에 미치는 影響 田炳薰
  12. 배양심근세포의 산화적 손상에 대한 四君子湯의 방어효과 鄭雨哲
  13. 배양심근세포의 산화적 손상에 대한 四物湯의 방어효과 趙權一
  14. 四君子湯, 二陳湯, 六君子湯이 高脂血症에 미치는 影響 李承宰
  15. 四物湯 및 四君子湯이 endotoxin으로 유발된 血栓症에 미치는 影響 河智容
  16. 四物湯이 血管內皮細胞에 미치는 影響 南昌圭
  17. 東醫寶鑑 許浚
  18. 校註婦人良方: 欽定四庫全書(卷三十一, 薛氏醫案) 薛己
  19. 醫學入門 이천
  20. 方藥指針講義抄錄(下) 孟華燮
  21. 原色臨床本草學 辛民敎
  22. 完譯中藥大辭典 김창민(외)
  23. 生脈散이 實驗動物의 心筋細胞에 미치는 影響 申善澔
  24. 丹參飮 煎湯液이 培養 心筋細胞에 미치는 影響 孫昶翼
  25. 심근세포의 산화적 손상에 대한 五味子湯의 효과 및 작용기전 연구 黃普淵
  26. 通靈散 抽出物이 培養 心筋細胞에 미치는 影響 成恩慶
  27. Cir Res. v.81 Death by design. Programmed cell death in cardiovascular biology and disease MacLellan WR;Schneider MD
  28. Toxicol Appl Pharmacol. v.159 no.3 Metallothionein and apoptosis during differentiation of myoblasts to myotubes: protection against free radical toxicity Apostolova MD;Ivanova IA;Cherian MG
  29. Arch Biochem Biophys. v.373 no.1 Hydrogen peroxide dose dependent induction of cell death or hypertrophy in cardiomyocytes Chen QM;Tu VC;Wu Y;Bahl JJ
  30. Science v.262 Programed cell death and the control of cell survival Raff MC;Bares BA;Burne JF;Coles HS;Ishizaki Y;Jacobson MD
  31. Circ Res. v.78 no.4 Mechanism of hydrogen peroxide and hydroxyl free radical-induced intracellular acidification in cultured rat cardiac myoblasts Wu ML;Tsai KL;Wang SM;Wu JC;Wang BS;Lee YT
  32. Cancer Res.1 v.63 no.19 Predominant Role of Hypoxia-Inducible Transcription Factor (Hif)-lalpha versus Hif-2alpha in Regulation of the Transcriptional Response to Hypoxia Sowter HM;Raval R;Moore J;Ratcliffe PJ;Harris AL
  33. J Appl Physiol. v.95 no.5 Effect of hyperbaric oxygen on apoptosis in neonatal hypoxia-ischemia rat model Calvert JW;Zhou C;Nanda A;Zhang JH
  34. Biochem. Biophys. Res. Comm. v.224 Constituvely enhanced nbl expression is associated with the induction of inter-nucleosomal DNA cleavage by actinomycin D Naora, H.;Nishida, T.;Shindo, Y.;Adachi, M.;Naora, H.
  35. Cardiovasc Toxicol. v.3 HSP60, Bax, and Cardiac Apoptosis Knowlton AA;Gupta S
  36. CMLS v.54 Mechanism controlling cellular suicide: role of Bcl-2 and caspases Allen RT;Cluck MW;Agrawal DK
  37. Nature Med. v.6 The mitochondrial death/life regulator in apoptosis and necrosis Kroemer G;Dallaporta B;Resche-rigon M
  38. Science v.245 Monoclonal antibody-mediated tumor regression by induction of apoptosis Trauth BC;Klas C;Peters A. MJ;Matzuku S;Moller P;Falk W;Debatin KM; Krammer PH
  39. EMBO v.16 Activation of CD95 (APO-1/Fas) signaling by ceramide mediates cancer therapy-induced apoptosis Herr, I.;Wilhelm, D.;Bohler, T.;Angel, P.;Debatin, K. M.
  40. Circ Res. v.85 no.12 ARC inhibits cytochrome c release from mitochondria and protects against hypoxia-induced apoptosis in heart-derived H9c2 cells Ekhterae D;Lin Z;Lundberg MS;Crow MT;Brosius FC 3rd;Nunez G