Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Biosynthesis of Cephalexin in PEG400-Ammonium Sulfate and PEG400-Magnesium Sulfate Aqueous Two-Phase Systems

  • Cao, Xuejun (State Key Laboratory of Bioreactor Engineering, Department of Biochemical Engineering, East China University of Science and Technology) ;
  • Zhu, Jianhang (State Key Laboratory of Bioreactor Engineering, Department of Biochemical Engineering, East China University of Science and Technology) ;
  • Wei, Dongzhi (State Key Laboratory of Bioreactor Engineering, Department of Biochemical Engineering, East China University of Science and Technology) ;
  • Hur, Byung-Ki (Department of Biological Engineering, Inha University)
  • Published : 2004.02.01
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Abstract

The biosynthesis of cephalexin was carried out in the aqueous two-phase systems using penicillin acylase as a catalyst, and 7-aminodeacetoxicephalosporanic acid (7-ADCA) and phenylglycine methyl ester (PGME) as substrates. 20% PEG400-l7.5% ${(NH_4)}_2SO_4$ containing 0.5 M NaCl and 1.5 M methanol aqueous two-phase systems (ATPS) were selected as reaction medium, and 53% product yield was obtained using immobilized penicillin acylase as a catalyst. 20% PEG400-l5% $MgSO_4$ ATPS was also used for the synthesis of cephalexin, and 60-62% product yield was obtained by using free penicillin acylase as a catalyst. When batch reactions were repeated in the ATPS, the cephalexin yields decreased during the reactions due to deactivation, loss, and product inhibition of penicillin acylase. The effect of different ratio of phenylglycine methyl ester to 7-ADCA on the product yield was investigated, and high cephalexin yield was obtained at a high molar ratio.

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