Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Effect of Radiation on mRNA Expression of Ceruloplasmin Gene

방사선 조사에 따른 U-937 세포의 Ceruloplasmin 유전자에서 mRNA 발현 변화

  • 오연경 (경기대학교 생물학과) ;
  • 임희영 (경기대학교 생물학과) ;
  • 김종수 (경기대학교 생물학과) ;
  • 윤충효 (농업생명공학연구원 기초기술개발과) ;
  • 김인규 (한국원자력연구소 방사선생체해석분야) ;
  • 윤병수 (경기대학교 생물학과)
  • Published : 2004.03.01
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Abstract

Against environmental stress, ceruloplasmin which is a plasma protein, are believed to play central roles in antioxidant- or peroxidase-activity in blood stream to remove free radicals, which may be caused by exposing of $\gamma$-irradiation. In human U-937 cells exposed to $\gamma$-irradiation, the levels of mRNA in ceruloplasmin gene were measured on 0, 4, 12, 24 hr after exposing by using comparative RT-PCR (Reverse transcriptase-polymerase chain reaction) which was achieved to compare with house keeping genes such as $\beta$-actin and hprt. After $\gamma$-irradiation of 100 rads or 200 rads, the total quantities of RNA were increased as dose and time dependent manner. On the contrary, the variation of mRNA expression in ceruloplasmin was not found until 4 hr after irradiation. After 12 hr and 24 hr of irradiation, the levels of mRNA in ceruloplasmin were significantly increased as dose and time dependent manner than un-exposed cells.

Keywords

References

  1. Harris, E.D. (1991): Copper transport: an over view. Proc. Soc. Exp. Biol. Med., 196, 130-140
  2. Ikawa, M. and Shozen, Y. (1990): Quantification of acute phase proteins in rat serum and in the supernatants of a cultured rat hepatoma cell line and cultured primary hepa-tocytes by an enzyme-linked immunosorbent assay. J. lmmunol. Methods, 134, 101-106 https://doi.org/10.1016/0022-1759(90)90117-E
  3. Kim, I.G. and Park, S.Y. (1998): Requirement of intact human ceruloplasmin for the glutathione-linked peroxidase activity. FEBS Lett., 437, 293-296
  4. Kim, I.G., Park, S.Y. Kim, K.C. and Yum, J.J. (1998): Thiol-linked peroxidase activity of ceruloplsmin. FEBS Lett., 431, 473-475
  5. Magic, Z., Matic-Ivanic, S., Savic, J. and Poznanovic, G. (1995): Ionizing radiation-induced expression of the genes associationed with the acute response to injuri in the rat. Radial. Res., 143, 187-193
  6. Oh, Y.K., Park, S.H., Kim, I.G. and Yoon, B.S. (2002): Cerulo-plasmin gene expression in U-937 cells exposed to gamma-irradiation and H20 2. Enviromental Mutagens & Carcinogens, 22, 76-82
  7. Onizuka, K, Migita, S., Yamada, H. and Matsumoto, I. (1999): Serum protein tractions in patients with laryngeal cancer undergoing radiation therapy. Possibility as prognostic tractor. Fukuoka Igaku Zasshi. 90, 46-58
  8. Sato and Gitlin, J.D. (1991): Mechanisms of copper incorporation during the biosynthesis of human ceruloplasmin. J. Bioi. Chem., 266, 5128-5134
  9. Siano, Y.S., Wakusawa, H., Hayashi, H., Takikawa, T., Yano, M., Okada, T., Abuchi, H., Kono, S. and Miyajima, H. (2001): Iron accumulation in the liver of male patients with Wilson's disease. Am. J. Gastroenterol., 96, 3417-3151 https://doi.org/10.1111/j.1572-0241.2001.05271.x
  10. Yukitoshi, Y., Tsugutoshi, A., Shinichirou, A., Toshihiro, O., Gorou, T., Teruo, K., Yousuke, S., Morimi, S., Masanori, K., Michinori, I. and Fumio. E. (1999): Mass screening 10r Wilson's disease: Results and recommendations. Pediatrics International, 41, 405-408 https://doi.org/10.1046/j.1442-200x.1999.01096.x
  11. Zong, M.Q. and Ye, K. (2001): Rethinking the role of cerulo-plasmin in brain iron metabolism. Brain Research Review, 35, 287-294