한국임상수의학회지 (Journal of Veterinary Clinics)

  • 제21권3호
  • /
  • Pages.286-290
  • /
  • 2004
  • /
  • 1598-298X(pISSN)
  • /
  • 2384-0749(eISSN)
한국임상수의학회 (The Korean Society of Veterinary Clinics)
권호 표지

림프구의 미소핵을 지표로 영광 원자력발전소 주변 사육 돼지의 방사선 생물학적 평가

Radiobiological Evaluation in Pig Bred in the Vicinity of Yeonggwang Nuclear Power Station Using Micronuclei in Cytokinesis-blocked Lymphocyte

  • 발행 : 2004.09.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Cytogenetic and hematological analysis was performed in peripheral blood of pig in the vicinity of Yeonggwang nuclear power station and control area. The frequency of micronuclei (MN) in peripheral blood lymphocytes from pig was used as a biomarker of radiobiological effects resulting from exposure to environmental radiation. An estimated dose of radiation was calculated by a best fitting linear-quadratic model based on the radiation-induced MN formation from the swine lymphocytes exposed in vitro to radiation over the range from 0 Gy to 4 Gy. MN rates in lymphocytes of pig from Yeonggwang nuclear power station and control area were 10.60/1,000 and 11.10/1,000, respectively. There were no significant differences in MN frequencies and hematological values in pig between Yeonggwang and control area. The study indicates that the MN assay in lymphocyte of pig is a rapid, sensitive and accurate method that can be used to monitor a large population exposed to radiation.

키워드

참고문헌

  1. Almassy Z, Krepinsky AB, Bianci A, Koteles GJ. The present state and perspectives of micronucleus assay in radiation protection. A review. Appl Radiat Isot 1987; 38: 241-249 https://doi.org/10.1016/0883-2889(87)90033-5
  2. Backer LC, Grindem CB, Corbett WT, Cullins L, Hunter JL. Pet dogs as sentinels for environmental contamination. Sci Total Environ 2001; 274: 161-169 https://doi.org/10.1016/S0048-9697(01)00740-9
  3. Brewen JG, Preston RJ, Jones KP, Gosslee DG. Genetic hazards of ionizing radiations: cytogenetic extrapolations from mouse to man. Mutat Res 1973; 17: 245-254 https://doi.org/10.1016/0027-5107(73)90173-5
  4. Brewen JG, Preston RJ, Littlefield LG. Radiation-induced human chromosome aberration yields following an accidental whole-body exposure to $^{60}Co$ gamma-rays. Radiat Res 1972; 49: 647-656 https://doi.org/10.2307/3573421
  5. Catena C, Asprea L, Carta S, Tortora G, Conti D, Parasacchi P, Righi E. Dose-response of X-irradiated human and equine lymphocytes. Mutat Res 1997; 373: 9-16 https://doi.org/10.1016/S0027-5107(96)00178-9
  6. Chung HW, Kim SY, Sohn EH, Ha SW. Analysis of chromosome aberrations in nuclear-power-plant workers considering the lifetime of lymphocytes. Int J Radiat BioI 2000; 76: 923-927 https://doi.org/10.1080/09553000050050936
  7. Fieldner TM, Nothdurft W, Steinbach KH. Blood cell changes after radiation exposure as an indicator for hemopoietic stem cell function. Bone Marrow Trans 1988; 3: 77-84
  8. Flores MJ, Pinero J, Ortiz T, Pastor N, Mateos JC, Cortes F. Both bovine and rabbit lymphocytes conditioned with hydrogen peroxide show an adaptive response to radiation damage. Mutat Res 1996; 372: 9-15 https://doi.org/10.1016/S0027-5107(96)00082-6
  9. Griffin CS, Scott D, Papworth DG. The influence of DNA content and nuclear volume on the frequency of radiation-induced chromosome aberrations in Bufo species. Chromosoma 1970; 30: 228-230
  10. He JL, Jin HY, Jin LF, Gao SY. Monitoring of human exposure to radiation with the binucleated lymphocyte micronucleus assay. Biomed Environ Sci 2000; 13: 32-36
  11. lAEA. Biological dosimetry : chromosomal aberration analysis for dose assessment, Technical report 260. Vienna: lAEA publications. 1986
  12. IAEA. Biological dosimetry with particular reference to chromosome aberration analysis. A review of methods. Vienna:IAEA-SM-199/4, Vienna: IAEA publications. 1969
  13. Ishihara T, Sasaki M. Radiation-induced chromosome damage in man. New York: Alan R. Liss. 1983: 561-583
  14. Kim SH, Han DU, Lim JT, Jo SK, Kim TH. Induction of micronuclei in human, goat, rabbit peripheral blood lymphocytes and mouse splenic lymphocytes irradiated in vitro with gamma radiation. Mutat Res 1997; 393: 207-214 https://doi.org/10.1016/S1383-5718(97)00103-4
  15. Leonard A, Gerber GB, Papworth DG, Decat G, Leonard ED, Deknudt G. The radiosensitivities of lymphocytes from pig, sheep, goat and cow. Mutat Res 1976; 36: 319-332 https://doi.org/10.1016/0027-5107(76)90242-6
  16. Lloyd DC. An overview of radiation dosimetry by conventional cytogenetic method. Berlin, Heidleberg, New York, Tokyo: Sprinr-Verlag. 1984: 3-13
  17. Ludewig E, Koch F, Kamprad F, Melzer R. The micronucleus test in pigs: induction of micronuclei in polychromatic erythrocytes by various doses of X-rays. Mutat Res 1991; 249: 1-6 https://doi.org/10.1016/0027-5107(91)90127-A
  18. Muirhead CR, Boice JDJr, Raddatz CT, Yoder RC. Comparison of dose histories for U.S. nuclear power plant workers, based on records held by a major dosimetry service company and on the NRC REIRS database. Health Phys 1996; 70: 645-650 https://doi.org/10.1097/00004032-199605000-00004
  19. Muller W-U, Streffer C. Biological indicators for radiation damage. Int J Radiat Biol 1991; 59: 863-873 https://doi.org/10.1080/09553009114550771
  20. Peace BE, Succop P. Spontaneous micronucleus frequency and age: what are normal values? Mutat Res 1999; 425: 225-230 https://doi.org/10.1016/S0027-5107(99)00030-5
  21. Ramalho A, Sunjevaric I, Natarajan AT. Use of frequencies of micronuclei as quantitative indicators of X-ray-induced chromosome aberrations in human peripheral blood lymphocytes: comparison of two methods. Mutat Res 1988; 207: 141-146 https://doi.org/10.1016/0165-7992(88)90078-4
  22. Ramalho AJ, Nascimento ACH, Natorajan AT. Dose assesments by cytogenetic analysis in the Goiania(Brasil) radiation accident. Radiat Protect Dosimetry 1988; 25: 97-100
  23. Scarfi MR, Lioi MB, Di Berardino D, Zeni O, Coviello AM, Matassino D. Measurement of micronuclei by cytokinesisblock method in bovine lymphocytes. Mutat Res 1993; 289: 291-295 https://doi.org/10.1016/0027-5107(93)90080-Y
  24. Shliakhtenok AS. Dynamics of 134 + 137Cs accumulation in insects inhabiting the 30-kilometer zone of Chernobyl Nuclear Power Station. Radiats Biol Radioecol 2003; 43: 93-96
  25. Spirin EV. Reconstruction of I-131 in milk and exposure doses to the thyroid gland of cattle after the Chernobyl AES. Radiats Biol Radioecol 2002; 42: 564-568
  26. Stenphan G, Hadnagy W, Hammermaier C, Imhof U. Biologically and physiologically recorded doses after an accidental exposure to $^{60}Co$ gamma-rays. Health Phys 1983; 44: 409-411
  27. Straube E, Straube W, Romer T. Does occupational nuclear power plant radiation affect conception and pregnancy? Early Pregnancy 1995; 1: 130-133
  28. Sutiakova I, Sulik E, Rimkova S, Sakalikova A, Sutiak V. Micronucleus frequency in cytokinesis-blocked bovine lymphocytes from regions with different pollution levels in Slovakia. Bull Environ Contam Toxicol 2001; 66: 449-455 https://doi.org/10.1007/s00128-001-0027-9
  29. Tamura H, Sakurai M, Sugahara T. Chromosome aberrations of the peripheral lymphocytes in rabbits exposed to single and fractionated whole-body X-irradiations. J Radiat Res 1978; 19: 108-114 https://doi.org/10.1269/jrr.19.108
  30. Tempel K. Chernobyl and its consequences-some veterinary medical points of view. Tierarztl Prax Ausg G Grosstiere Nutztiere 1997; 25: 401-405
  31. Thierens H, Vral A, Barbe M, Aousalah B, De Ridder L. A cytogenetic study of nuclear power plant workers using the micronucleus-centromere assay. Mutat Res 1999; 445: 105-111 https://doi.org/10.1016/S1383-5718(99)00134-5
  32. Thierens H, Vral A, Morthier R, Aousalah B, De Ridder L. Cytogenetic monitoring of hospital workers occupationally exposed to ionizing radiation using the micronucleus centromere assay. Mutagenesis 2000; 15:15: 245-249 https://doi.org/10.1093/mutage/15.3.245
  33. Thierens H, Vral A, Barbe M, Meijlaers M, Baeyens A, Ridder LD. Chromosomal radiosensitivity study of temporary nuclear workers and the support of the adaptive response induced by occupational exposure. Int J Radiat Biol 2002; 78: 1117-1126 https://doi.org/10.1080/0955300021000034710
  34. Thomson EJ, Perry PE. The identification of micronucleated chromosomes: a possible assay for aneuploid. Mutagenesis 1988; 3: 415-418 https://doi.org/10.1093/mutage/3.5.415
  35. Zuniga G, Torres-Bugarin O, Ramirez-Munoz MP, Ramos A, Fanti-Rodriguez E, Portilla E, Garcia-Martinez D, Cantu .JM, Gallegos-Arreola MP, Sanchez-Corona J. Spontaneous micronuclei in peripheral blood erythrocytes from 35 mammalian species. Mutat Res 1996; 369: 123-127 https://doi.org/10.1016/S0165-1218(96)90056-7
  36. Zuniga-Gonzalez G, Torres-Bugarin O, Zamora-Perez A, Gomez-Meda BC, Ramos-Ibarra ML, Martinez-Gonzalez S, Gonzalez-Rodriguez A, Luna-Aguirre J, Ramos-Mora A, Ontiveros-Lira D, Gallegos-Arreola MP. Differences in the number of micronucleated erythrocytes among young and adult animals including humans. Spontaneous micronuclei in 43 species. Mutat Res 2001; 494: 161-167 https://doi.org/10.1016/S1383-5718(01)00180-2