Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Effects of Dopamine.HCI on Structural Parameters of Bovine Brain Membranes

  • Bae, Moon-Kyoung (College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University) ;
  • Huh, Min-Hoi (College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University) ;
  • Lee, Seung-Woo (College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University) ;
  • Kang, Hyun-Gu (College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University) ;
  • Pyun, Jae-Ho (College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University) ;
  • Kwak, Myeong-Hee (College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University) ;
  • Jang, Hye-Ock (College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University) ;
  • Yun, Il (College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University)
  • Published : 2004.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

Fluorescence probes located in different membrane regions were used to evaluate the effect of dopamine$.$HCI on the structural parameters (transbilayer lateral mobility, annular lipid fluidity, protein distribution, and thickness of the lipid bilayer) of synaptosomal plasma membrane vesicles (SPMV), which were obtained from the bovine cerebral cortex. An experimental procedure was used based on selective quenching of 1,3-di(1-pyrenyl)propane (Py-3-Py) by trinitrophenyl groups, and radiationless energy transfer from the tryptophan of membrane pro-teins to Py-3-Py and energy transfer from Py-3-Py monomers to 1-anilinonaphthalene-8-sulfonic acid (ANS) was also utilized. Dopamine$.$HCI increased both the bulk lateral mobility and annular lipid fluidity, and it had a greater fluidizing effect on the inner monolayer than on the outer monolayer. Furthermore, the drug had a clustering effect on membrane proteins.

Keywords

References

  1. Chabanel, A., Abbott, R. E., Chien, S., and Schachter, D., Effects of benzyl alcohol on erythrocyte shape, membrane hemileaflet fluidity and membrane viscoelasticity. Biochim Biophys. Acta, 816, 142-152(1985) https://doi.org/10.1016/0005-2736(85)90402-X
  2. Cogan, U. and Schachter, D., Asymmetry of lipid dynamics in human erythrocyte membranes studied with impermeant fluorophores. Biochemistry, 20, 6396-6403 (1981) https://doi.org/10.1021/bi00525a018
  3. Cress, I., Burt, D. R., and Synder, S. H., Dopamine receptor binding predicts clinical and pharmacological potencies of antischizophrenic drugs. Science, 192, 481-483 (1976) https://doi.org/10.1126/science.3854
  4. Dobretsov, G. E., Spirin, M. M., Chekrygin, O. V., Karamansky, I. M., Dmitriev, V. M., and Vladimirov, Y. A., A fluorescence study of ap이poprotein localization in relation to lipids in serum low density lipoproteins. Biochim. Biophys. Acta, 710, 172-180(1982) https://doi.org/10.1016/0005-2760(82)90147-3
  5. Eisinger, J. and Flores, J., The relative locations of intramembrane fluorescent probes and of the cytosol hemoglobin in erythrocytes, studied by transverse resonance energy transfer. Biophys. J., 37, 6-7 (1982) https://doi.org/10.1016/S0006-3495(82)84571-2
  6. Faedda, G., Kula, N. S., and Baldessarini, R. J., Pharmacology of binding of 3H-SCH-23390 to D-1 dopaminergic receptor sites in rat striatal tissue. Biochem. Pharmacol., 38, 473-480 (1989) https://doi.org/10.1016/0006-2952(89)90387-0
  7. Kang, J. S., Choi, Ch. M., and Yun, I., Effects of ethanol on lateral and rotational mobility of plasma membrane vesicles isolated from cultured mouse myeloma cellline Sp2/0-Ag14. Biochim. Biophys. Acta, 1281, 157-163(1996) https://doi.org/10.1016/0005-2736(95)00301-0
  8. Kebabian, J. W. and Caine, D. B., Multiple receptors for dopamine. Nature, 277, 93-96 (1979) https://doi.org/10.1038/277093a0
  9. Kier, A. B., Sweet, W. D., Cowlen, M. S., and Schroeder, F., Regulation of transbilayer distribution of a fluorescent sterol in tumor cell plasma membranes. Biochim. Biophys. Acta, 861 ,287-301 (1986)
  10. Kim, H. G., Choi, C. H., Kim, I. S., Chung, I. K., Cho, G. J., Jang, H. O., and Yun, I., Different effects of dopamine on differential rotational mobility between inner and outer monolayer of synaptosomal plasma membrane vesicles isolated from bovine brain. Kor. J. Physiol. Pharmacol., 4, 409-415 (2000)
  11. Lang, F., Busch, G. L., Ritter, M., Volki, H., Waldegger, S., Gulbins, E., and Haussinger, D., Functional significance of cell volume regulatory mechanisms. Physiol. Rev, 78, 247-306 (1998) https://doi.org/10.1152/physrev.1998.78.1.247
  12. Lang, F., Ritter, M., Vblki, H., and Haussinger, D., The biological significance of cell volume. Renal. Physiol. Biochem., 16, 4865 (1993)
  13. Lee, S. M., The asymmetric effect of local anesthetics on transbilayer lateral and rotational mobility of model membranes of total phospholipid fraction extracted from brain membranes. Ph. D. Thesis. Pusan National University (1999)
  14. Lowry, O. H., Rosebrough, N. R., Farr, A. L., and Randall, R. G., Protein measurementwith the Folin reagent. J. Biol. Chem., 193, 265-275 (1951)
  15. Min, B. G., The asymmetric effect of local anesthetics on transbilayer lateral and rotational mobility of model membranes of totallipid fraction extractedfrom brain membranes Ph. D. Thesis. Pusan National University (1997)
  16. Peroutka, S. J. and Snyder, S. H., Long-term antidepressant treatment decreases spiroperidol-Iabeled serotonin receptor binding. Science, 210, 88-90 (1980) https://doi.org/10.1126/science.6251550
  17. Schachter, D., Fluidity and function of hepatocyte plasma membranes. Hepatology. 4, 140-151 (1984) https://doi.org/10.1002/hep.1840040124
  18. Schachter, D., Abott, R. E., Cogan, U., and Flamm, M., Lipid fluidity of the individual hemileaflets of human erythrocyte membranes. Ann. N. Y. Acd. Sci., 414, 19-28 (1983) https://doi.org/10.1111/j.1749-6632.1983.tb31671.x
  19. Schroeder, F., Jefferson, J. R., Kier, A. B., Knittel, J., Scallen, T. J., Wood, W. G., and Hapala, I., Membrane cholesterol dynamics: cholesterol domains and kinetic pools. Proc. Soc.Exp. Bio. Med., 196, 235-252 (1991a) https://doi.org/10.3181/00379727-196-43185
  20. Schroeder, F., Nemecz, G., Wood, W. G., Morrt, G., AyrautJarrier, M., and Devaux, P. F., Transmembrane distribution of sterol in the human erythrocyte. Biochim. Biophys. Acta,1066,183-192 (1991b) https://doi.org/10.1016/0005-2736(91)90185-B
  21. Seigneuret, M., Zachowski, A., Hermann, A., and Devaux, P. F., Asymmetric lipid fluidity in human erythrocyte membrane. Biochemistry, 23, 4271 -4275 (1984) https://doi.org/10.1021/bi00314a002
  22. Sheetz , M. P. and Singer, S. J., Biological membranes as bilayer couples. A molecular mechanism of drug-erythrocyte interactions. Proc. Natl. Acad. Sci. U.S.A., 71, 4457-4461 (1974) https://doi.org/10.1073/pnas.71.11.4457
  23. Shinitzky, M., Membrane fluidity in malignancy. Adversative and recuperative. Biochim. Biophys. Acta, 738,251-261 (1984)
  24. Spector, A. A. and Yorek, M. A., Membrane lipid composition and cellular function. J. Lipid Res. 26, 1015-1035 (1985)
  25. Toplak, H., Zuehlke, R., Loidl, S., Hermetler, A., Honegger, U. E., and Wiesmann, U. N., Single and multiple desipramine exposure of cultured cells. Biochem. Pharmacol., 39, 1437-1443 (1990) https://doi.org/10.1016/0006-2952(90)90425-K
  26. Wood, W. G., Schroeder, F., Hogy, L., Rao, A. M., and Nemecz, G., Asymmetric distribution of a fluorescent sterol in synaptic plasma membranes: effects of chronic ethanol consumption. Biochim. Biophys. Acta , 1025, 243-246 (1990) https://doi.org/10.1016/0005-2736(90)90103-U
  27. Yun, I. and Kang , J. S., The general lipid composition and aminophospholipid asymmetry of synaptosomal plasma membrane vesicles isolated from bovine cerebral cortex. Mol. Cells, 1, 15-20 (1990)
  28. Yun，I., Kim, Y. S., Yu, S. H., Chung , I. K., Kim, I. S., Baik, S. W., Cho, G. J., Chung , Y. Z., Kim, S. H., and Kang, J. S., Comparison of several procedures for the preparation of synaptosomal plasma membrane vesicles. Arch. Pharm. Res., 13, 325-329 (1990) https://doi.org/10.1007/BF02858167
  29. Yun, I., Lee, S.-H., and Kang, J. S., The effect of ethanol on lateral and rotational mobility of plasma membrane vesicles isolated from cultured Mar 18.5 hybridoma cells. J. Membr. Biol., 138, 221-227 (1994)
  30. Yun, I., Yang, M. S., Kim, I. S., and Kang, J. S., Bulk vs. transbilayer effects of ethanol on the fluidity of the plasma membrane vesicles of cultured Chinese hamster ovary cells. Asia Pacific J. Pharmacol., 8, 9-16 (1993)
  31. Zachariasse, K. A., Vaz, W. L. C., Sotomayer, C., and Kuhnle, W., Investigation of human erythrocyte ghost membranes with intramolecular excimer probes. Biochim. Biophys. Acta, 688, 323-332 (1982) https://doi.org/10.1016/0005-2736(82)90343-1
  32. Stubbs , C. D. and Williams , B. W., Fluorescence in membranes in Fluorescence Spectroscopy in Biochemistry, In Lakowicz, J. R. (Ed). Plenum Press, NewYork, pp. 231-263, (1991)